Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 252 48.5 1661298 98.8% 1641362 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,112,219 T→C V281V (GTA→GTG)  rfbB ← dTDP‑glucose 4,6‑dehydratase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,112,2190TC100.0% 212.0 / NA 60V281V (GTA→GTG) rfbBdTDP‑glucose 4,6‑dehydratase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/34);  total (26/34)

CCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGTACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTC  >  NC_000913/2112085‑2112353
                                                                                                                                      |                                                                                                                                      
ccGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAAt                                                                                                                                   <  2:47796/140‑1 (MQ=255)
 cGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAAtc                                                                                                                                  <  1:567020/140‑1 (MQ=255)
   ctctCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAAtctc                                                                                                                                >  2:677725/1‑140 (MQ=255)
        aaaCGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCa                                                                                                                           >  2:111622/1‑140 (MQ=255)
          aCGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGc                                                                                                                         <  1:122958/140‑1 (MQ=255)
            gTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCaa                                                                                                                       <  2:55004/140‑1 (MQ=255)
                ccTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATca                                                                                                                   >  1:112431/1‑140 (MQ=255)
                ccTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATca                                                                                                                   >  2:799887/1‑140 (MQ=255)
                 cTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATcac                                                                                                                  <  2:197696/140‑1 (MQ=255)
                   gtgGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACaa                                                                                                                <  2:77909/140‑1 (MQ=255)
                      gTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATa                                                                                                             <  1:159491/140‑1 (MQ=255)
                        ttCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGt                                                                                                           >  1:556161/1‑140 (MQ=255)
                        ttCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGt                                                                                                           <  1:585859/140‑1 (MQ=255)
                        ttCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGt                                                                                                           <  2:121784/140‑1 (MQ=255)
                         tCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTg                                                                                                          >  1:111157/1‑140 (MQ=255)
                           cATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAg                                                                                                        <  1:348985/140‑1 (MQ=255)
                           cATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAg                                                                                                        <  2:692371/140‑1 (MQ=255)
                                cAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTa                                                                                                   >  2:110432/1‑140 (MQ=255)
                                   tGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACAt                                                                                                >  2:722548/1‑140 (MQ=255)
                                     cgcgACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCg                                                                                              <  1:224905/140‑1 (MQ=255)
                                                    cAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCg                                                                               >  1:667062/1‑140 (MQ=255)
                                                            atagcatagcGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGccc                                                                       >  1:51334/1‑140 (MQ=255)
                                                            atagcatagcGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGccc                                                                       >  2:418006/1‑140 (MQ=255)
                                                            atagcatagcGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGccc                                                                       >  2:90907/1‑140 (MQ=255)
                                                              agcatagcGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCccac                                                                     <  1:665420/140‑1 (MQ=255)
                                                               gcatagcGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCccacc                                                                    >  2:425649/1‑140 (MQ=255)
                                                                catagcGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCccacca                                                                   <  2:51334/140‑1 (MQ=255)
                                                                   agcGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATg                                                                >  2:629826/1‑140 (MQ=255)
                                                                     cggcgATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGtt                                                              >  1:99559/1‑140 (MQ=255)
                                                                     cggcgATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGtt                                                              >  1:500269/1‑140 (MQ=255)
                                                                     cggcgATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGtt                                                              >  2:218453/1‑140 (MQ=255)
                                                                      ggcgATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTta                                                             <  1:677725/140‑1 (MQ=255)
                                                                       gcgATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTtat                                                            >  2:443203/1‑140 (MQ=255)
                                                                          aTCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCccacc                                                                    >  1:169104/1‑129 (MQ=255)
                                                                          aTCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCccacc                                                                    <  2:169104/129‑1 (MQ=255)
                                                                          aTCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAg                                                         >  1:800075/1‑140 (MQ=255)
                                                                                          tCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTAcc                                         <  1:722548/140‑1 (MQ=255)
                                                                                                aCATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGt                                   <  2:667062/140‑1 (MQ=255)
                                                                                                aCATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGt                                   >  1:462218/1‑140 (MQ=255)
                                                                                                  aTAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTa                                 <  1:359569/140‑1 (MQ=255)
                                                                                                  aTAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTa                                 <  2:500269/140‑1 (MQ=255)
                                                                                                  aTAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTa                                 <  1:630519/140‑1 (MQ=255)
                                                                                                  aTAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTa                                 <  1:425649/140‑1 (MQ=255)
                                                                                                     aGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTacga                              <  1:218453/140‑1 (MQ=255)
                                                                                                     aGTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTacga                              <  2:428331/140‑1 (MQ=255)
                                                                                                      gTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTacgac                             <  1:629826/140‑1 (MQ=255)
                                                                                                      gTGATTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTacgac                             <  2:556161/140‑1 (MQ=255)
                                                                                                         aTTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTa                                 <  2:222604/133‑1 (MQ=255)
                                                                                                         aTTTGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTa                                 >  1:222604/1‑133 (MQ=255)
                                                                                                           ttGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTacg                               >  1:626497/1‑133 (MQ=255)
                                                                                                           ttGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTacg                               <  2:626497/133‑1 (MQ=255)
                                                                                                            tGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTacgacg                            <  2:789818/135‑1 (MQ=255)
                                                                                                            tGCTCACGATAAGATTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTacgacg                            >  1:789818/1‑135 (MQ=255)
                                                                                                                         aTTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCa          <  1:111622/140‑1 (MQ=255)
                                                                                                                         aTTTCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCa          <  2:112431/140‑1 (MQ=255)
                                                                                                                           ttCTCTTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATg        <  2:809019/140‑1 (MQ=255)
                                                                                                                             ctctTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCa          <  2:433179/136‑1 (MQ=255)
                                                                                                                             ctctTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCa          >  1:433179/1‑136 (MQ=255)
                                                                                                                               ctTTCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGATGGTATATAACGCACGCGCATGATCt    >  1:480008/1‑140 (MQ=255)
                                                                                                                                 ttCGGCACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTc  <  2:99559/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CCGCTCTCAAACGTTTCCTGTGGTTTCCATCCCAATGCGCGACCAATCTTCTCAGCATCAATAGCATAGCGGCGATCGTGTCCCGGACGATCAGCAACATAAGTGATTTGCTCACGATAAGATTTCTCTTTCGGTACAATCTCATCCAGCAAATCACAAATAGTGAGCACTACATCGATGTTTTTCTTTTCGTTGTGCCCACCAATGTTATAAGTTTCACCCGCTTTACCTTCGGTTACGACGGTATATAACGCACGCGCATGATCTTC  >  NC_000913/2112085‑2112353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A