Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F28 I1 R1
|
252 |
48.5 |
1661298 |
98.8% |
1641362 |
138.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,177,509 |
(C)6→7 |
coding (402/630 nt) |
nudF ← |
ADP‑sugar pyrophosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,177,503 | 1 | . | C | 89.7%
| 117.1
/ 7.6
| 39 | coding (408/630 nt) | nudF | ADP‑sugar pyrophosphatase |
| Reads supporting (aligned to +/- strand): ref base . (2/2); new base C (20/15); total (22/17) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 |
TCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑G‑‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAA > NC_000913/3177369‑3177627
|
tctACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑G‑‑ccccc < 1:733289/140‑1 (MQ=255)
aCTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCt < 1:661258/140‑1 (MQ=255)
tGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTg > 1:132847/1‑140 (MQ=255)
tGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTg > 1:488070/1‑140 (MQ=255)
tGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTg < 1:274555/140‑1 (MQ=255)
gTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑G‑C‑‑CCCCCCGGGCTTGCCAGGAAAC‑tt > 1:180190/1‑140 (MQ=255)
cccGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTaa > 1:322943/1‑140 (MQ=255)
cGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAaca < 2:322943/140‑1 (MQ=255)
cGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAaca < 2:132847/140‑1 (MQ=255)
cGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAaca < 1:575835/140‑1 (MQ=255)
ggCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑G‑‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACAcc > 1:372552/1‑140 (MQ=255)
aCCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGtt > 2:402159/1‑140 (MQ=255)
ccACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑G‑C‑‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTg < 2:180190/140‑1 (MQ=255)
cacaTGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGaaa > 2:741178/1‑125 (MQ=255)
cacaTGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGaaa < 1:741178/125‑1 (MQ=255)
caTGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGt < 1:662609/140‑1 (MQ=255)
aTGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑G‑‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTcc < 2:372552/140‑1 (MQ=255)
aaCGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑Gt > 1:89814/1‑140 (MQ=255)
aaCGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑Gt < 1:530550/140‑1 (MQ=255)
gcgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GCCCCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑Gttt < 1:690997/140‑1 (MQ=255)
gcgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTg < 1:485836/140‑1 (MQ=255)
gcgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTg < 2:180651/140‑1 (MQ=255)
gcgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTg < 1:402159/140‑1 (MQ=255)
cgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGa > 1:189119/1‑140 (MQ=255)
cgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGa > 1:1683/1‑140 (MQ=255)
gAATATCTTCGTTTTCATCAGCCAGACCGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGAc < 2:189119/140‑1 (MQ=255)
gAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGAc > 1:190725/1‑140 (MQ=255)
tttttCATCAGCCAGACCGTGAATCCCGCTTGCGGTCGGGGCGCCCCCTCCCCCCACCATAATTGACGACCGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACCCCGGTTTGGTCC‑GTTTGACTATCAGTccc < 2:488070/139‑1 (MQ=255)
tGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTcgc > 1:48460/1‑140 (MQ=255)
aaTACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGa > 1:251698/1‑140 (MQ=255)
ttGCGGTCGTGGCGTCCACTCCCCCCACCATAATTGACGACCGCTCACTGG‑TCC‑‑CCCCCCGGGCTTGCCAGAAAAC‑TTAACCCCGGTTTGGCCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAAc < 2:587136/140‑1 (MQ=255)
tGCGGTCGTGGCGTCCACTTCGCCCACCATAATTTACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACa > 2:155955/1‑140 (MQ=255)
gCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACAt > 1:350272/1‑140 (MQ=255)
gTGGCGCCCTCTTCCCCCACAATAATTGACGAACGCTCACTGG‑TCC‑‑CCCCCCGGGCTTGCCAGGAAAC‑TTACCCCCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCca < 2:48460/140‑1 (MQ=255)
gcctccccccACAAAATTTGACGACCGCTCACTGG‑G‑C‑‑CCCCCCGGGGTTTCCCAGAAACTTTAACACCGG‑TTGGTCCTGTTTAACTACCGGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCa < 2:358991/133‑1 (MQ=255)
cccTTCCCCCCCCATAATTGACGAACGCTCACTGGCC‑C‑‑CCCCCCGGGCTTCCCAGGAACC‑TCCACCCCGTTTTGGTCG‑GTTTGCCTATCAGCCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCa < 2:143434/138‑1 (MQ=255)
ccccACCATAATTTACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCctct < 1:155955/139‑1 (MQ=255)
ccccACCATAATTGACGAACGCTCACTGG‑TCC‑‑CCCCCCGGGCTTGCCAGAAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCctct < 2:89814/139‑1 (MQ=255)
accaTAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCaa > 2:50247/1‑140 (MQ=255)
ccaTAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAat > 1:246541/1‑140 (MQ=255)
ccaTAATTGACAACCGCTCACTGG‑TCC‑‑CCCCCCGGGCTTGCAAAGAATC‑TTAACACCGTTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAat < 2:251698/140‑1 (MQ=255)
aTAATTGACGAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAatct > 1:358991/1‑139 (MQ=255)
gaccGCCCACTGG‑TCC‑‑CCCCCCGGGTTTGCCAGGAAAC‑TTACCCCCGTTTTGGTCC‑TTTTGACTACCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCaa < 2:189246/137‑1 (MQ=255)
gAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGTCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCaa > 1:699722/1‑140 (MQ=255)
gAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCaa > 1:779986/1‑140 (MQ=255)
gAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCaa > 1:601048/1‑140 (MQ=255)
gAACGCTCACTGG‑T‑GC‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCaa > 1:189246/1‑140 (MQ=255)
|
TCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGG‑T‑G‑‑CCCCCCGGGCTTGCCAGGAAAC‑TTAACACCGGTTTGGTCC‑GTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAA > NC_000913/3177369‑3177627
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
