Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,305,788	T→C	M323V (ATG→GTG)	mqo ←	malate:quinone oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,305,788	0	T	C	100.0%	180.7 / NA	52	M323V (ATG→GTG)	mqo	malate:quinone oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/27); total (25/27)

GGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCGTAGTCTTTCGCTTCCGGAA  >  NC_000913/2305660‑2305913

ggtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTgcgc                                                                                                                    >  1:611071/1‑140 (MQ=255)

 gtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGcc                                                                                                                   >  1:237435/1‑140 (MQ=255)

 gtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGcc                                                                                                                   >  1:201448/1‑140 (MQ=255)

 gtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGcc                                                                                                                   >  2:591959/1‑140 (MQ=255)

 gtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGcc                                                                                                                   >  2:342695/1‑140 (MQ=255)

   ggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCaa                                                                                                                 >  2:579404/1‑140 (MQ=255)

    gtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAAc                                                                                                                <  2:637637/140‑1 (MQ=255)

    gtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAAc                                                                                                                <  2:237435/140‑1 (MQ=255)

     tggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACg                                                                                                               >  2:407615/1‑140 (MQ=255)

      ggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACgg                                                                                                              <  1:579404/140‑1 (MQ=255)

       gAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGa                                                                                                             <  1:407615/140‑1 (MQ=255)

        aaCTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGAt                                                                                                            >  1:702634/1‑140 (MQ=255)

            cATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCtt                                                                                                        >  2:98569/1‑140 (MQ=255)

             aTTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCt                                                                                                         <  2:91303/138‑1 (MQ=255)

             aTTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCt                                                                                                         >  1:91303/1‑138 (MQ=255)

                  aTCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCtt                                                                                                        >  1:587689/1‑134 (MQ=255)

                  aTCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCtt                                                                                                        <  2:587689/134‑1 (MQ=255)

                      cacaATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAAc                                                                                              <  1:98569/140‑1 (MQ=255)

                       acaATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACTCCGGTGGTGCGCCAACGGATGCTTTACCGTAAAcc                                                                                             >  1:46506/1‑140 (MQ=255)

                       acaATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAAcc                                                                                             >  1:393185/1‑140 (MQ=255)

                       acaATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAAcc                                                                                             >  2:429681/1‑140 (MQ=255)

                             aaCCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGcc                                                                                       >  1:730253/1‑140 (MQ=255)

                                cGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACTCCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                                                                                    <  2:46506/140‑1 (MQ=255)

                                cGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                                                                                    >  2:767456/1‑140 (MQ=255)

                                cGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                                                                                    >  2:51755/1‑140 (MQ=255)

                                cGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                                                                                    <  2:292463/140‑1 (MQ=255)

                                cGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACACGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                                                                                    >  2:653486/1‑140 (MQ=255)

                                 gTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGAt                                                                                   <  1:566910/140‑1 (MQ=255)

                                 gTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGAt                                                                                   <  2:631747/140‑1 (MQ=255)

                                  tttttGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                                                                                    <  2:676520/138‑1 (MQ=255)

                                  tttttGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                                                                                    >  1:468041/1‑138 (MQ=255)

                                  tttttGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                                                                                    <  2:468041/138‑1 (MQ=255)

                                  tttttGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGa                                                                                    >  1:676520/1‑138 (MQ=255)

                                  tttttGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATg                                                                                  <  2:817446/140‑1 (MQ=255)

                                   ttttGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATgg                                                                                 >  1:469358/1‑140 (MQ=255)

                                           aaTTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAAc                                                                         <  2:469358/140‑1 (MQ=255)

                                             tttGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCa                                                                       <  2:730253/140‑1 (MQ=255)

                                                           tGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTcc                                                         >  2:444806/1‑140 (MQ=255)

                                                            ggCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCg                                                        <  1:591959/140‑1 (MQ=255)

                                                            ggCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCg                                                        <  1:51755/140‑1 (MQ=255)

                                                            ggCAAATGGCCCAAACAGCACTACACGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCg                                                        <  1:653486/140‑1 (MQ=255)

                                                                 aTGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACa                                                   <  2:201448/140‑1 (MQ=255)

                                                                     cccAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGa                                               <  1:767456/140‑1 (MQ=255)

                                                                                   cgcgTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCAcc                                 >  1:325821/1‑140 (MQ=255)

                                                                                               ccAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCg                     <  1:89856/140‑1 (MQ=255)

                                                                                               ccAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCg                     <  1:444806/140‑1 (MQ=255)

                                                                                                cAGAACGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCGt                    <  1:819332/140‑1 (MQ=255)

                                                                                                   aaCGCGGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCGTAGt                 >  2:158207/1‑140 (MQ=255)

                                                                                                     cgcgGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCGTAGTCt               >  2:28008/1‑140 (MQ=255)

                                                                                                       cgGGTATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCGTAGTCttt             <  2:611071/140‑1 (MQ=255)

                                                                                                           tATCGATATGCGGAACCGACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCGTAGTCTTTCGCt         <  2:325821/140‑1 (MQ=255)

                                                                                                                         aCCTACACCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCGTAGTCTTTCGCTTCCGGaa  <  2:197447/133‑1 (MQ=255)

GGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCACTACGCGTTTACCGTCCAGAACGCGGGTATCGATATGCGGAACCGACATCGGTGGTGCGCCAACGGATGCTTTACCGTAAACCTTCGCCAGATGGTGATTAACCACGTCCGGGTTTTCCGAAACAAGGAACTGTCCGCCCACCGGGAAACCGGCGTAGTCTTTCGCTTCCGGAA  >  NC_000913/2305660‑2305913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A