Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,117,585	Δ1 bp	coding (820/1281 nt)	wcaK ←	putative colanic acid biosynthesis pyruvyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,117,585	0	A	.	100.0%	178.3 / NA	43	coding (820/1281 nt)	wcaK	putative colanic acid biosynthesis pyruvyl transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (24/19); total (24/19)

TGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACGT  >  NC_000913/2117456‑2117719

tGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCga                                                                                                                             >  1:42823/1‑140 (MQ=255)

                 aCGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCACAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGaccacc                                                                                                            <  2:219891/140‑1 (MQ=255)

                  cGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCAccc                                                                                                           >  2:23929/1‑140 (MQ=255)

                       tAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCa                                                                                                      >  1:584130/1‑140 (MQ=255)

                          cGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAg                                                                                                   >  1:439693/1‑140 (MQ=255)

                          cGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAg                                                                                                   >  1:10251/1‑140 (MQ=255)

                          cGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAg                                                                                                   >  1:489485/1‑140 (MQ=255)

                                 gATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCttttt                                                                                            >  1:614833/1‑140 (MQ=255)

                                       tGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACg                                                                                      <  2:614833/140‑1 (MQ=255)

                                        gATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGc                                                                                     <  1:421253/140‑1 (MQ=255)

                                            tgCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGTTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTAATACGCttgt                                                                                 >  1:568467/1‑140 (MQ=255)

                                             gCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCttgtt                                                                                <  2:42823/140‑1 (MQ=255)

                                                ggCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAg                                                                             <  2:542097/140‑1 (MQ=255)

                                                      ggTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGc                                                                       >  1:634567/1‑140 (MQ=255)

                                                       gTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGcc                                                                      <  2:726695/140‑1 (MQ=255)

                                                           aGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCgaga                                                                  >  1:16361/1‑140 (MQ=255)

                                                              gcCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGt                                                               <  2:548341/140‑1 (MQ=255)

                                                                      gCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCaaa                                                       <  2:584130/140‑1 (MQ=255)

                                                                      gCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCaaa                                                       <  1:23929/140‑1 (MQ=255)

                                                                          tcgtcTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGt                                                   >  2:483152/1‑140 (MQ=255)

                                                                            gtcTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGc                                                 >  2:417050/1‑140 (MQ=255)

                                                                                ttGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGt                                             <  2:634567/140‑1 (MQ=255)

                                                                                 tGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCaaa                                                       <  1:74325/129‑1 (MQ=255)

                                                                                 tGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCaaa                                                       >  2:74325/1‑129 (MQ=255)

                                                                                       aGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCa                                      <  1:677381/140‑1 (MQ=255)

                                                                                           gTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGt                                  >  2:329160/1‑140 (MQ=255)

                                                                                            tCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTa                                 >  2:144260/1‑140 (MQ=255)

                                                                                             cAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTcgcg                                        <  1:826503/132‑1 (MQ=255)

                                                                                             cAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTcgcg                                        >  2:826503/1‑132 (MQ=255)

                                                                                             cAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTaa                                >  2:31517/1‑140 (MQ=255)

                                                                                                           gTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTc                  >  2:370396/1‑140 (MQ=255)

                                                                                                           gTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTAATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTc                  >  2:308798/1‑140 (MQ=255)

                                                                                                             ggAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTc                  <  1:84980/138‑1 (MQ=255)

                                                                                                             ggAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTc                  >  2:84980/1‑138 (MQ=255)

                                                                                                             ggAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCtg                >  1:776177/1‑140 (MQ=255)

                                                                                                             ggAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCtg                <  1:483152/140‑1 (MQ=255)

                                                                                                                   cgcgaTCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGc          >  1:812055/5‑140 (MQ=255)

                                                                                                                   cgcAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGc          >  1:488530/1‑140 (MQ=255)

                                                                                                                   cgcAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGc          >  2:807707/1‑140 (MQ=255)

                                                                                                                      gaTCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGc       <  2:812055/139‑1 (MQ=255)

                                                                                                                           ccTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACGt  <  2:488530/140‑1 (MQ=255)

                                                                                                                           ccTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACGt  <  1:370396/140‑1 (MQ=255)

                                                                                                                           ccTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACGt  <  1:329160/140‑1 (MQ=255)

TGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACGT  >  NC_000913/2117456‑2117719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A