Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	51.4	1736588	99.0%	1719222	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,826,251	C→T	G140G (GGC→GGT)	srlA →	sorbitol‑specific PTS enzyme IIC2 component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,826,251	0	C	T	100.0%	97.4 / NA	29	G140G (GGC→GGT)	srlA	sorbitol‑specific PTS enzyme IIC2 component
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/13); total (16/13)

ACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGG  >  NC_000913/2826119‑2826382

aCCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTTCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTgg                                                                                                                              >  2:803721/1‑140 (MQ=255)

aCCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTgg                                                                                                                              >  2:411374/1‑140 (MQ=255)

  ccAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCg                                                                                                                            <  1:691118/140‑1 (MQ=255)

   cAGCTACTACGCGGGGGCCTTTTATACTTGCCACTCAATAAATGGCCTCTTCCCCCAAATCACCCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGGGGTCTGACAACGCAGAACCTGCCACTTGGTCCACTGGCgg                                                                                                                           <  2:866416/140‑1 (MQ=255)

     gCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGt                                                                                                                          <  2:450691/139‑1 (MQ=255)

     gCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGt                                                                                                                          >  1:450691/1‑139 (MQ=255)

              cggcggCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATctg                                                                                                                >  1:275788/1‑140 (MQ=255)

                gcggcCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATctgct                                                                                                              <  2:275788/140‑1 (MQ=255)

                       ctTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCtggttggt                                                                                                       >  1:365713/1‑140 (MQ=255)

                        ttATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTtggtc                                                                                                      >  2:73853/1‑140 (MQ=255)

                          ataGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTtggtctg                                                                                                    <  1:411374/140‑1 (MQ=255)

                                gCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCtgg                                                                                                            <  2:727899/126‑1 (MQ=255)

                                gCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCtgg                                                                                                            >  1:727899/1‑126 (MQ=255)

                                gCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCa                                                                                                <  1:631657/138‑1 (MQ=255)

                                gCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCa                                                                                                >  2:631657/1‑138 (MQ=255)

                                 ccACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGGGAGGTATCTGCTGGTTGGGCTGGTCCCCa                                                                                             >  2:169332/1‑140 (MQ=255)

                                  cACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGGGGTTTCTGCTGGTTGGTCTGGTCACCaa                                                                                            >  2:429778/1‑140 (MQ=255)

                                         tgaatgGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGCTATCTTCTTGTTGGTCTCGTTCCCCATttcttc                                                                                     >  2:546339/1‑140 (MQ=255)

                                            atgGCCTCTTCCCCCATATCACCCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCcgc                                                                                  <  1:295699/140‑1 (MQ=255)

                                               gCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGc                                                                               <  2:820748/140‑1 (MQ=255)

                                                   ctTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGGTATCTTCTGGGTGGTCTGGTCACCAATTTCTTTCGCGGCTggg                                                                           >  1:561887/1‑140 (MQ=255)

                                                     cagcccATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTg                                                                         <  1:676829/137‑1 (MQ=255)

                                                      cccccATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGa                                                                        <  1:73853/140‑1 (MQ=255)

                                                      cccccATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGa                                                                        <  1:429778/140‑1 (MQ=255)

                                                                   ccTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGGGTTATTTGCTGGTTGGTCTGGTCACCAATTTTTTCCGCGGCTGGGTGACCGATCTGacctg                                                           >  2:676829/1‑138 (MQ=255)

                                                                       gCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGcc                                                       >  1:793431/1‑140 (MQ=255)

                                                                                        tttGGCATTGCCAGCGGTCTGACACCGCTGACCCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGAt                                      <  1:803721/139‑1 (MQ=255)

                                                                                                            gACAACGCTGAACCTGCCACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGGGACCGATCTGACCACCGCCATTTTTTAGAAAAAGATGGGCATTCAACTTGaaaaaa                  >  1:402215/1‑140 (MQ=255)

                                                                                                                            ccACTTGGTCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGGGACCGATCTGACCACCGCCATTTTTTAGAAAAAAATGGGCATTCAACTTGAACAAAAAATTCACCTGGCAgg  >  2:400994/1‑140 (MQ=255)

ACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGG  >  NC_000913/2826119‑2826382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A