Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 252 48.5 1661298 98.8% 1641362 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,969,994 T→C T331A (ACA→GCA)  tap ← methyl‑accepting chemotaxis protein Tap

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,969,9940TC100.0% 100.8 / NA 30T331A (ACA→GCA) tapmethyl‑accepting chemotaxis protein Tap
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/13);  total (17/13)

GGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGTACGACTTGAGAGA  >  NC_000913/1969860‑1970127
                                                                                                                                      |                                                                                                                                     
ggCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCgg                                                                                                                                  >  2:485861/1‑140 (MQ=255)
ggCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCgg                                                                                                                                  <  1:87074/140‑1 (MQ=255)
ggCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCgg                                                                                                                                  <  1:485377/140‑1 (MQ=255)
ggCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCgg                                                                                                                                  >  1:572719/1‑140 (MQ=255)
          ttGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTTAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGc                                                                                                                        >  1:630868/1‑140 (MQ=255)
                   aaaGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGa                                                                                                               <  1:819676/140‑1 (MQ=255)
                                         tGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCg                                                                                         >  2:378450/1‑140 (MQ=255)
                                           aTAATGTCGCCATTTTTTTGCGAGCTGGGGGCGATCTCCTGCGTGGTGTGAGTCAGGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGtt                                                                                       <  2:430085/140‑1 (MQ=255)
                                           aTAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGtt                                                                                       <  2:572719/140‑1 (MQ=255)
                                                   gCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTa                                                                               <  1:213315/140‑1 (MQ=255)
                                                   gCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTa                                                                               >  2:52839/1‑140 (MQ=255)
                                                         tttttGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTgg                                                                         <  1:290933/140‑1 (MQ=255)
                                                             tGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGt                                                                     >  2:652602/1‑140 (MQ=255)
                                                                   cTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTg                                                               <  1:652602/140‑1 (MQ=255)
                                                                         gCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCAt                                                         >  2:363444/1‑140 (MQ=255)
                                                                            aTCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACt                                                      >  1:384753/1‑140 (MQ=255)
                                                                                     aTGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGt                                             <  1:378450/140‑1 (MQ=255)
                                                                                         tgtgAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTtgt                                         >  2:79609/1‑140 (MQ=255)
                                                                                         tgtgAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTtgt                                         >  1:420226/1‑140 (MQ=255)
                                                                                         tgtgAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTtgt                                         <  1:178420/140‑1 (MQ=255)
                                                                                                     tACTGACCTGAACACCGCCCACCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGGTCATTCCTCCATAATGGGGGCCGCTTTGGCCCGCGATgcc                             >  2:367362/1‑140 (MQ=255)
                                                                                                        tGACCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATgccgcc                          >  1:588437/1‑140 (MQ=255)
                                                                                                          aCCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCctg                        >  2:291724/1‑140 (MQ=255)
                                                                                                          aCCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCctg                        <  1:456899/140‑1 (MQ=255)
                                                                                                          aCCTGAACACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCctg                        >  2:73431/1‑140 (MQ=255)
                                                                                                               aaCACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGtt                   >  2:437122/1‑140 (MQ=255)
                                                                                                               aaCACCGCCCGCCTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGtt                   >  2:523597/1‑140 (MQ=255)
                                                                                                                    cgcccgccTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGTa              >  1:276656/1‑140 (MQ=255)
                                                                                                                    cgcccgccTGCGCCGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGTa              <  1:252740/140‑1 (MQ=255)
                                                                                                                                ccGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGTACGACTTgagaga  <  1:52839/140‑1 (MQ=255)
                                                                                                                                ccGTTGCCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGTACGACTTgagaga  >  1:26164/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                     
GGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTGCATGGTGTGAGTCATGGTACTGACCTGAACACCGCCCGCCTGCGCCGTTGTCGCGGCATTTTTTGCCAGTTCCGACGCCTGTCGTGCGTTATCGGCGTTTTGCCCTACCGTGGCGGTTAATTGCTCCATACTGGCGGCCGTTTGTGCCAGCGATGCCGCCTGCTGTTCGGTACGACTTGAGAGA  >  NC_000913/1969860‑1970127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A