Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	51.4	1736588	99.0%	1719222	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,181,329	G→A	P303P (CCG→CCA)	ygiC →	putative enzyme with ATPase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,181,329	0	G	A	100.0%	174.8 / NA	50	P303P (CCG→CCA)	ygiC	putative enzyme with ATPase activity
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/26); total (24/26)

CATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCT  >  NC_000913/3181206‑3181462

cATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAg                                                                                                                       >  1:313531/1‑140 (MQ=255)

 aTTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAgg                                                                                                                      <  2:123153/140‑1 (MQ=255)

    aTCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGGAGgcgc                                                                                                                   >  1:464093/1‑140 (MQ=255)

      ctcCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCaa                                                                                                                 <  1:666645/140‑1 (MQ=255)

             aaGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTc                                                                                                          <  2:658264/140‑1 (MQ=255)

               ggCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAGTATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGa                                                                                                        >  2:743378/1‑140 (MQ=255)

               ggCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGa                                                                                                        >  2:142160/1‑140 (MQ=255)

               ggCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGa                                                                                                        >  2:28220/1‑140 (MQ=255)

               ggCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGa                                                                                                        >  2:310367/1‑140 (MQ=255)

                      cTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTga                                                                                                 >  1:303840/1‑140 (MQ=255)

                            cTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACgg                                                                                           >  1:669437/1‑140 (MQ=255)

                              aCTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCa                                                                                         <  2:864353/140‑1 (MQ=255)

                                       gaTGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTg                                                                                <  2:303840/140‑1 (MQ=255)

                                          gTTCCCGAATCACCCGAACCTGCTTCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAag                                                                             >  2:6173/1‑140 (MQ=255)

                                          gTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAag                                                                             >  2:361785/1‑140 (MQ=255)

                                           ttCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAagc                                                                            <  2:313531/140‑1 (MQ=255)

                                           ttCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAagc                                                                            >  1:693879/1‑140 (MQ=255)

                                                gAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCgg                                                                       <  1:401420/140‑1 (MQ=255)

                                                gAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCgg                                                                       <  2:782625/140‑1 (MQ=255)

                                                     aCCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGt                                                                  <  1:310367/140‑1 (MQ=255)

                                                        cGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCg                                                               <  2:62865/140‑1 (MQ=255)

                                                        cGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCg                                                               <  2:761572/140‑1 (MQ=255)

                                                        cGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCg                                                               <  2:777642/140‑1 (MQ=255)

                                                        cGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCg                                                               >  2:828956/1‑140 (MQ=255)

                                                         gAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGt                                                              <  1:301497/140‑1 (MQ=255)

                                                         gAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGt                                                              <  1:444291/140‑1 (MQ=255)

                                                           aCCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTAt                                                            <  1:28220/140‑1 (MQ=255)

                                                              tgctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAGTGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGaa                                                                     <  1:4188/128‑1 (MQ=255)

                                                              tgctgcCCGCTTATTTTGCGGAAGATGATCATCCGCAAGTGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGaa                                                                     >  2:4188/1‑128 (MQ=255)

                                                                   cccGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCgaaga                                                    <  2:149628/140‑1 (MQ=255)

                                                                     cGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGaagaag                                                  >  2:685711/1‑140 (MQ=255)

                                                                      gCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAgg                                                 <  1:142160/140‑1 (MQ=255)

                                                                       cTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAggg                                                >  1:809862/1‑140 (MQ=255)

                                                                          aTTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGgatg                                             >  1:196294/1‑140 (MQ=255)

                                                                             ttGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGAtt                                          >  2:615225/1‑140 (MQ=255)

                                                                                  gAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCa                                     <  1:615225/140‑1 (MQ=255)

                                                                                             tcCGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGATCGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCAcc                          >  2:602694/1‑140 (MQ=255)

                                                                                               cGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCa                                     <  2:352830/127‑1 (MQ=255)

                                                                                               cGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCa                                     >  1:352830/1‑127 (MQ=255)

                                                                                               cGCAAATGGAAAAATATGTGGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCg                        >  2:312939/1‑140 (MQ=255)

                                                                                                                gtgGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGa       <  2:669437/140‑1 (MQ=255)

                                                                                                                gtgGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGa       <  1:312939/140‑1 (MQ=255)

                                                                                                                gtgGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGa       <  1:6173/140‑1 (MQ=255)

                                                                                                                 tgGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGc         <  1:34066/137‑1 (MQ=255)

                                                                                                                 tgGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGc         >  2:34066/1‑137 (MQ=255)

                                                                                                                 tgGTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGAc      >  2:732425/1‑140 (MQ=255)

                                                                                                                  ggTTAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACa     >  2:433918/1‑140 (MQ=255)

                                                                                                                     tAAACCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCt  <  1:685711/140‑1 (MQ=255)

                                                                                                                       aaCCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGa       <  2:168950/133‑1 (MQ=255)

                                                                                                                       aaCCAATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGa       >  1:168950/1‑133 (MQ=255)

CATTATCTCCAACAAGGCACTTCTACCGCTACTGTGGGAGATGTTCCCGAATCACCCGAACCTGCTGCCCGCTTATTTTGCGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCT  >  NC_000913/3181206‑3181462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A