Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	51.4	1736588	99.0%	1719222	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,251,892	A→G	T65A (ACA→GCA)	yeiI →	putative sugar kinase YeiI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,251,892	0	A	G	100.0%	110.0 / NA	32	T65A (ACA→GCA)	yeiI	putative sugar kinase YeiI
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/19); total (13/19)

GCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGT  >  NC_000913/2251756‑2252028

gcagAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGcaa                                                                                                                                       <  2:653028/140‑1 (MQ=255)

 cagAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGcaat                                                                                                                                      <  1:77462/140‑1 (MQ=255)

 cagAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGcaat                                                                                                                                      <  2:60204/140‑1 (MQ=255)

              cGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATAtt                                                                                                                         >  1:1365/1‑140 (MQ=255)

                gACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTcg                                                                                                                       <  2:160879/140‑1 (MQ=255)

                gACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTcg                                                                                                                       <  2:35966/140‑1 (MQ=255)

                 aCATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTcgc                                                                                                                      <  2:572521/140‑1 (MQ=255)

                            aTCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGa                                                                                                           <  1:571425/140‑1 (MQ=255)

                                gCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATc                                                                                                       >  2:333821/1‑140 (MQ=255)

                                 ccGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATcc                                                                                                      >  2:465140/1‑140 (MQ=255)

                                                     atatTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTtct                                                                                  <  1:178763/140‑1 (MQ=255)

                                                          atGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATcc                                                                             <  1:333821/140‑1 (MQ=255)

                                                          atGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATcc                                                                             <  1:303400/140‑1 (MQ=255)

                                                                ttAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTtct                                                                                  >  1:804286/1‑129 (MQ=255)

                                                                ttAATGCGCAAAGGCCGGATTAAAGGCAAAGGCTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTtct                                                                                  <  2:804286/129‑1 (MQ=255)

                                                                 tAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACa                                                                      >  1:634901/1‑140 (MQ=255)

                                                                 tAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACa                                                                      <  1:665090/140‑1 (MQ=255)

                                                                      cgcAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCa                                                                 <  2:853388/140‑1 (MQ=255)

                                                                                     aaaGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATAAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAgcgggcgg                                                  >  1:58653/1‑140 (MQ=255)

                                                                                       aGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGgcggcg                                                <  2:419648/140‑1 (MQ=255)

                                                                                         gCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTg                                              <  2:634901/140‑1 (MQ=255)

                                                                                              ggTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACg                                         <  2:413317/140‑1 (MQ=255)

                                                                                                   cATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACa                                    >  2:816761/1‑140 (MQ=255)

                                                                                                    aTTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACAt                                   <  1:816761/140‑1 (MQ=255)

                                                                                                     ttCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATc                                  <  2:330998/140‑1 (MQ=255)

                                                                                                     ttCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATc                                  <  2:529719/140‑1 (MQ=255)

                                                                                                         cACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGccc                              >  1:548991/1‑140 (MQ=255)

                                                                                                         cACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGccc                              >  1:383223/1‑140 (MQ=255)

                                                                                                         cACCGAGCAGGAATACTGCGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACAGCGccc                              >  2:766648/1‑140 (MQ=255)

                                                                                                                           cGTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAg            >  2:424195/1‑140 (MQ=255)

                                                                                                                            gTAGTGGTGGGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAgg           >  1:288542/1‑140 (MQ=255)

                                                                                                                                   tggGGGCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGAc    >  1:197090/1‑140 (MQ=255)

                                                                                                                                     ggggCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTg      <  2:817666/136‑1 (MQ=255)

                                                                                                                                     ggggCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGt  >  2:563831/1‑140 (MQ=255)

                                                                                                                                     ggggCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGt  >  2:150488/1‑140 (MQ=255)

GCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAATGCGCAAAGGCCGGATTAAAGGCAAAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGT  >  NC_000913/2251756‑2252028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A