Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,014,973	(C)_6→7	coding (85/771 nt)	yqeC ←	uncharacterized protein YqeC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,014,967	1	.	C	97.5%	291.8 / ‑0.5	80	coding (91/771 nt)	yqeC	uncharacterized protein YqeC
Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (40/38); total (41/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTT‑‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCCCAACTTAATC  >  NC_000913/3014829‑3015101

gAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTT‑‑c                                                                                                                                       <  2:713377/140‑1 (MQ=255)

    aaGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTT‑‑ccccc                                                                                                                                   <  1:366032/140‑1 (MQ=255)

     aGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCCCTAGCCTGGGGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTT‑‑cccccc                                                                                                                                  >  2:1024390/1‑140 (MQ=255)

         tGGCGGGATCACGACAGAAAACTACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTT‑‑cccccccagc                                                                                                                              <  1:804170/140‑5 (MQ=255)

          ggCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTT‑‑cccccccagca                                                                                                                             >  2:29885/1‑135 (MQ=255)

           gCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCC‑AAAAAGCAAGCTGGTTTTC‑CCCCCCAGCAcc                                                                                                                            <  2:837285/140‑1 (MQ=255)

            cGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTT‑‑cccccc                                                                                                                                  <  1:103966/133‑1 (MQ=255)

            cGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTT‑‑cccccc                                                                                                                                  >  2:103966/1‑133 (MQ=255)

            cGGGATCACGACAGAAAACCACGGGCCAGGGAGACGTTGGCATAAACATATGTGTCGTCGTCGGGATTAACACACGCCCGCCGCGAGCCTGGAGCAGTTCTTCCAGCCAAAAAAGCAAACTGGTTTTC‑CCCCCCAGCAcc                                                                                                                            >  2:768355/1‑140 (MQ=255)

                aTCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACcaaca                                                                                                                        <  1:996903/140‑1 (MQ=255)

                 tCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACcaacaa                                                                                                                       >  1:535105/1‑140 (MQ=255)

                     gACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGa                                                                                                                   <  1:913914/140‑1 (MQ=255)

                          aaaaCCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCATCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCCAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATca                                                                                                              <  2:780890/140‑1 (MQ=255)

                                 cGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTggg                                                                                                       >  1:92259/1‑140 (MQ=255)

                                  gggCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGa                                                                                                      <  2:92259/140‑1 (MQ=255)

                                        gTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTc                                                                                                >  1:581653/1‑140 (MQ=255)

                                          gagaCGTTGGCATAAACATATGGGTCGTCGTCGTGATTACCACCCGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTg                                                                                              <  2:1056954/140‑1 (MQ=255)

                                           ggaCGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACGCGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTgc                                                                                             <  2:856532/139‑1 (MQ=255)

                                           agaCGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTgc                                                                                             <  1:321554/140‑1 (MQ=255)

                                              cGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCa                                                                                          >  1:163641/1‑140 (MQ=255)

                                                  ggCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTa                                                                                      >  2:751364/1‑140 (MQ=255)

                                                   gCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTgc                                                                                             >  2:972018/1‑132 (MQ=255)

                                                   gCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTgc                                                                                             <  1:972018/132‑1 (MQ=255)

                                                   gCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTaa                                                                                     >  2:572823/1‑140 (MQ=255)

                                                       aaaCATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCa                                                                                 >  2:413082/1‑140 (MQ=255)

                                                       aaaCATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCa                                                                                 <  1:173016/140‑1 (MQ=255)

                                                       aaaCATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCa                                                                                 >  1:512701/1‑140 (MQ=255)

                                                       aaaCATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCa                                                                                 >  1:184144/1‑140 (MQ=255)

                                                        aaCATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCa                                                                                 >  2:35337/1‑140 (MQ=255)

                                                         aCATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAAt                                                                               <  1:347393/140‑1 (MQ=255)

                                                            tatGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGAc                                                                            <  1:91937/140‑1 (MQ=255)

                                                             atGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCt                                                                                       <  1:74267/128‑1 (MQ=255)

                                                             atGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCt                                                                                       >  2:74267/1‑128 (MQ=255)

                                                               gtgtCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTT‑‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAAt                                                                               <  2:618291/133‑1 (MQ=255)

                                                               gtgtCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTT‑‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAAt                                                                               >  1:618291/1‑133 (MQ=255)

                                                                tgtCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAAt                                                                        >  1:652077/1‑140 (MQ=255)

                                                                  tcgtcgtcgTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGc                                                                      >  2:240414/1‑140 (MQ=255)

                                                                  tcgtcgtcgTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGc                                                                      >  2:382294/1‑140 (MQ=255)

                                                                   cgtcgtcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGcc                                                                     <  2:577982/140‑1 (MQ=255)

                                                                   cgtcgtcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGcc                                                                     >  2:481002/1‑140 (MQ=255)

                                                                   cgtcgtcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGcc                                                                     >  1:1121765/1‑140 (MQ=255)

                                                                    gtcgtcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCg                                                                    >  1:683001/1‑140 (MQ=255)

                                                                     tcgtcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCg                                                                    >  1:645618/1‑140 (MQ=255)

                                                                     tcgtcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGa                                                                   >  2:2868/1‑140 (MQ=255)

                                                                     tcgtcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGa                                                                   <  1:240414/140‑1 (MQ=255)

                                                                     tcgtcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGa                                                                   <  2:51800/140‑1 (MQ=255)

                                                                      cgtcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGa                                                                   <  1:35337/140‑1 (MQ=255)

                                                                      cgtcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGAt                                                                  >  2:561602/1‑140 (MQ=255)

                                                                       gtcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATg                                                                 >  2:228575/1‑140 (MQ=255)

                                                                        tcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATgg                                                                <  2:683001/140‑1 (MQ=255)

                                                                        tcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATgg                                                                <  1:731641/140‑1 (MQ=255)

                                                                        tcgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATgg                                                                <  1:572823/140‑1 (MQ=255)

                                                                         cgtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTCCCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATgg                                                                <  2:645618/140‑1 (MQ=255)

                                                                          gtGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGt                                                              >  2:798583/1‑140 (MQ=255)

                                                                               tAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACt                                                         <  2:399060/140‑1 (MQ=255)

                                                                                       gCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACtttt                                                 >  2:79601/1‑140 (MQ=255)

                                                                                       gCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACtttt                                                 >  1:238996/1‑140 (MQ=255)

                                                                                       gCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACtttt                                                 >  2:608144/1‑140 (MQ=255)

                                                                                        ccTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTc                                                <  2:581653/140‑1 (MQ=255)

                                                                                         cTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCa                                               <  2:535105/140‑1 (MQ=255)

                                                                                            ccGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGa                                            <  1:751364/140‑1 (MQ=255)

                                                                                            ccGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGa                                            <  1:2868/140‑1 (MQ=255)

                                                                                            ccGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGa                                            >  1:428940/1‑140 (MQ=255)

                                                                                             cGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAg                                           <  2:193614/140‑1 (MQ=255)

                                                                                                       gAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAc                                 <  1:481002/140‑1 (MQ=255)

                                                                                                       gAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAc                                 >  1:160931/1‑140 (MQ=255)

                                                                                                       gAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAc                                 <  1:464752/140‑1 (MQ=255)

                                                                                                          cAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctc                              <  1:29885/140‑1 (MQ=255)

                                                                                                           aGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctct                             <  2:163641/140‑1 (MQ=255)

                                                                                                            gTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctctc                            >  2:464079/1‑140 (MQ=255)

                                                                                                            gTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctctc                            >  2:1076454/1‑140 (MQ=255)

                                                                                                            gTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctctc                            >  2:565121/1‑140 (MQ=255)

                                                                                                            gTTCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctctc                            >  1:649256/1‑140 (MQ=255)

                                                                                                             ttCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAATAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctctct                           >  2:443793/1‑140 (MQ=255)

                                                                                                             ttCTGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctctct                           >  2:1095696/1‑140 (MQ=255)

                                                                                                                tGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATg                                   <  1:181037/129‑1 (MQ=255)

                                                                                                                tGCCAGCCAAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATg                                   >  2:181037/1‑129 (MQ=255)

                                                                                                                      ccaAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAc                                 >  1:908148/1‑125 (MQ=255)

                                                                                                                      ccaAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAc                                 <  2:908148/125‑1 (MQ=255)

                                                                                                                      ccaAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAt                  >  2:287865/1‑140 (MQ=255)

                                                                                                                       caAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctctct                           <  1:815212/130‑1 (MQ=255)

                                                                                                                       caAAAAAGCAAGCTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctctct                           >  2:815212/1‑130 (MQ=255)

                                                                                                                            aagcaagcTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATccc            <  1:413082/140‑1 (MQ=255)

                                                                                                                            aagcaagaTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATccc            <  2:562072/140‑1 (MQ=255)

                                                                                                                                aagcTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATc              <  1:264377/134‑1 (MQ=255)

                                                                                                                                aagcTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATc              >  2:264377/1‑134 (MQ=255)

                                                                                                                                aagcTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCCCAAc        >  2:155330/1‑140 (MQ=255)

                                                                                                                                 agcTGGTTTTC‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCCCAACt       <  1:104371/140‑1 (MQ=255)

                                                                                                                                     gttttc‑‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCCCAACTTAATc  <  1:1095696/138‑1 (MQ=255)

                                                                                                                                     gttttc‑‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCCCAACTTAATc  <  1:464079/138‑1 (MQ=255)

                                                                                                                                     gttttc‑‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCCCAACTTAATc  <  1:1024390/138‑1 (MQ=255)

                                                                                                                                     gttttc‑‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCCCAACTTAATc  <  1:1076454/138‑1 (MQ=255)

                                                                                                                                     gttttc‑‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCCCAACTTAATc  <  2:428940/138‑1 (MQ=255)

GAGGAAGCATGGCGGGATCACGACAGAAAACCACGGGCCAGTGAGACGTTGGCATAAACATATGTGTCGTCGTCGTGATTAACACACGCCTGCCGCTAGCCTGGAGCAGTTCTGCCAGCCAAAAAAGCAAGCTGGTTTT‑‑CCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCCCAACTTAATC  >  NC_000913/3014829‑3015101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A