Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	51.4	1736588	99.0%	1719222	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,199,409	T→C	intergenic (+2111/‑870)	yehF → / → yehI	putative protein YehF/DUF4132 domain‑containing protein YehI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,199,409	0	T	C	95.5%	141.6 / ‑5.8	44	intergenic (+2111/‑870)	yehF/yehI	putative protein YehF/DUF4132 domain‑containing protein YehI
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (24/18); minor base A (1/1); total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGAGAGCCGCGAACTCACGGTGGCGGAGCTTGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCTTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGC  >  NC_000913/2199280‑2199545

ccGAGAGCCGCGAGCTCACGGTGGCGGAGCTTGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGtt                                                                                                                                <  1:512426/140‑1 (MQ=255)

     agCCGCGAACTCACGGTGGCGGAGCTTGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGc                                                                                                                           >  1:846167/1‑140 (MQ=255)

          cgAACTCACGGTGGCGGAGCTTGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGAt                                                                                                                      >  1:720625/1‑140 (MQ=255)

                      ggCGGAGCTTGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAgc                                                                                                          <  1:332204/140‑1 (MQ=255)

                        cGGAGCTTGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAgccg                                                                                                        <  2:449478/140‑1 (MQ=255)

                        cGGAGCTTGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAgccg                                                                                                        <  2:304964/140‑1 (MQ=255)

                                 aaGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGa                                                                                               >  1:288382/1‑140 (MQ=255)

                                       gggTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAgc                                                                                                          <  1:256731/123‑1 (MQ=255)

                                       gggTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAgc                                                                                                          >  2:256731/1‑123 (MQ=255)

                                                   gATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCAGCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCaaa                                                                                     <  2:97728/132‑1 (MQ=255)

                                                   gATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAACACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCaaa                                                                                     >  1:97728/1‑132 (MQ=255)

                                                   gATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACaa                                                                             >  2:79834/1‑140 (MQ=255)

                                                   gATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACaa                                                                             >  2:301763/1‑140 (MQ=255)

                                                   gATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACaa                                                                             >  2:280681/1‑140 (MQ=255)

                                                   gATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACaa                                                                             >  2:684038/1‑140 (MQ=255)

                                                    acctcGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACaaa                                                                            <  1:683848/138‑1 (MQ=255)

                                                        cGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCg                                                                        <  2:720625/140‑1 (MQ=255)

                                                               gatgatAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAg                                                                          >  1:766048/1‑131 (MQ=255)

                                                               gatgatAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGAtgatga                                                                 >  1:327129/1‑140 (MQ=255)

                                                               gatgatAACGGTTCGCTGCTGCTCGATTTTGCCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCTGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAg                                                                          <  2:766048/131‑1 (MQ=255)

                                                                         gTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCg                                                       >  2:814673/1‑140 (MQ=255)

                                                                         gTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCATAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCg                                                       >  1:50257/1‑140 (MQ=255)

                                                                            cGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCg                                                       <  1:339474/137‑1 (MQ=255)

                                                                            cGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCg                                                       >  2:339474/1‑137 (MQ=255)

                                                                            cGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAAc                                                    >  1:123593/1‑140 (MQ=255)

                                                                              ctgctgctgGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGa                                                  >  2:66522/1‑140 (MQ=255)

                                                                               tgctgctgGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGAt                                                 >  1:631294/1‑140 (MQ=255)

                                                                               tgctgctgGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGAt                                                 >  2:270376/1‑140 (MQ=255)

                                                                               tgctgctgGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGAt                                                 >  2:307645/1‑140 (MQ=255)

                                                                                    ctgGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACg                                                   >  1:456895/1‑133 (MQ=255)

                                                                                    ctgGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACg                                                   <  2:456895/133‑1 (MQ=255)

                                                                                               cccACGGCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACa                                 <  2:865274/140‑1 (MQ=255)

                                                                                                    ggCAGTTCACCGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTg                            <  2:327129/140‑1 (MQ=255)

                                                                                                    ggCAGTTCACCGTCAGCTTTGATGAAACCATAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTg                            <  2:50257/140‑1 (MQ=255)

                                                                                                              cGTCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAg                  >  2:813640/1‑140 (MQ=255)

                                                                                                              cGTCAGCTTTGATGAAACCCTAAAACCGTATGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAg                  <  2:302786/140‑1 (MQ=255)

                                                                                                              cGTCAGCTTTGATGAAACCCTAAAACCGTATGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAg                  >  1:302786/1‑140 (MQ=255)

                                                                                                                tCAGCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGAt                <  1:66522/140‑1 (MQ=255)

                                                                                                                   gCTTTGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATgcg             >  1:86537/1‑140 (MQ=255)

                                                                                                                     tttGATGAAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGcgcg           >  1:10506/1‑140 (MQ=255)

                                                                                                                           gaAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCAt     <  1:301763/140‑1 (MQ=255)

                                                                                                                           gaAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCAt     <  2:846167/140‑1 (MQ=255)

                                                                                                                           gaAACCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCAt     <  1:586284/140‑1 (MQ=255)

                                                                                                                              aCCCTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATcgc  >  1:638990/1‑140 (MQ=255)

CCGAGAGCCGCGAACTCACGGTGGCGGAGCTTGAAGATCGGGTTAGCACCGGATCTCGGTCTGGATGATAACGGTTCGCTGCTGCTGGATTTTGGCCCACGGCAGTTCACCGTCAGCTTTGATGAAACCTTAAAACCGTTTGTGCGTGATGTTTCCGGCAGCCGCCTGAAAGACCTGCCCAAACCGAACAAAAGCGATGATGAAACGCGGGCGAACGATGCGGTTAACCGCTACAAATTGCTGAAAAAAGATGCGCGTACCATCGC  >  NC_000913/2199280‑2199545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A