Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	126	206.4	6980590	99.2%	6924745	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,835,043	T→C	*378Q (TAG→CAG)	norW →	NADH:flavorubredoxin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,835,043	0	T	C	99.1%	405.2 / ‑3.7	113	coding (1132/1134 nt)	norW	NADH:flavorubredoxin reductase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (54/58); minor base . (1/0); total (55/58)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 114 total aligned reads displayed.

ATTTACGCTGGCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTATCCG  >  NC_000913/2834906‑2835179

aTTTACGCTGGCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAg                                                                                                                                        <  1:2336130/140‑1 (MQ=255)

   tACGCTGGCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTg                                                                                                                                     >  1:70022/1‑140 (MQ=255)

   tACGCTGGCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTg                                                                                                                                     >  1:1002087/1‑140 (MQ=255)

     cGCTGGCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTggg                                                                                                                                   >  1:2557862/1‑140 (MQ=255)

     cGCTGGCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTggg                                                                                                                                   <  2:2860651/140‑1 (MQ=255)

         ggCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTAc                                                                                                                               >  1:719305/1‑140 (MQ=255)

         ggCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTAc                                                                                                                               >  1:1407533/1‑140 (MQ=255)

          gCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACt                                                                                                                              <  1:1163933/140‑1 (MQ=255)

           cAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACtg                                                                                                                             <  1:224664/140‑1 (MQ=255)

           cAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACtg                                                                                                                             <  2:1126854/140‑1 (MQ=255)

            aaaTTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACtgt                                                                                                                            <  1:3194435/140‑1 (MQ=255)

                        aaCGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTAc                                                                                                                               <  1:2398707/125‑1 (MQ=255)

                        aaCGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTAc                                                                                                                               >  2:2398707/1‑125 (MQ=255)

                        aaCGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTc                                                                                                                >  1:415102/1‑140 (MQ=255)

                        aaCGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTc                                                                                                                <  2:3087460/140‑1 (MQ=255)

                        aaCGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTc                                                                                                                >  2:666393/1‑140 (MQ=255)

                         aCGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTAc                                                                                                                               >  2:2032989/1‑124 (MQ=255)

                         aCGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTAc                                                                                                                               <  1:2032989/124‑1 (MQ=255)

                         aCGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCg                                                                                                               >  1:2433507/1‑140 (MQ=255)

                         aCGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCg                                                                                                               <  1:1370844/140‑1 (MQ=255)

                         aCGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTATTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCg                                                                                                               >  1:2775400/1‑140 (MQ=255)

                          cGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCgg                                                                                                              >  2:2156348/1‑140 (MQ=255)

                          cGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCgg                                                                                                              >  1:103756/1‑140 (MQ=255)

                           gCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGGCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTAGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGa                                                                                                             <  2:413774/140‑1 (MQ=255)

                           gCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGa                                                                                                             <  2:2670662/140‑1 (MQ=255)

                           gCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGa                                                                                                             <  2:70022/140‑1 (MQ=255)

                           gCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGa                                                                                                             <  2:1175696/140‑1 (MQ=255)

                            ccAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGAt                                                                                                            >  1:244173/1‑140 (MQ=255)

                             cAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATg                                                                                                           <  1:870037/140‑1 (MQ=255)

                               gggAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCg                                                                                                         >  1:3481915/1‑140 (MQ=255)

                                  aaTGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACg                                                                                                      <  1:2156348/140‑1 (MQ=255)

                                     ggtggCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTg                                                                                                   >  1:1034403/1‑140 (MQ=255)

                                     ggtggCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTg                                                                                                   >  1:1589487/1‑140 (MQ=255)

                                     ggtggCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTg                                                                                                   >  1:961556/1‑140 (MQ=255)

                                      gtggCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTgg                                                                                                  <  1:2614079/140‑1 (MQ=255)

                                      gtggCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTgg                                                                                                  <  2:2185670/140‑1 (MQ=255)

                                      gtggCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTATTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTgg                                                                                                  <  2:2775400/140‑1 (MQ=255)

                                       tggCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGgc                                                                                                 >  2:1579487/1‑140 (MQ=255)

                                       tggCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGgc                                                                                                 <  2:1428241/140‑1 (MQ=255)

                                       tggCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGgc                                                                                                 >  1:919448/1‑140 (MQ=255)

                                          cgcgcgGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGcgcg                                                                                              <  1:237160/140‑1 (MQ=255)

                                          cgcgcgGCGTTGACGATGCTGACCAGCTTCACGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGgc                                                                                                 >  1:2070389/1‑137 (MQ=255)

                                           gcgcgGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGt                                                                                             >  1:3265646/1‑140 (MQ=255)

                                           gcgcgGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGATCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGt                                                                                             <  2:3018225/140‑1 (MQ=255)

                                            cgcgGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGt                                                                                             <  2:3262539/139‑1 (MQ=255)

                                            cgcgGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGt                                                                                             >  1:3262539/1‑139 (MQ=255)

                                            cgcgGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTc                                                                                            >  2:278151/1‑140 (MQ=255)

                                              cggcgTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGACGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCtt                                                                                          >  1:1041161/1‑140 (MQ=255)

                                                   ttGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCg                                                                                     <  2:3481915/140‑1 (MQ=255)

                                                     gACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGAc                                                                                   <  2:3265646/140‑1 (MQ=255)

                                                         aTGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTAc                                                                               <  2:919448/140‑1 (MQ=255)

                                                           gCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTAc                                                                               >  1:976306/1‑138 (MQ=255)

                                                           gCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACgg                                                                             >  1:1907823/1‑140 (MQ=255)

                                                           gCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACgg                                                                             <  1:2433678/140‑1 (MQ=255)

                                                            cTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACggg                                                                            <  2:2882374/140‑1 (MQ=255)

                                                              gACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAACCATTGCCGATGCAGGTTGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGGCCTACGGGGa                                                                          <  2:2637007/140‑1 (MQ=255)

                                                              gACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCg                                                                                     >  1:197032/1‑129 (MQ=255)

                                                              gACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCg                                                                                     <  2:197032/129‑1 (MQ=255)

                                                              gACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGa                                                                          <  1:278151/140‑1 (MQ=255)

                                                              gACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGa                                                                          <  1:1107246/140‑1 (MQ=255)

                                                              gACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGa                                                                          <  1:2889058/140‑1 (MQ=255)

                                                              gACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGa                                                                          <  2:1907823/140‑1 (MQ=255)

                                                              gACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGa                                                                          <  2:1002087/140‑1 (MQ=255)

                                                               aCCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGAc                                                                         >  1:2994808/1‑140 (MQ=255)

                                                                ccAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACggg                                                                            <  1:205260/136‑1 (MQ=255)

                                                                ccAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACggg                                                                            >  2:205260/1‑136 (MQ=255)

                                                                ccAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACg                                                                        <  2:2557862/140‑1 (MQ=255)

                                                                    cTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGAc                                                                                   >  2:803572/1‑125 (MQ=255)

                                                                    cTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGAc                                                                                   <  1:803572/125‑1 (MQ=255)

                                                                      tCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCAtgtg                                                                  >  1:1792260/1‑140 (MQ=255)

                                                                                      aGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGAt                                                        <  1:1122558/134‑1 (MQ=255)

                                                                                      aGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGAt                                                        >  2:1122558/1‑134 (MQ=255)

                                                                                      aGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCg                                                  >  2:1172622/1‑140 (MQ=255)

                                                                                        tGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGtt                                                >  2:1770379/1‑140 (MQ=255)

                                                                                        tGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGtt                                                >  1:360442/1‑140 (MQ=255)

                                                                                        tGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGtt                                                >  2:1668160/1‑140 (MQ=255)

                                                                                        tGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGtt                                                >  1:250343/1‑140 (MQ=255)

                                                                                             aTCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTAcgc                                           >  2:1631575/1‑140 (MQ=255)

                                                                                             aTCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTAcgc                                           >  1:40184/1‑140 (MQ=255)

                                                                                                 gATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCa                                       <  2:103756/140‑1 (MQ=255)

                                                                                                 gATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCa                                       >  1:3113499/1‑140 (MQ=255)

                                                                                                 gATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCa                                       <  2:2615531/140‑1 (MQ=255)

                                                                                                  aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCAt                                      >  1:2597967/1‑140 (MQ=255)

                                                                                                  aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCAt                                      >  1:1727835/1‑140 (MQ=255)

                                                                                                  aTGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCCGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCAt                                      >  1:2883689/1‑140 (MQ=255)

                                                                                                   tGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATc                                     <  1:1172622/140‑1 (MQ=255)

                                                                                                            cATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                   <  1:3234888/133‑1 (MQ=255)

                                                                                                            cATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                   >  2:3234888/1‑133 (MQ=255)

                                                                                                            cATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATgg                            >  2:2981916/1‑140 (MQ=255)

                                                                                                            cATTTGGATTGTTGAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATgg                            <  1:1668160/140‑1 (MQ=255)

                                                                                                                    ttgttgAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCaaa                    <  2:2165017/140‑1 (MQ=255)

                                                                                                                      gttgAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATg                  >  1:3035302/1‑140 (MQ=255)

                                                                                                                         gAAAACATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCaa               <  2:1041161/140‑1 (MQ=255)

                                                                                                                         gAAAACATTGCCGATGCAGGTGGCCTACTGTGCCTAAAATGTCGGATGCCACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCaa               <  2:1387098/140‑1 (MQ=255)

                                                                                                                             aCATTGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACg           >  1:592696/1‑140 (MQ=255)

                                                                                                                                 tGCCGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGtttt       >  2:2907798/1‑140 (MQ=255)

                                                                                                                                   ccGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTAt     <  2:408280/140‑1 (MQ=255)

                                                                                                                                   ccGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTAt     <  1:1631575/140‑1 (MQ=255)

                                                                                                                                   ccGATGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTAt     <  2:3113499/140‑1 (MQ=255)

                                                                                                                                      aTGCAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTATCCg  <  2:774994/140‑1 (MQ=255)

ATTTACGCTGGCAAATTAATACCGAACGCCAGGGAATGGTGGCGCGCGGCGTTGACGATGCTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCATTTGGATTGTTGAAAACATTGCCGATGTAGGTGGGCTACTGTGCCTAAAATGTCGGATGCGACGCTGGCGCGTCTTATCCGACCTACGGGGACGCATGTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGTCCAAATGCAACACGTTTTATCCG  >  NC_000913/2834906‑2835179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A