Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,230,404	(A)_7→6	intergenic (‑19/‑220)	dusC ← / → yohJ	tRNA‑dihydrouridine(16) synthase/PF03788 family membrane protein YohJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,230,398	0	A	.	100.0%	182.8 / NA	44	intergenic (‑13/‑226)	dusC/yohJ	tRNA‑dihydrouridine(16) synthase/PF03788 family membrane protein YohJ
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (22/22); total (22/22)

AGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGC  >  NC_000913/2230264‑2230533

agcagTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCaaaaaa                                                                                                                                    >  2:215248/1‑140 (MQ=255)

 gcagTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCaaaaaat                                                                                                                                   <  2:649389/140‑2 (MQ=255)

            accacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGACAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATg                                                                                                                       >  2:372592/1‑140 (MQ=255)

                cgcgGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAg                                                                                                                   >  2:75420/1‑140 (MQ=255)

                  cgGACAAACTTGGTTATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAgcg                                                                                                                 <  2:510685/140‑1 (MQ=255)

                          cTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGt                                                                                                         <  1:215248/140‑1 (MQ=255)

                             ggTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAg                                                                                                      <  2:540927/140‑1 (MQ=255)

                             ggTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAg                                                                                                      <  1:418063/140‑1 (MQ=255)

                             ggTGATGCACAGATCGTAGTCGTTAACTTCGGACAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAg                                                                                                      <  1:372592/140‑1 (MQ=255)

                               tGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATg                                                                                                           <  2:283337/133‑1 (MQ=255)

                               tGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATg                                                                                                           >  1:283337/1‑133 (MQ=255)

                                    cacaGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTa                                                                                               >  2:352581/1‑140 (MQ=255)

                                      caGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTaaa                                                                                             <  2:9456/140‑1 (MQ=255)

                                          tCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTc                                                                                         <  1:126139/140‑1 (MQ=255)

                                           cGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCt                                                                                        >  2:417210/1‑140 (MQ=255)

                                              aGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCa                                                                                     >  2:48716/1‑140 (MQ=255)

                                                   ttAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTa                                                                                               <  1:735579/125‑1 (MQ=255)

                                                   ttAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTa                                                                                               >  2:735579/1‑125 (MQ=255)

                                                     aaCTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAggg                                                                              >  1:434102/1‑140 (MQ=255)

                                                     aaCTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAggg                                                                              >  2:459119/1‑140 (MQ=255)

                                                           ggTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgct                                                                        <  1:352581/140‑1 (MQ=255)

                                                                         gCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                          <  2:320050/140‑1 (MQ=255)

                                                                         gCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                          <  1:757472/140‑1 (MQ=255)

                                                                         gCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                          <  1:215350/140‑1 (MQ=255)

                                                                          cACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATccc                                                         <  1:459119/140‑1 (MQ=255)

                                                                           aCCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCct                                                        >  2:222532/1‑140 (MQ=255)

                                                                           aCCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCct                                                        <  1:417210/140‑1 (MQ=255)

                                                                                   gTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGc                                                >  2:29960/1‑140 (MQ=255)

                                                                                       aGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                          >  2:182379/1‑126 (MQ=255)

                                                                                       aGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                          <  1:182379/126‑1 (MQ=255)

                                                                                       aGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCctctct                                                    >  2:187815/1‑132 (MQ=255)

                                                                                       aGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCctctct                                                    <  1:187815/132‑1 (MQ=255)

                                                                                       aGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGa                                            >  2:221004/1‑140 (MQ=255)

                                                                                                tccATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCt                                   <  1:29960/140‑1 (MQ=255)

                                                                                                        tGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGCTTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGc                           <  2:576660/140‑1 (MQ=255)

                                                                                                        tGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGc                           >  1:792125/1‑140 (MQ=255)

                                                                                                          ccAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTg                         >  1:603372/1‑140 (MQ=255)

                                                                                                                 cacacGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCt                          >  2:131737/1‑132 (MQ=255)

                                                                                                                 cacacGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCt                          <  1:131737/132‑1 (MQ=255)

                                                                                                                  acacGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCt                          >  2:44167/1‑131 (MQ=255)

                                                                                                                  acacGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCt                          <  1:44167/131‑1 (MQ=255)

                                                                                                                       cATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAAt            <  1:48716/140‑1 (MQ=255)

                                                                                                                        atatCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATg           >  2:614936/1‑140 (MQ=255)

                                                                                                                               caCCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGa    >  2:495174/1‑140 (MQ=255)

                                                                                                                                aCCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAg   >  2:209799/1‑140 (MQ=255)

                                                                                                                                 cccGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGc  >  2:110358/1‑140 (MQ=255)

AGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGC  >  NC_000913/2230264‑2230533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A