Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 164 66.7 2276140 99.1% 2255654 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,177,829 (A)7→6 coding (82/630 nt) nudF ← ADP‑sugar pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,177,8230A.100.0% 229.8 / NA 54coding (88/630 nt)nudFADP‑sugar pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (25/29);  total (25/29)

AGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGAC  >  NC_000913/3177690‑3177958
                                                                                                                                     |                                                                                                                                       
aGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGGGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaaaag                                                                                                                                   <  1:467000/140‑2 (MQ=255)
       cTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGAt                                                                                                                           <  1:512863/140‑1 (MQ=255)
            tcaCGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAgt                                                                                                                      >  1:424126/1‑140 (MQ=255)
            tcaCGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAgt                                                                                                                      >  1:276059/1‑140 (MQ=255)
                 cACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTtc                                                                                                                 <  1:249281/140‑1 (MQ=255)
                  ccTGGGTCAAAGGGTAGCAAGCCTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTtct                                                                                                                <  2:1083458/139‑1 (MQ=255)
                  aCTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTtct                                                                                                                >  1:883061/1‑140 (MQ=255)
                     gggTCAAAGGGTAGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGt                                                                                                             <  2:1023288/140‑1 (MQ=255)
                     gggTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTtctctg                                                                                                             >  1:287762/1‑138 (MQ=255)
                     gggTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGt                                                                                                             >  2:32187/1‑140 (MQ=255)
                      ggTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCGTTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTg                                                                                                            >  2:1079055/1‑140 (MQ=255)
                       gTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGc                                                                                                           >  2:403433/1‑140 (MQ=255)
                        tCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCa                                                                                                          <  2:276059/140‑1 (MQ=255)
                          aaggggTAGCAAGCCTGCGGCGTGCCCGCGCTAAAAATTTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCaat                                                                                                        <  2:981666/137‑1 (MQ=255)
                             gggTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCaataat                                                                                                     <  2:424126/140‑1 (MQ=255)
                                 aGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCt                                                                                                 >  1:439349/1‑140 (MQ=255)
                                        cTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGt                                                                                          >  1:770178/1‑140 (MQ=255)
                                          gcggcgTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGttt                                                                                        >  2:698825/1‑140 (MQ=255)
                                           cggcgTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGtttt                                                                                       >  1:89129/1‑140 (MQ=255)
                                                gcaCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCa                                                                                  <  2:770178/138‑1 (MQ=255)
                                                       gcTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTaa                                                                           <  1:506105/140‑1 (MQ=255)
                                                           aaaaaTTTCCCGCCGCACCTCATGACTCGTTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACggg                                                                       <  1:1079055/140‑1 (MQ=255)
                                                                           aCCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTa                                                       <  2:77280/140‑1 (MQ=255)
                                                                            ccTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTaa                                                      <  2:916568/140‑1 (MQ=255)
                                                                             cTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAg                                                     <  2:883061/140‑1 (MQ=255)
                                                                             cTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAg                                                     <  2:986606/140‑1 (MQ=255)
                                                                             cTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAg                                                     <  1:17886/140‑1 (MQ=255)
                                                                             cTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAg                                                     <  1:245315/140‑1 (MQ=255)
                                                                             cTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAg                                                     <  1:698825/140‑1 (MQ=255)
                                                                               cATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCa                                                   <  1:586901/140‑1 (MQ=255)
                                                                                     tCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTa                                             >  1:421687/1‑140 (MQ=255)
                                                                                       aTTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAg                                                     <  1:274315/130‑1 (MQ=255)
                                                                                       aTTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAg                                                     >  2:274315/1‑130 (MQ=255)
                                                                                       aTTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAAt                                           >  2:419916/1‑140 (MQ=255)
                                                                                         ttGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGt                                         >  1:503888/1‑140 (MQ=255)
                                                                                          tGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTc                                        <  2:503888/140‑1 (MQ=255)
                                                                                          tGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTc                                        <  1:32187/140‑1 (MQ=255)
                                                                                          tGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTc                                        <  2:421687/140‑1 (MQ=255)
                                                                                            cccGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCt                                      <  1:254787/140‑1 (MQ=255)
                                                                                            cccGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCt                                      <  1:728574/140‑1 (MQ=255)
                                                                                            cccGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCt                                      >  2:254787/1‑140 (MQ=255)
                                                                                                   aaTAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAAt                                           <  1:1042733/128‑1 (MQ=255)
                                                                                                   aaTAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAAt                                           >  2:1042733/1‑128 (MQ=255)
                                                                                                      aGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATa                            <  2:89129/140‑1 (MQ=255)
                                                                                                       gACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAg                           <  2:355618/140‑1 (MQ=255)
                                                                                                       gACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAg                           <  2:902310/140‑1 (MQ=255)
                                                                                                             gACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCt                                      <  2:18816/123‑1 (MQ=255)
                                                                                                             gACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCt                                      >  1:18816/1‑123 (MQ=255)
                                                                                                             gACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAAtt                     >  2:643923/1‑140 (MQ=255)
                                                                                                                 aaaTCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAAt                 >  2:1046898/1‑140 (MQ=255)
                                                                                                                  aaTCTATAAAGATCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAAtg                >  1:878397/1‑140 (MQ=255)
                                                                                                                              tCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCg    >  2:1044007/1‑140 (MQ=255)
                                                                                                                              tCTAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCg    >  1:995560/1‑140 (MQ=255)
                                                                                                                                tAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGAc  >  1:868610/1‑140 (MQ=255)
                                                                                                                                tAATG‑AAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGAc  >  1:1122295/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                       
AGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGAC  >  NC_000913/3177690‑3177958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A