Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,837,853	T→C	G214G (GGT→GGC)	ynjC →	putative ABC transporter membrane subunit YnjC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,837,853	0	T	C	100.0%	106.4 / NA	31	G214G (GGT→GGC)	ynjC	putative ABC transporter membrane subunit YnjC
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/16); total (15/16)

GCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTAT  >  NC_000913/1837718‑1837982

gCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATc                                                                                                                               >  1:91210/1‑140 (MQ=255)

         cAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGc                                                                                                                      <  1:304074/140‑1 (MQ=255)

         cAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGc                                                                                                                      <  1:745103/140‑1 (MQ=255)

                      tGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAg                                                                                                         >  2:713924/1‑140 (MQ=255)

                            aaCTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTTGCa                                                                                                   >  2:381219/1‑140 (MQ=255)

                            aaCTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCa                                                                                                   <  2:244329/140‑1 (MQ=255)

                            aaCTGGCTGCTGTTGCCCTCCGTCGCACCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCa                                                                                                   <  1:401501/140‑1 (MQ=255)

                                 gctgctGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCa                                                                                                   <  2:715432/135‑1 (MQ=255)

                                 gctgctGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCa                                                                                                   >  1:715432/1‑135 (MQ=255)

                                 gctgctGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGt                                                                                              <  1:552231/140‑1 (MQ=255)

                                 gctgctGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGt                                                                                              >  2:788803/1‑140 (MQ=255)

                                   tgctgTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCa                                                                                                   <  1:247369/133‑1 (MQ=255)

                                   tgctgTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCa                                                                                                   >  2:247369/1‑133 (MQ=255)

                                           cccTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAggg                                                                                    >  2:59771/1‑140 (MQ=255)

                                               ccGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGAc                                                                                >  1:674114/1‑140 (MQ=255)

                                                                gCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAAcaaacaaa                                                               >  1:448595/1‑140 (MQ=255)

                                                                     ggCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGgcg                                                          >  2:84916/1‑140 (MQ=255)

                                                                                cGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGc                                               <  1:65263/140‑1 (MQ=255)

                                                                                      ttgCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCctgctg                                         >  2:461746/1‑140 (MQ=255)

                                                                                        gCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGtt                                       <  1:788803/140‑1 (MQ=255)

                                                                                          cTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGa                                     <  1:84916/140‑1 (MQ=255)

                                                                                          cTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGa                                     <  2:448595/140‑1 (MQ=255)

                                                                                          cTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGa                                     <  1:59771/140‑1 (MQ=255)

                                                                                            ggTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATg                                   <  2:213352/140‑1 (MQ=255)

                                                                                                     cggtcgTGGATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATGCTGTTACTc                          <  2:91210/140‑1 (MQ=255)

                                                                                                            ggATGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATGCTGTTACTCGCCGCCt                   >  2:760329/1‑140 (MQ=255)

                                                                                                              aTGTGGCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATGCTGTTACTCGCCGCCTAc                 >  1:417283/1‑140 (MQ=255)

                                                                                                                 tgGCAATTATGCTCGGGCCAGGCAATCCCCCGACGCTGGCGGGAATTAGCTGGCAGTGGTTAACCCAGGGCGACAGTGACCAACAAACAAAAGGCGCGCTTGCCAACCTGCTGTTTATGCGGTTACCCACCGCCTACGtt              >  2:824505/1‑140 (MQ=255)

                                                                                                                  ggCAATTATTCTCGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATGCTGTTACTCGCCGCCTACGttt             >  2:336516/1‑140 (MQ=255)

                                                                                                                        tattCTCGGGCCAGGCAATCCCCCGACG‑TTGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATGCTGTTACTCGCCGCCTACGTTTTGCTGAg      <  2:456443/140‑1 (MQ=255)

                                                                                                                           tctcGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCt    <  1:18703/140‑1 (MQ=255)

                                                                                                                             tcGGGCCAGGCAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTAt  <  1:824505/140‑1 (MQ=255)

GCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCCAGGGCGACATTGACCAACAAACAAAAGGCGCGCTTGCCAGCCTGCTGTTGATGCTGTTACTCGCCGCCTACGTTTTGCTGAGCTAT  >  NC_000913/1837718‑1837982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A