Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F28 I1 R1
|
176 |
31.5 |
1082462 |
98.8% |
1069472 |
138.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,394,574 |
T→C |
Y158C (TAC→TGC) |
nuoL ← |
NADH:quinone oxidoreductase subunit L |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,394,574 | 0 | T | C | 100.0%
| 84.5
/ NA
| 24 | Y158C (TAC→TGC) | nuoL | NADH:quinone oxidoreductase subunit L |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/10); total (14/10) |
CACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGC > NC_000913/2394444‑2394703
|
caccaTTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAAcc > 2:224255/1‑140 (MQ=255)
aTTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGAt < 2:442954/140‑1 (MQ=255)
ttCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATca > 1:173615/1‑140 (MQ=255)
gAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGAt < 1:224255/140‑1 (MQ=255)
gggTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGcac < 2:284656/140‑1 (MQ=255)
tGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAgg > 2:76121/1‑140 (MQ=255)
tGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTcc > 1:273966/1‑140 (MQ=255)
tGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAgg < 2:412505/140‑1 (MQ=255)
cGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAg > 1:37734/1‑125 (MQ=255)
cGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAg < 2:37734/125‑1 (MQ=255)
aGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGtt > 1:433414/1‑140 (MQ=255)
aGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGtt > 2:175567/1‑140 (MQ=255)
aGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGtt > 2:537537/1‑140 (MQ=255)
aGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGtt > 2:402730/1‑140 (MQ=255)
cacGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGa > 1:194246/1‑140 (MQ=255)
ggTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAacca < 1:537537/140‑1 (MQ=255)
ggTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAacca > 1:66308/1‑140 (MQ=255)
acgacgAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCAt > 1:483899/1‑140 (MQ=255)
aCGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCg > 1:101197/1‑140 (MQ=255)
aCGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCg < 2:29842/140‑1 (MQ=255)
ttGCCGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAgg < 1:76121/140‑1 (MQ=255)
ccGCTGCGCCATTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTg > 2:234003/1‑140 (MQ=255)
aTTCTTCGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCga < 2:66308/140‑1 (MQ=255)
cGGATCGGTGCAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGc < 1:234003/140‑1 (MQ=255)
|
CACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGC > NC_000913/2394444‑2394703
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
