Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	51.4	1736588	99.0%	1719222	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,855,947	T→C	T9A (ACG→GCG)	ydjG ←	NADH‑dependent methylglyoxal reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,855,947	0	T	C	100.0%	128.5 / NA	37	T9A (ACG→GCG)	ydjG	NADH‑dependent methylglyoxal reductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/19); total (18/19)

GATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCGACGCTTAGAACCGAGATAGCCGCCGTTGCA  >  NC_000913/1855818‑1856077

gATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTaaa                                                                                                                          <  2:348956/140‑1 (MQ=255)

 aTTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAg                                                                                                                         >  2:680638/1‑140 (MQ=255)

 aTTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAg                                                                                                                         >  1:618158/1‑140 (MQ=255)

   tAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGt                                                                                                                       <  2:304360/140‑1 (MQ=255)

           caACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCttttt                                                                                                               >  2:694463/1‑140 (MQ=255)

               gATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTaa                                                                                                                           <  2:174738/124‑1 (MQ=255)

               gATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTaa                                                                                                                           >  1:174738/1‑124 (MQ=255)

                 tGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTg                                                                                                         >  1:822959/1‑140 (MQ=255)

                    gCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCt                                                                                                      <  1:257543/140‑1 (MQ=255)

                           gAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTAt                                                                                               <  1:642000/140‑1 (MQ=255)

                              tCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCt                                                                                            <  2:822959/140‑1 (MQ=255)

                                            atTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAg                                                                              >  1:683209/1‑140 (MQ=255)

                                              ttGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCa                                                                            <  2:618158/140‑1 (MQ=255)

                                                 ccGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAAtt                                                                         >  2:680700/1‑140 (MQ=255)

                                                  cGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTAttct                                                                                   <  1:135851/129‑1 (MQ=255)

                                                  cGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTAttct                                                                                   >  2:135851/1‑129 (MQ=255)

                                                   gATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTcc                                                                                <  2:431776/131‑1 (MQ=255)

                                                   gATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTcc                                                                                >  1:431776/1‑131 (MQ=255)

                                                      cGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCg                                                                    >  2:321860/1‑140 (MQ=255)

                                                         gaTCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGAcc                                                                 <  2:556114/140‑1 (MQ=255)

                                                          aTCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCa                                                                <  2:814343/140‑1 (MQ=255)

                                                                      tGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGtttt                                                    >  2:138551/1‑140 (MQ=255)

                                                                           gACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACg                                               <  2:833494/140‑1 (MQ=255)

                                                                           gACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACg                                               >  1:833494/1‑140 (MQ=255)

                                                                           gACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACg                                               >  2:725123/1‑140 (MQ=255)

                                                                           gACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACg                                               >  1:110339/1‑140 (MQ=255)

                                                                            aCCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGc                                              <  2:683209/140‑1 (MQ=255)

                                                                             ccgccgCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGcc                                             >  2:287745/1‑140 (MQ=255)

                                                                                          cccATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCg                                <  1:694463/140‑1 (MQ=255)

                                                                                          cccATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCg                                <  1:360275/140‑1 (MQ=255)

                                                                                              tGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCGACGc                            >  2:117695/1‑140 (MQ=255)

                                                                                               gTCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCGACGCt                           <  2:156116/140‑1 (MQ=255)

                                                                                                tCCCCAACCCCATTCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCGACGCtt                          <  1:205701/140‑1 (MQ=255)

                                                                                                            ttCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCGACGCTTAGAACCGAGATa              >  2:87281/1‑140 (MQ=255)

                                                                                                            ttCGCGAAAGCGTAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCGACGCTTAGAACCGAGATa              >  2:42374/1‑140 (MQ=255)

                                                                                                                        tAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCGACGCTTAGAACCGAGATAGCCGCCGTTGCa  <  1:87281/140‑1 (MQ=255)

                                                                                                                        tAATATCCGCTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCGACGCTTAGAACCGAGATAGCCGCCGTTGCa  <  1:117695/140‑1 (MQ=255)

GATTAATGCCACAACGATGGGCTTCAAGAATCGTATCAATACATATTTGCCGATCGAGATCGCCATTCCATGCAGGACCGCCGCCAATGGCCCATGTCCCCAACCCCATTCGCGAAAGCGTAATATCCGTTGTGCCTAAAGGTATCTTTTTCATTTGCCTTCATTATCCTTCGTATTCTTCCAGCAATTGTTCGACCAGCTTTCTGTTTTTTACGCCGGTGGTGGCACCGACGCTTAGAACCGAGATAGCCGCCGTTGCA  >  NC_000913/1855818‑1856077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A