Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	51.4	1736588	99.0%	1719222	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,391,857	C→T	T395T (ACG→ACA)	nuoM ←	NADH:quinone oxidoreductase subunit M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,391,857	0	C	T	100.0%	118.4 / NA	35	T395T (ACG→ACA)	nuoM	NADH:quinone oxidoreductase subunit M
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/21); total (14/21)

CGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGC  >  NC_000913/2391728‑2391985

cGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCg                                                                                                                        <  1:814389/140‑1 (MQ=255)

  ccAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCa                                                                                                                      >  2:582345/1‑140 (MQ=255)

   cAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCaa                                                                                                                     <  1:231491/140‑1 (MQ=255)

   cAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCaa                                                                                                                     <  1:802477/140‑1 (MQ=255)

       gAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGaa                                                                                                                 <  1:582345/140‑1 (MQ=255)

             aCAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGa                                                                                                           <  1:584620/140‑1 (MQ=255)

                        gACCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCa                                                                                                <  2:139496/140‑1 (MQ=255)

                         aCCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAg                                                                                               <  1:12286/140‑1 (MQ=255)

                         aCCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTATTGCCACCGCAAAGaa                                                                                                                 >  2:81383/1‑122 (MQ=255)

                         aCCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTATTGCCACCGCAAAGaa                                                                                                                 <  1:81383/122‑1 (MQ=255)

                           cAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGcc                                                                                             >  1:427560/1‑140 (MQ=255)

                               ccAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGG‑AGCTGCCGAAAAGAATCATAAATTCGCCGCCGAAGTTACCGGGGCCAGGCATCCCAAGGGTTGCCACCCCAAAGAACAGCGAAAGGGCTGCCGGCCATTTc                                                                                        >  2:483112/1‑140 (MQ=255)

                                 aaaGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCa                                                                                       <  2:785732/140‑1 (MQ=255)

                                 aaaGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCa                                                                                       <  2:829372/140‑1 (MQ=255)

                                   aGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATc                                                                                     >  2:664828/1‑140 (MQ=255)

                                        gagaTAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCt                                                                                >  2:82485/1‑140 (MQ=255)

                                        gagaTAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCt                                                                                >  2:538725/1‑140 (MQ=255)

                                        gagaTAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCt                                                                                >  2:528282/1‑140 (MQ=255)

                                              aCGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAg                                                                          <  1:323470/140‑1 (MQ=255)

                                               cGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGa                                                                         >  1:852107/1‑140 (MQ=255)

                                               cGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGa                                                                         >  2:392930/1‑140 (MQ=255)

                                                   aaTCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGc                                                                     <  2:499451/140‑1 (MQ=255)

                                                    aTCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGcc                                                                    >  2:341349/1‑140 (MQ=255)

                                                       aCCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGc                                                                     <  1:846590/136‑1 (MQ=255)

                                                       aCCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGc                                                                     >  2:846590/1‑136 (MQ=255)

                                                               aaCCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCa                                                         <  1:582672/140‑1 (MQ=255)

                                                                     gAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTcgc                                                   <  1:528282/140‑1 (MQ=255)

                                                                      aaGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTcgcg                                                  >  2:379074/1‑140 (MQ=255)

                                                                              gAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGa                                          <  1:538725/140‑1 (MQ=255)

                                                                                    aaTCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTa                                              <  2:312598/130‑1 (MQ=255)

                                                                                    aaTCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTa                                              >  1:312598/1‑130 (MQ=255)

                                                                                      tCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCa                                  <  1:664828/140‑1 (MQ=255)

                                                                                      tCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCa                                  >  1:334303/1‑140 (MQ=255)

                                                                                             ttCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCt                           <  1:379074/140‑1 (MQ=255)

                                                                                                   gacgaAGTTACCGGTGCCAGGCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACcac                     <  1:509857/140‑1 (MQ=255)

                                                                                                                      ggCATCCCAAGTGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACccgc  <  2:354113/140‑1 (MQ=255)

CGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCACCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACAGTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGC  >  NC_000913/2391728‑2391985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A