Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 164 66.7 2276140 99.1% 2255654 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,471,866 A→G pseudogene (197/519 nt) yfdL ← CPS‑53 (KpLE1) prophage; putative uncharacterized protein YfdL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,471,8660AG100.0% 216.0 / NA 57pseudogene (197/519 nt)yfdLCPS‑53 (KpLE1) prophage; putative uncharacterized protein YfdL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/25);  total (32/25)

TGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCGCGGTAAGCAGCTGGTCAT  >  NC_000913/2471732‑2471998
                                                                                                                                      |                                                                                                                                    
tgtCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCAttt                                                                                                                                 <  1:711271/140‑1 (MQ=255)
  tCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCAt                                                                                                                                   <  2:489891/136‑1 (MQ=255)
  tCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCAt                                                                                                                                   >  1:489891/1‑136 (MQ=255)
  tCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCt                                                                                                                               >  2:8424/1‑140 (MQ=255)
   ccTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCtt                                                                                                                              >  2:965426/1‑140 (MQ=255)
        gaCGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGc                                                                                                                         >  2:51302/1‑140 (MQ=255)
           gTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGc                                                                                                                      <  2:963856/140‑1 (MQ=255)
            tCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCt                                                                                                                     <  2:517548/140‑1 (MQ=255)
            tCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCt                                                                                                                     >  1:987366/1‑140 (MQ=255)
            tCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCt                                                                                                                     >  1:542168/1‑140 (MQ=255)
            tCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCt                                                                                                                     >  2:44874/1‑140 (MQ=255)
            tCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCt                                                                                                                     >  2:282915/1‑140 (MQ=255)
                       tCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAAcc                                                                                                          >  1:348833/1‑140 (MQ=255)
                        cAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCa                                                                                                         >  1:174084/1‑140 (MQ=255)
                                  ccATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCga                                                                                               <  2:542168/140‑1 (MQ=255)
                                    aTTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCgaga                                                                                             <  1:282915/140‑1 (MQ=255)
                                             ttAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGc                                                                                    >  2:248252/1‑140 (MQ=255)
                                              tAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCa                                                                                   <  2:372818/140‑1 (MQ=255)
                                                  ccGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGt                                                                               >  1:919820/1‑140 (MQ=255)
                                                    ggATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGc                                                                             >  2:956299/1‑140 (MQ=255)
                                                     gATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGcc                                                                            >  1:496115/1‑140 (MQ=255)
                                                      aTGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCg                                                                           <  1:248252/140‑1 (MQ=255)
                                                        ggCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTc                                                                         <  1:871264/140‑1 (MQ=255)
                                                        ggCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTc                                                                         <  1:206436/140‑1 (MQ=255)
                                                          cAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCa                                                                       <  1:580799/140‑1 (MQ=255)
                                                                ttGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGa                                                                 <  2:919820/140‑1 (MQ=255)
                                                                    tatCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATc                                                             >  2:235870/1‑140 (MQ=255)
                                                                       ccAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGcc                                                          <  1:51302/140‑1 (MQ=255)
                                                                         aGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCaa                                                        <  1:487886/140‑1 (MQ=255)
                                                                                 aaGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTc                                                >  1:55216/1‑140 (MQ=255)
                                                                                 aaGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGCTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTc                                                >  2:980481/1‑140 (MQ=255)
                                                                                  aGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCt                                               <  2:174084/140‑1 (MQ=255)
                                                                                  aGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCt                                               <  1:44874/140‑1 (MQ=255)
                                                                                     aGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGc                                            <  2:348833/140‑1 (MQ=255)
                                                                                      gAAAGCATAAAACTTCCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCg                                           >  2:1070059/1‑140 (MQ=255)
                                                                                       aaaGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCgg                                          <  1:913735/140‑1 (MQ=255)
                                                                                                   tACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGc                                            >  2:28475/1‑126 (MQ=255)
                                                                                                   tACCCATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGc                                            <  1:28475/126‑1 (MQ=255)
                                                                                                     cccATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAgcgc                            >  2:1137955/1‑140 (MQ=255)
                                                                                                     cccATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAgcgc                            >  1:65751/1‑140 (MQ=255)
                                                                                                     cccATATCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAgcgc                            >  2:968435/1‑140 (MQ=255)
                                                                                                          atCAGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGaa                       <  1:8424/140‑1 (MQ=255)
                                                                                                             aGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAgcg                    <  1:980481/140‑1 (MQ=255)
                                                                                                             aGGTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAgcg                    <  1:319046/140‑1 (MQ=255)
                                                                                                               gTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGcgcg                  >  2:973647/1‑140 (MQ=255)
                                                                                                               gTATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGcgcg                  <  2:987366/140‑1 (MQ=255)
                                                                                                                tATCTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCGCgg                 <  2:496115/140‑1 (MQ=255)
                                                                                                                   cTGATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCGCGGTaa              >  2:804268/1‑140 (MQ=255)
                                                                                                                     gATTCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCTAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCGCGGTAagc            >  1:183795/1‑140 (MQ=255)
                                                                                                                       ttCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCGCGGTAagcag          >  1:768627/1‑140 (MQ=255)
                                                                                                                       ttCGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCGCGGTAagcag          >  1:1125162/1‑140 (MQ=255)
                                                                                                                         cGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAgcg                    <  2:134105/128‑1 (MQ=255)
                                                                                                                         cGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAgcg                    >  1:134105/1‑128 (MQ=255)
                                                                                                                         cGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCGCGGTAAGCAGCt        >  2:902426/1‑140 (MQ=255)
                                                                                                                         cGCCCCTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCGCGGTAAGCAGCt        >  1:310155/1‑140 (MQ=255)
                                                                                                                              cTGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCGCGGTAAGCAGCTGGTCa   >  2:336706/1‑140 (MQ=255)
                                                                                                                               tGTCCCTGCATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCGCGGTAAGCAGCTGGTCAt  >  1:30950/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                    
TGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGGTTTTCGAGAGCCTTTTGCACCGTGCCGTCCAATTTGATATCGCCAAACGGATTCTTGCGGCTTAACAGCAGCGCACGAAGCGCGGTAAGCAGCTGGTCAT  >  NC_000913/2471732‑2471998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A