Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	176	31.5	1082462	98.8%	1069472	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,234,242	T→C	G70G (GGT→GGC)	preT →	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,234,242	0	T	C	100.0%	136.9 / NA	38	G70G (GGT→GGC)	preT	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/19); total (19/19)

TGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAAC  >  NC_000913/2234108‑2234367

tGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGt                                                                                                                          <  2:476708/140‑1 (MQ=255)

tGTTTATTATGTCACGACGCTCCCTGTAGTAAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGt                                                                                                                          <  2:330903/140‑1 (MQ=255)

      ttatGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCCATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGc                                                                                                                    <  2:376769/140‑1 (MQ=255)

      ttatGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGc                                                                                                                    <  2:506953/140‑1 (MQ=255)

      ttatGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGc                                                                                                                    <  2:379575/140‑1 (MQ=255)

          gTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCaga                                                                                                                <  1:270397/140‑1 (MQ=255)

            cACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGc                                                                                                                    <  1:209759/134‑1 (MQ=255)

            cACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGc                                                                                                                    >  2:209759/1‑134 (MQ=255)

            cACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAgt                                                                                                              >  2:84863/1‑140 (MQ=255)

            cACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAgt                                                                                                              >  2:500258/1‑140 (MQ=255)

            cACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAgt                                                                                                              >  2:107983/1‑140 (MQ=255)

            cACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAgt                                                                                                              >  1:406378/1‑140 (MQ=255)

                    tCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGa                                                                                                      <  1:500258/140‑1 (MQ=255)

                              cAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAAtt                                                                                            >  1:487748/1‑140 (MQ=255)

                               aGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTa                                                                                           >  2:494281/1‑140 (MQ=255)

                                 gCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATg                                                                                         >  2:453560/1‑140 (MQ=255)

                                  cTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGc                                                                                        <  1:107983/140‑1 (MQ=255)

                                     gCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCaa                                                                                     >  1:35700/1‑140 (MQ=255)

                                      cccGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCaaa                                                                                    <  2:29498/140‑1 (MQ=255)

                                      cccGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCaaa                                                                                    >  2:414962/1‑140 (MQ=255)

                                        cGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGc                                                                                  <  1:313101/140‑1 (MQ=255)

                                               gACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTa                                                                           <  2:406378/140‑1 (MQ=255)

                                                aCCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTAc                                                                          >  2:165161/1‑140 (MQ=255)

                                                aCCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTAc                                                                          >  2:393217/1‑140 (MQ=255)

                                                    aTCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGt                                                                      >  1:336093/1‑140 (MQ=255)

                                                                   ttCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCt                                                       <  2:72306/140‑1 (MQ=255)

                                                                    tCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTc                                                      >  1:235004/1‑140 (MQ=255)

                                                                                     gTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGc                                     <  2:252178/140‑1 (MQ=255)

                                                                                      tAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCt                                    >  1:372348/1‑140 (MQ=255)

                                                                                      tAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCt                                    >  2:356471/1‑140 (MQ=255)

                                                                                      tAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCt                                    >  2:79956/1‑140 (MQ=255)

                                                                                        aTTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTa                                  <  2:35700/140‑1 (MQ=255)

                                                                                               aGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGtt                           <  1:84863/140‑1 (MQ=255)

                                                                                                           gaCAATTCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGAtt               <  1:393217/140‑1 (MQ=255)

                                                                                                                 tCGCGAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGaac         >  1:426550/1‑140 (MQ=255)

                                                                                                                    cgAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGaa          >  2:53037/1‑136 (MQ=255)

                                                                                                                    cgAAAATAATGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGaa          <  1:53037/136‑1 (MQ=255)

                                                                                                                        aataatGCCCTCGGCGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGACCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAAc  <  2:426271/140‑1 (MQ=255)

TGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAAC  >  NC_000913/2234108‑2234367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A