Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,236,296	T→C	C344C (TGT→TGC)	preA →	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,236,296	0	T	C	100.0%	173.5 / NA	50	C344C (TGT→TGC)	preA	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/22); total (28/22)

GGGTTGTCGCATTATCTCGCCGATCAGGGATTTGATTCGCTGCAGGAGATGGTAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGTGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCG  >  NC_000913/2236159‑2236431

gggTTGTCGCATTATCTCGCCGATCAGGGATTTGATTCGCTGCAGGAGATGGTAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATgcgt                                                                                                                                       <  2:481258/140‑1 (MQ=255)

    tGTCGCATTATCTCGCCGATCAGGGATTTGATTCGCTGCAGGAGATGGTAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGc                                                                                                                                   <  1:405412/140‑1 (MQ=255)

                        cAGGGATTTGATTCGCTGCAGGAGATGGTAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGa                                                                                                                             >  1:765716/1‑126 (MQ=255)

                        cAGGGATTTGATTCGCTGCAGGAGATGGTAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGa                                                                                                                             <  2:765716/126‑1 (MQ=255)

                                       cTGCAGGAGATGGTAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGAcggcgg                                                                                                >  2:496882/1‑140 (MQ=255)

                                          cAGGAGATGGTAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTacga                                                                                                      <  1:680106/131‑1 (MQ=255)

                                          cAGGAGATGGTAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTacga                                                                                                      >  2:680106/1‑131 (MQ=255)

                                                    tAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGatg                                                                                   >  2:556019/1‑140 (MQ=255)

                                                    tAGGTCTGACGAATAACAATATTGTCCCGGCGGAAAATTTAGACCGCAGTTATATTGTCTAACACCGTATTAATCTTGATAAATGCGTTGGCTGTGGACCTTGTTATCTTTTCTGTTACGCCGGCGGCCACCAGGCGatg                                                                                   >  2:392797/1‑140 (MQ=255)

                                                         cTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGatggaatg                                                                              >  2:704528/1‑140 (MQ=255)

                                                          tGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGatggaatgg                                                                             >  1:820088/1‑140 (MQ=255)

                                                           ggCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTGTATTTCCTGTTACGACGGCGGTCACCAGGCGatggaatgga                                                                            >  1:311660/1‑140 (MQ=255)

                                                           ggCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGatggaatgga                                                                            >  2:599416/1‑140 (MQ=255)

                                                            gCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAg                                                                           >  1:433729/1‑140 (MQ=255)

                                                              gAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCg                                                                         >  2:68902/1‑140 (MQ=255)

                                                                aTAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCgag                                                                       <  1:68902/140‑1 (MQ=255)

                                                                          tGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGca                                                             <  1:599416/140‑1 (MQ=255)

                                                                            tCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGcaca                                                           >  2:287368/1‑140 (MQ=255)

                                                                            tCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGcaca                                                           >  1:87199/1‑140 (MQ=255)

                                                                            tCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGcaca                                                           >  2:783763/1‑140 (MQ=255)

                                                                            tCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGcaca                                                           >  2:819679/1‑140 (MQ=255)

                                                                             cccGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCacac                                                          <  1:704528/140‑1 (MQ=255)

                                                                               cggcggAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTGTATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCg                                                        <  2:311660/140‑1 (MQ=255)

                                                                               cggcggAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCg                                                        <  2:402561/140‑1 (MQ=255)

                                                                               cggcggAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCg                                                        <  2:433729/140‑1 (MQ=255)

                                                                               cggcggAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCg                                                        <  2:87199/140‑1 (MQ=255)

                                                                               cggcggAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCg                                                        <  1:819679/140‑1 (MQ=255)

                                                                               cggcggAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACAGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCg                                                        >  2:663153/1‑140 (MQ=255)

                                                                                 gcggAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCa                                                      >  2:669623/1‑140 (MQ=255)

                                                                                 gcggAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCa                                                      <  1:783763/140‑1 (MQ=255)

                                                                                      aGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCg                                                        >  2:440818/1‑133 (MQ=255)

                                                                                      aGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCg                                                        <  1:440818/133‑1 (MQ=255)

                                                                                        aTTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAAt                                               <  1:287368/140‑1 (MQ=255)

                                                                                          ttAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATAc                                             <  2:820088/140‑1 (MQ=255)

                                                                                              aCCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCgag                                         <  1:392797/140‑1 (MQ=255)

                                                                                                 gCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGaaa                                      >  1:309082/1‑140 (MQ=255)

                                                                                                  cAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAAt                                     >  1:309345/1‑140 (MQ=255)

                                                                                                               tATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGt                          <  1:367386/138‑1 (MQ=255)

                                                                                                               tATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGt                          >  2:367386/1‑138 (MQ=255)

                                                                                                               tATCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCt                        >  2:54100/1‑140 (MQ=255)

                                                                                                                aTCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTg                       <  1:54100/140‑1 (MQ=255)

                                                                                                                 tCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGc                      >  1:100421/1‑140 (MQ=255)

                                                                                                                 tCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGc                      >  1:356677/1‑140 (MQ=255)

                                                                                                                 tCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGc                      >  1:829776/1‑140 (MQ=255)

                                                                                                                 tCCCCGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGc                      >  1:814020/1‑140 (MQ=255)

                                                                                                                  ccccGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCt                     >  2:169694/1‑140 (MQ=255)

                                                                                                                  ccccGTATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACAGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCt                     <  1:663153/140‑1 (MQ=255)

                                                                                                                       tATCAATCTTGATAAATGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTtgtg                >  1:728632/1‑140 (MQ=255)

                                                                                                                       tATCAATCTTGATAAATGCGATGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTtgtg                >  2:575511/1‑140 (MQ=255)

                                                                                                                         tCAATCTTGATAAATGCGATGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGt              <  1:575511/140‑1 (MQ=255)

                                                                                                                                     aaTGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCg  <  1:620393/140‑1 (MQ=255)

                                                                                                                                     aaTGCGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCg  <  2:402406/140‑1 (MQ=255)

GGGTTGTCGCATTATCTCGCCGATCAGGGATTTGATTCGCTGCAGGAGATGGTAGGTCTGGCGAATAACAATATTGTCCCGGCGGAAGATTTAGACCGCAGTTATATTGTCTATCCCCGTATCAATCTTGATAAATGTGTTGGCTGTGGACGCTGTTATATTTCCTGTTACGACGGCGGTCACCAGGCGATGGAATGGAGCGAGAAAACCCGCACACCGCATTGTAATACCGAGAAATGTGTGGGTTGTCTGCTTTGTGGTCACGTCTGCCCG  >  NC_000913/2236159‑2236431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A