Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	126	206.4	6980590	99.2%	6924745	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,769,648	(C)_6→7	intergenic (+162/‑55)	yfjQ → / → yfjR	CP4‑57 prophage; protein YfjQ/CP4‑57 prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,769,642	1	.	C	96.4%	573.7 / 10.9	170	intergenic (+156/‑61)	yfjQ/yfjR	CP4‑57 prophage; protein YfjQ/CP4‑57 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base . (3/3); new base C (89/73); total (93/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 187 total aligned reads displayed.

TGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGA  >  NC_000913/2769506‑2769779

  ttAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTATCCCCCGTTACATCTGGCTTTTACAGAAATAAAAAATAGTTTCTGCGTTGTCCATTCCCTGTCCG‑‑ccccc                                                                                                                                      <  1:3141028/140‑1 (MQ=255)

  ttAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑‑ccccc                                                                                                                                      <  1:2124545/140‑1 (MQ=255)

       atCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCGTACCCTGTCCG‑‑ccccccctct                                                                                                                                 <  2:3342443/140‑5 (MQ=255)

        tCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑‑ccccccctctt                                                                                                                                <  2:534485/140‑6 (MQ=255)

         cGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCttt                                                                                                                                >  2:2145774/1‑140 (MQ=255)

          ggACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTa                                                                                                                               >  2:934068/1‑140 (MQ=255)

           gACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑‑ccccc                                                                                                                                      >  1:921414/1‑131 (MQ=255)

            acacCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTaaa                                                                                                                             >  2:3427027/1‑140 (MQ=255)

            acacCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTaaa                                                                                                                             >  1:2910276/1‑140 (MQ=255)

            acacCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTa                                                                                                                               >  1:3168657/1‑138 (MQ=255)

             caccacCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAg                                                                                                                            <  2:618127/140‑1 (MQ=255)

                caccTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑‑cccccc                                                                                                                                     <  1:2233655/127‑1 (MQ=255)

                 accTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAAt                                                                                                                        <  1:2534423/140‑1 (MQ=255)

                 accTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAAt                                                                                                                        >  1:3030749/1‑140 (MQ=255)

                  ccTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATc                                                                                                                       >  2:2094563/1‑140 (MQ=255)

                    tGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATcac                                                                                                                     <  2:1349261/140‑1 (MQ=255)

                    tGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATcac                                                                                                                     <  2:879572/140‑1 (MQ=255)

                      tCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAAt                                                                                                                        >  1:1519441/1‑135 (MQ=255)

                      tCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAAt                                                                                                                        <  2:1519441/135‑1 (MQ=255)

                      tCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCAcat                                                                                                                   <  2:2910276/140‑1 (MQ=255)

                      tCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCAcat                                                                                                                   >  2:3187766/1‑140 (MQ=255)

                      tCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCAcat                                                                                                                   >  2:118458/1‑140 (MQ=255)

                          cATCGCTATGTGCTCGTGTGCCTCAATCCTCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCAtt                                                                                                               >  2:1707025/1‑140 (MQ=255)

                          cATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCAtt                                                                                                               >  1:7025/1‑140 (MQ=255)

                             cGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATGGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTtc                                                                                                            >  2:638927/1‑140 (MQ=255)

                             cGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTtc                                                                                                            >  1:2790422/1‑140 (MQ=255)

                             cGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTAAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTtc                                                                                                            >  1:2336904/1‑140 (MQ=255)

                              gCTATGTGCT‑‑TGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTtcagt                                                                                                         <  2:3094338/140‑1 (MQ=21)

                                tATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTtcagt                                                                                                         <  2:1876072/140‑1 (MQ=255)

                                tATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTtcagt                                                                                                         <  1:241705/140‑1 (MQ=255)

                                      cTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTa                                                                                                   >  1:3288456/1‑140 (MQ=255)

                                       tCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCCTCTTTAAAGTAATCACATCATTTtca                                                                                                           >  1:552240/1‑132 (MQ=255)

                                       tCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                                                                  >  2:2574925/1‑140 (MQ=255)

                                       tCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                                                                  <  1:1814845/140‑1 (MQ=255)

                                       tCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                                                                  <  2:1662616/140‑1 (MQ=255)

                                       tCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                                                                  <  1:3326058/140‑1 (MQ=255)

                                       tCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                                                                  >  2:1998242/1‑140 (MQ=255)

                                         gtgtGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGT‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACt                                                                                                >  2:1897967/1‑140 (MQ=255)

                                         gtgtGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACt                                                                                                >  1:1627917/1‑140 (MQ=255)

                                          tgtgCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTtca                                                                                                           <  2:2992787/128‑1 (MQ=255)

                                          tgtgCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACtt                                                                                               >  1:2060562/1‑140 (MQ=255)

                                             gCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTcc                                                                                            <  2:2790422/140‑1 (MQ=255)

                                             gCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTcc                                                                                            <  2:3288456/140‑1 (MQ=255)

                                                tCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTgg                                                                                         <  1:991929/140‑1 (MQ=255)

                                                   aTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGgaga                                                                                      <  2:2016616/140‑1 (MQ=255)

                                                    tCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGaa                                                                                     <  1:118458/140‑1 (MQ=255)

                                                    tCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGaa                                                                                     <  2:2705978/140‑1 (MQ=255)

                                                    tCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGaa                                                                                     <  1:1151985/140‑1 (MQ=255)

                                                         cGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctct                                                                                >  1:1760677/1‑140 (MQ=255)

                                                          ggTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGT‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctctc                                                                               <  1:1897967/140‑1 (MQ=255)

                                                          ggTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTcc                                                                                            <  1:3346433/127‑1 (MQ=255)

                                                            ttATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCAt                                                                             <  1:1707025/140‑1 (MQ=255)

                                                            ttATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCAt                                                                             >  1:1369153/1‑140 (MQ=255)

                                                               taGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGac                                                                          <  2:2348918/140‑1 (MQ=255)

                                                                      tAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCa                                                                   <  2:7025/140‑1 (MQ=255)

                                                                             cGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAcgcc                                                            >  2:863975/1‑140 (MQ=255)

                                                                              gTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAcgccg                                                           >  2:2762774/1‑140 (MQ=255)

                                                                                    tCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGa                                                     <  2:1441109/140‑1 (MQ=255)

                                                                                       ggCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCg                                                  >  2:95515/1‑140 (MQ=255)

                                                                                        gCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCgg                                                 >  2:339664/1‑140 (MQ=255)

                                                                                        gCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTAATGACACAGGCAGAACGCCGCCATGACCgg                                                 >  2:328406/1‑140 (MQ=255)

                                                                                          ttttGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAAAACGCCGCCATGACCggct                                               <  2:2336904/140‑1 (MQ=255)

                                                                                            ttGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCggctgg                                             <  1:2574925/140‑1 (MQ=255)

                                                                                             tGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCggctggc                                            >  2:2806513/1‑140 (MQ=255)

                                                                                             tGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCggctggc                                            >  1:2839424/1‑140 (MQ=255)

                                                                                               cAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGgctggctg                                          >  1:3455513/1‑140 (MQ=255)

                                                                                                aGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgt                                         <  2:2500693/140‑1 (MQ=255)

                                                                                                aGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgt                                         <  1:638927/140‑1 (MQ=255)

                                                                                                aGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgt                                         <  2:1369153/140‑1 (MQ=255)

                                                                                                  aaaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCggct                                               >  1:446994/1‑132 (MQ=255)

                                                                                                   aaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcagg                                     <  2:394415/140‑1 (MQ=255)

                                                                                                   aaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcagg                                     <  2:2928492/140‑1 (MQ=255)

                                                                                                    aTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcagg                                     <  1:3427027/140‑1 (MQ=255)

                                                                                                           aTAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCAc                              <  1:1998242/140‑1 (MQ=255)

                                                                                                            tAGTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACt                             >  2:3480787/1‑140 (MQ=255)

                                                                                                              gTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcaggctgt                                 <  2:3299050/134‑1 (MQ=255)

                                                                                                              gTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcaggctgt                                 <  2:3173217/134‑1 (MQ=255)

                                                                                                              gTTTCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtca                                       >  1:701014/1‑128 (MQ=255)

                                                                                                               tttCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcaggct                                   <  2:795824/131‑1 (MQ=255)

                                                                                                                ttCTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATa                         >  1:3336150/1‑140 (MQ=255)

                                                                                                                  cTGCGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAAt                       <  1:863975/140‑1 (MQ=255)

                                                                                                                    ccGTTTTCCACGCCCTATCCG‑‑CCCCCCTCTTTAAAGAAATCACATCATTTTCAGTCAATTACCTTTCCTGGGGAATCTCTCATGCCACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAg                    <  2:3142151/139‑1 (MQ=14)

                                                                                                                     cGTTGTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAg                    <  1:3480787/140‑1 (MQ=255)

                                                                                                                         gTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGt                <  1:2094563/140‑1 (MQ=255)

                                                                                                                         gTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGt                >  1:1852712/1‑140 (MQ=255)

                                                                                                                         gTCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGt                >  1:142977/1‑140 (MQ=255)

                                                                                                                          tCCATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTc               >  1:2767623/1‑140 (MQ=255)

                                                                                                                           ccATACCCTGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCt              <  1:2012473/140‑1 (MQ=255)

                                                                                                                                   tGTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTAATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCgg      <  1:328406/140‑1 (MQ=255)

                                                                                                                                    gTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCggg     >  1:150233/1‑140 (MQ=255)

                                                                                                                                    gTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCggg     >  1:2471503/1‑140 (MQ=255)

                                                                                                                                    gTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCggg     >  1:2069077/1‑140 (MQ=255)

                                                                                                                                    gTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCggg     >  2:2968908/1‑140 (MQ=255)

                                                                                                                                    gTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCggg     >  1:2708644/1‑140 (MQ=255)

                                                                                                                                    gTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCggg     >  1:2984041/1‑140 (MQ=255)

                                                                                                                                    gTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCggg     >  1:3184828/1‑140 (MQ=255)

                                                                                                                                    gTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCggg     >  1:321555/1‑140 (MQ=255)

                                                                                                                                    gTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCggg     >  1:94348/1‑140 (MQ=255)

                                                                                                                                    gTCCGC‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCggg     >  1:794561/1‑140 (MQ=255)

                                                                                                                                      ccG‑‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGa  <  2:1814776/140‑1 (MQ=255)

TGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGA  >  NC_000913/2769506‑2769779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A