Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 164 66.7 2276140 99.1% 2255654 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,693,457 G→A A696V (GCG→GTG)  purL ← phosphoribosylformylglycinamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,693,4570GA100.0% 107.0 / NA 34A696V (GCG→GTG) purLphosphoribosylformylglycinamide synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/20);  total (14/20)

GTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGCCCCACCATCTGATCGCGCGCTACCATGCCGGTTACGCTGCGGT  >  NC_000913/2693327‑2693574
                                                                                                                                  |                                                                                                                     
gTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGttt                                                                                                              >  1:845685/1‑140 (MQ=255)
 ttGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTc                                                                                                             >  2:256695/1‑140 (MQ=255)
 ttGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTc                                                                                                             >  1:480100/1‑140 (MQ=255)
      ggAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCgt                                                                                                        >  1:546118/1‑140 (MQ=255)
           gTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGTTTCACCGtagtag                                                                                                   <  2:845685/140‑1 (MQ=255)
                  gCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGa                                                                                            >  2:233551/1‑140 (MQ=255)
                   cGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAg                                                                                           <  2:317443/140‑1 (MQ=255)
                      ttGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCt                                                                                        >  2:436145/1‑140 (MQ=255)
                             cGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTa                                                                                 >  2:1079920/1‑140 (MQ=255)
                                  aTTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGAc                                                                            <  2:546118/140‑1 (MQ=255)
                                    ttGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACcg                                                                          <  1:436145/140‑1 (MQ=255)
                                    ttGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACcg                                                                          >  2:1087032/1‑140 (MQ=255)
                                          tGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGt                                                                    <  1:1087032/140‑1 (MQ=255)
                                            cggcgATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTa                                                                  >  2:20706/1‑140 (MQ=255)
                                                 aTGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGAc                                                             >  1:424368/1‑140 (MQ=255)
                                                        ttAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCAcc                                                      <  1:256695/140‑1 (MQ=255)
                                                             gCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCa                                                 <  1:233551/140‑1 (MQ=255)
                                                             gCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCa                                                 <  1:108872/140‑1 (MQ=255)
                                                              cTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCAc                                                <  2:697948/140‑1 (MQ=255)
                                                               ttCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACg                                               <  2:461651/140‑1 (MQ=255)
                                                                 cACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGc                                             <  1:1075803/140‑1 (MQ=255)
                                                                   ccgaccgCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGccc                                           <  1:569339/140‑1 (MQ=255)
                                                                   ccgaccgCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGccc                                           <  1:462561/140‑1 (MQ=255)
                                                                      accgCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGCCccac                                        >  2:510039/1‑140 (MQ=255)
                                                                         gCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGCCCCACCAt                                     >  2:516251/1‑140 (MQ=255)
                                                                              aCGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGCCCCACCATCTGAt                                <  1:1079920/140‑1 (MQ=255)
                                                                                  gCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGCCCCACCATCTGATcgcg                            <  1:554469/140‑1 (MQ=255)
                                                                                           gcgAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGCCCCACCATCTGATCGCGCGCTACCAt                   <  2:1134054/140‑1 (MQ=255)
                                                                                                     gcagcGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGCCCCACCATCTGATCGCGCGCTACCATGCCGGTTACg         <  1:372861/140‑1 (MQ=255)
                                                                                                      cagcGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGCCCCACCATCTGATCGCGCGCTACCATGCCGGTTACGc        >  2:1111257/1‑140 (MQ=255)
                                                                                                       agcGCAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGCCCCACCATCTGATCGCGCGCTACCATGCCGGTTACGCt       <  2:424368/140‑1 (MQ=255)
                                                                                                          gcAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGCCCCACCATCTGATCGCGCGCTACCATGCCGGTTACGCTGCg    <  1:188359/140‑1 (MQ=255)
                                                                                                           cAACCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGCCCCACCATCTGATCGCGCGCTACCATGCCGGTTACGCTGCgg   >  2:636461/1‑140 (MQ=255)
                                                                                                            aaCCGGCGCACGCTCGCCAATCACCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGCCCCACCATCTGATTGCGCGCTACCATGCCGGTTACGCTGCGGt  <  1:1015070/140‑1 (MQ=255)
                                                                                                                                  |                                                                                                                     
GTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGCCCCACCATCTGATCGCGCGCTACCATGCCGGTTACGCTGCGGT  >  NC_000913/2693327‑2693574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A