Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	176	31.5	1082462	98.8%	1069472	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,181,423	G→A	V335I (GTT→ATT)	ygiC →	putative enzyme with ATPase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,181,423	0	G	A	100.0%	156.1 / NA	45	V335I (GTT→ATT)	ygiC	putative enzyme with ATPase activity
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/21); total (24/21)

CGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGAT  >  NC_000913/3181286‑3181545

cGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCA‑CGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTc                                                                                                                         >  1:75953/1‑140 (MQ=255)

  gAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCa                                                                                                                        >  2:234390/1‑140 (MQ=255)

             tcCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCAcc                                                                                                             >  2:42605/1‑140 (MQ=255)

               cGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCg                                                                                                           >  1:183173/1‑140 (MQ=255)

               cGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCg                                                                                                           >  1:527747/1‑140 (MQ=255)

                gCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGt                                                                                                          >  2:115107/1‑140 (MQ=255)

                              atGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAAt                                                                                                 >  2:298682/1‑135 (MQ=255)

                              atGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAAt                                                                                                 <  1:298682/135‑1 (MQ=255)

                               tgtgGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCg                                                                                           <  1:129881/140‑1 (MQ=255)

                                            aTCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGa                                                                                          <  1:127688/128‑1 (MQ=255)

                                            aTCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGa                                                                                          >  2:127688/1‑128 (MQ=255)

                                            aTCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGAGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCt                                                                              >  1:430988/1‑140 (MQ=255)

                                              cttctCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGa                                                                            >  1:68369/1‑140 (MQ=255)

                                              cttctCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGa                                                                            >  1:428425/1‑140 (MQ=255)

                                               ttctCCCGTGAAGGCGCAGACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGAt                                                                           <  2:93305/140‑1 (MQ=255)

                                                 ctcCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTg                                                                         >  1:427344/1‑140 (MQ=255)

                                                  tcCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTgg                                                                        <  1:395062/140‑1 (MQ=255)

                                                      gTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAgc                                                                    <  1:293322/140‑1 (MQ=255)

                                                         aaGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAgctgg                                                                 >  2:358566/1‑140 (MQ=255)

                                                          aGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAgctggc                                                                <  1:42605/140‑1 (MQ=255)

                                                           ggCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAgctggct                                                               >  2:327855/1‑140 (MQ=255)

                                                           ggCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAgctggct                                                               >  1:48487/1‑140 (MQ=255)

                                                                                 agaACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTGCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGa                                         >  2:48027/1‑140 (MQ=255)

                                                                                 agaACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGa                                         >  2:85461/1‑140 (MQ=255)

                                                                                   aaCGGCAAAACCGTTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAAt                                       <  2:532428/140‑1 (MQ=255)

                                                                                   aaCGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAAt                                       <  2:68369/140‑1 (MQ=255)

                                                                                     cGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTg                                     >  2:211428/1‑140 (MQ=255)

                                                                                     cGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTg                                     >  2:384268/1‑140 (MQ=255)

                                                                                      ggCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTgg                                    <  2:48487/140‑1 (MQ=255)

                                                                                      ggCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTgg                                    >  2:446520/1‑140 (MQ=255)

                                                                                        cAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCa                                  <  1:327855/140‑1 (MQ=255)

                                                                                        cAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCa                                  <  1:211428/140‑1 (MQ=255)

                                                                                          aaaCCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCAtt                                <  1:358566/140‑1 (MQ=255)

                                                                                              cATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTg                            <  1:234390/140‑1 (MQ=255)

                                                                                                ttGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCa                                  <  1:106393/132‑1 (MQ=255)

                                                                                                ttGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCa                                  >  2:106393/1‑132 (MQ=255)

                                                                                                  gAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGa                        <  1:446520/140‑1 (MQ=255)

                                                                                                       agcGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTg                   >  2:57872/1‑140 (MQ=255)

                                                                                                       agcGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTg                   <  1:384268/140‑1 (MQ=255)

                                                                                                        gcGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGc                  >  1:185959/1‑140 (MQ=255)

                                                                                                         cGGAAGGTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCAACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCa                 >  1:411919/1‑140 (MQ=255)

                                                                                                              ggTCCGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCAACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGAt            <  2:411919/140‑1 (MQ=255)

                                                                                                                 ccGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCAc         <  2:428425/140‑1 (MQ=255)

                                                                                                                  cGTATGGCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCAcc        <  1:115107/140‑1 (MQ=255)

                                                                                                                        gCGAAGAAGGGATGATTATTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATTACCCAGGat  <  2:75953/140‑1 (MQ=255)

CGGAAGATGATCATCCGCAAATGGAAAAATATGTGGTTAAACCGATCTTCTCCCGTGAAGGCGCAAACGTGTCGATCATTGAGAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGAT  >  NC_000913/3181286‑3181545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A