Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,155,841	T→C	F163L (TTC→CTC)	yqhD →	NADPH‑dependent aldehyde reductase YqhD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,155,841	0	T	C	100.0%	187.9 / NA	50	F163L (TTC→CTC)	yqhD	NADPH‑dependent aldehyde reductase YqhD
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/25); total (25/25)

CATTCTGCAAACGGGCGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTA  >  NC_000913/3155708‑3155962

caTTCTGCAAACGGGCGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCAtt                                                                                                                     <  2:368685/140‑1 (MQ=255)

caTTCTGCAAACGGGCGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGAGCTCCAtt                                                                                                                     >  2:884895/1‑140 (MQ=255)

    cTGCAAACGGGCGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGc                                                                                                                 <  1:159823/140‑1 (MQ=255)

     tGCAAACGGGTGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGcc                                                                                                                >  2:168106/1‑140 (MQ=255)

          aCGGGCGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGt                                                                                                           >  2:546808/1‑140 (MQ=255)

            gggCGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTc                                                                                                         >  2:605724/1‑140 (MQ=255)

               cGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGc                                                                                                      <  2:1127064/140‑1 (MQ=255)

               cGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGCAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGc                                                                                                      >  2:646950/1‑140 (MQ=255)

                        gaTTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTg                                                                                             >  2:138056/1‑140 (MQ=255)

                               aGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCt                                                                                      <  1:138056/140‑1 (MQ=255)

                                  gcCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGa                                                                                   >  2:910822/1‑140 (MQ=255)

                                   ccATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGAt                                                                                  >  2:914787/1‑140 (MQ=255)

                                    cATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATc                                                                                 <  1:513281/140‑1 (MQ=255)

                                    cATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATc                                                                                 <  1:919358/140‑1 (MQ=255)

                                    cATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATc                                                                                 <  1:168106/140‑1 (MQ=255)

                                         cGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGAGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGtt                                                                            <  1:884895/140‑1 (MQ=255)

                                                 tgtgtgCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTa                                                                    >  2:735860/1‑140 (MQ=255)

                                                  gtgtgCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTac                                                                   >  2:84411/1‑140 (MQ=255)

                                                           cGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTgccg                                                          <  1:84411/140‑1 (MQ=255)

                                                             cTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTgccgcc                                                        >  2:123809/1‑140 (MQ=255)

                                                               gCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCcgccgc                                                      >  2:91310/1‑140 (MQ=255)

                                                               gCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCcgccgc                                                      <  1:735860/140‑1 (MQ=255)

                                                                  aGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTc                                                   <  1:123809/140‑1 (MQ=255)

                                                                      aCCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGt                                               >  2:215299/1‑140 (MQ=255)

                                                                          gTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCt                                           >  2:584318/1‑140 (MQ=255)

                                                                            tCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTaa                                         <  1:296956/140‑1 (MQ=255)

                                                                                     aaCGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGt                                >  1:921516/1‑140 (MQ=255)

                                                                                     aaCGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGt                                >  1:59659/1‑140 (MQ=255)

                                                                                     aaCGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGt                                >  2:334054/1‑140 (MQ=255)

                                                                                     aaCGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGt                                >  1:205781/1‑140 (MQ=255)

                                                                                     aaCGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGATGGCTAACGGCGTAGt                                >  2:1019191/1‑140 (MQ=255)

                                                                                       cGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTgg                              >  1:623064/1‑140 (MQ=255)

                                                                                          aGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACg                           <  2:623064/140‑1 (MQ=255)

                                                                                          aGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACg                           <  1:646950/140‑1 (MQ=255)

                                                                                          aGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACg                           <  2:205781/140‑1 (MQ=255)

                                                                                          aGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCATGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACg                           <  1:675209/140‑1 (MQ=255)

                                                                                            gcgcGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGcc                         >  2:1113291/1‑140 (MQ=255)

                                                                                            gcgcGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGcc                         <  1:910822/140‑1 (MQ=255)

                                                                                            gcgcGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGcc                         <  1:893057/140‑1 (MQ=255)

                                                                                                  tGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTa                   <  2:79380/140‑1 (MQ=255)

                                                                                                    aTCTCCCGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTaca                 <  1:584318/140‑1 (MQ=255)

                                                                                                         ccGTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCg            <  1:605724/140‑1 (MQ=255)

                                                                                                           gTAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTg          >  1:1095496/1‑140 (MQ=255)

                                                                                                            tAAAACCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTgg         >  2:624928/1‑140 (MQ=255)

                                                                                                              aaaCCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGaa       <  2:1095496/140‑1 (MQ=255)

                                                                                                               aaCCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAAc      <  2:930429/140‑1 (MQ=255)

                                                                                                                aCCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACa     >  1:375805/1‑140 (MQ=255)

                                                                                                                aCCACAGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACa     <  1:215299/140‑1 (MQ=255)

                                                                                                                   acaGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTa  <  2:375805/140‑1 (MQ=255)

                                                                                                                   acaGGCGACAAGCAGGCGCTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTa  >  2:976114/1‑140 (MQ=255)

CATTCTGCAAACGGGCGGTAAAGAGATTAAAAGCGCCATCCCGATGGGCTGTGTGCTGACGCTGCCAGCAACCGGTTCAGAATCCAACGCAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTA  >  NC_000913/3155708‑3155962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A