Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,082,980	G→A	R75R (CGG→CGA)	sbcB →	exodeoxyribonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,082,980	0	G	A	100.0%	131.9 / NA	38	R75R (CGG→CGA)	sbcB	exodeoxyribonuclease I
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/14); total (24/14)

CGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTT  >  NC_000913/2082848‑2083114

cGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAg                                                                                                                                 >  2:80249/1‑140 (MQ=255)

                 aTAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCcgcg                                                                                                                >  1:857151/1‑140 (MQ=255)

                      gAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTCTgc                                                                                                           <  1:467664/140‑1 (MQ=255)

                       aaTTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTgcc                                                                                                          >  2:74583/1‑140 (MQ=255)

                         ttCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTgccgc                                                                                                        <  1:896400/140‑1 (MQ=255)

                               gTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTAt                                                                                                  >  1:385077/1‑140 (MQ=255)

                                  aTCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCa                                                                                               >  1:135055/1‑140 (MQ=255)

                                  aTCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCa                                                                                               >  1:867359/1‑140 (MQ=255)

                                  aTCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCACCCGTATTCa                                                                                               >  2:813395/1‑140 (MQ=255)

                                          aCCCGAAGTCTTTTACTGCAAGCCTGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCttt                                                                                       >  2:913306/1‑140 (MQ=255)

                                          aCCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCttt                                                                                       >  2:57938/1‑140 (MQ=255)

                                           cccGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCtttt                                                                                      <  1:80249/140‑1 (MQ=255)

                                               aaGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCACCCGTATTCACTCGCTTTTtacc                                                                                  <  1:813395/140‑1 (MQ=255)

                                                       tACTGCAAGCCTGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGaa                                                                          <  1:913306/140‑1 (MQ=255)

                                                            cAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCt                                                                     >  2:970671/1‑140 (MQ=255)

                                                                  cGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTc                                                               <  1:495798/140‑1 (MQ=255)

                                                                         gaCTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTa                                                        >  1:1022570/1‑140 (MQ=255)

                                                                                      agccagGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGt                                           >  1:103184/1‑140 (MQ=255)

                                                                                      agccagGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGt                                           >  2:589057/1‑140 (MQ=255)

                                                                                      agccagGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGt                                           >  2:448142/1‑140 (MQ=255)

                                                                                        ccagGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGttt                                         >  1:56287/1‑140 (MQ=255)

                                                                                              gCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacga                                   <  1:589057/140‑1 (MQ=255)

                                                                                              gCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacga                                   <  1:448142/140‑1 (MQ=255)

                                                                                                  tATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacgacg                                 >  1:356300/1‑138 (MQ=255)

                                                                                                  tATTAATTACCGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacgacg                                 <  2:356300/138‑1 (MQ=255)

                                                                                                           ccGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACg                       >  2:532258/1‑139 (MQ=255)

                                                                                                           ccGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACg                       <  1:532258/139‑1 (MQ=255)

                                                                                                            cGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacga                                   <  2:1032470/126‑1 (MQ=255)

                                                                                                            cGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacga                                   <  2:112720/126‑1 (MQ=255)

                                                                                                            cGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacga                                   >  1:112720/1‑126 (MQ=255)

                                                                                                            cGGTATTACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacga                                   >  1:1032470/1‑126 (MQ=255)

                                                                                                                 ttACCCCGCAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAtt                >  1:742395/1‑140 (MQ=255)

                                                                                                                         cAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCg        >  1:370709/1‑140 (MQ=255)

                                                                                                                         cAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCg        >  1:134382/1‑140 (MQ=255)

                                                                                                                         cAGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCg        >  1:1067215/1‑140 (MQ=255)

                                                                                                                          aGGAAGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCCGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGt       <  2:135055/140‑1 (MQ=255)

                                                                                                                             aaGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAt    <  2:385077/140‑1 (MQ=255)

                                                                                                                              aGCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAtt   >  1:19555/1‑140 (MQ=255)

                                                                                                                               gCACGAGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAttt  >  2:476398/1‑140 (MQ=255)

CGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTT  >  NC_000913/2082848‑2083114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A