Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	51.4	1736588	99.0%	1719222	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,501,803	A→T	D179V (GAT→GTT)	frlB →	fructoselysine 6‑phosphate deglycase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,501,803	0	A	T	100.0%	188.3 / NA	55	D179V (GAT→GTT)	frlB	fructoselysine 6‑phosphate deglycase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (29/26); total (29/26)

CGGCGGAATTTAGCATTGATTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGATCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCGGGTCCGCTGCGTCCGCTGGG  >  NC_000913/3501674‑3501935

cggcggAATTTAGCATTGATTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAg                                                                                                                            >  1:694119/1‑140 (MQ=255)

    ggAATTTAGCATTGATTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGc                                                                                                                        <  2:602516/140‑1 (MQ=255)

    ggAATTTAGCATTGATTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGc                                                                                                                        <  2:257881/140‑1 (MQ=255)

             cATTGATTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGa                                                                                                                     <  2:779633/134‑1 (MQ=255)

             cATTGATTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGa                                                                                                                     >  1:779633/1‑134 (MQ=255)

              aTTGATTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCt                                                                                                              <  2:583620/140‑1 (MQ=255)

                tGATTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCg                                                                                                            >  1:156964/1‑140 (MQ=255)

                  aTTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCgg                                                                                                           >  2:23937/1‑139 (MQ=255)

                  aTTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCgg                                                                                                           <  1:23937/139‑1 (MQ=255)

                   ttATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTc                                                                                                         <  2:35592/140‑1 (MQ=255)

                          gCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGt                                                                                                  >  1:365378/1‑140 (MQ=255)

                           ccGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTa                                                                                                 <  1:41975/140‑1 (MQ=255)

                              aCTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGc                                                                                              >  1:99656/1‑140 (MQ=255)

                                   aTCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTg                                                                                         <  2:365378/140‑1 (MQ=255)

                                            aTTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGaaaa                                                                                >  1:779908/1‑140 (MQ=255)

                                            aTTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGaaaa                                                                                >  1:252697/1‑140 (MQ=255)

                                                   tgctgcTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGgccgc                                                                         <  2:99656/140‑1 (MQ=255)

                                                   tgctgcTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGgccgc                                                                         <  1:570831/140‑1 (MQ=255)

                                                        ctctgctACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCt                                                                    >  1:258075/1‑140 (MQ=255)

                                                        ctctgctACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCt                                                                    >  2:23448/1‑140 (MQ=255)

                                                                cAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTg                                                                  <  2:253515/134‑1 (MQ=255)

                                                                cAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTg                                                                  >  1:253515/1‑134 (MQ=255)

                                                                  gCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTg                                                          >  2:261283/1‑140 (MQ=255)

                                                                     tggtgCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGgcc                                                       >  2:449840/1‑140 (MQ=255)

                                                                     tggtgCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGgcc                                                       >  2:584708/1‑140 (MQ=255)

                                                                       gtgCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGgccag                                                     >  2:250238/1‑140 (MQ=255)

                                                                          cTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGgccagcca                                                  <  2:779908/140‑1 (MQ=255)

                                                                           tGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGccagccag                                                 >  2:760307/1‑140 (MQ=255)

                                                                             gagaTGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTg                                               >  2:59476/1‑140 (MQ=255)

                                                                                 tgatCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAGGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCg                                           <  2:694119/140‑1 (MQ=255)

                                                                                  gatCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCga                                          <  2:156964/140‑1 (MQ=255)

                                                                                  gatCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCga                                          >  2:221497/1‑140 (MQ=255)

                                                                                  gatCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCga                                          >  1:272849/1‑140 (MQ=255)

                                                                                  gatCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCga                                          >  2:428262/1‑140 (MQ=255)

                                                                                  gatCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCg                                           <  2:642678/139‑1 (MQ=255)

                                                                                  gatCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCg                                           >  1:642678/1‑139 (MQ=255)

                                                                                       cccGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGAtt                                     <  1:760307/140‑1 (MQ=255)

                                                                                         cGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTta                                   >  2:780384/1‑140 (MQ=255)

                                                                                          gCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTtat                                  >  2:288957/1‑140 (MQ=255)

                                                                                             tCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATAcc                               <  2:275571/140‑1 (MQ=255)

                                                                                             tCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATAcc                               >  2:601902/1‑140 (MQ=255)

                                                                                             tCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATAcc                               >  2:668013/1‑140 (MQ=255)

                                                                                                cgcCGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGtt                            >  2:779741/1‑140 (MQ=255)

                                                                                                  ccGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTtgc                          <  2:542971/140‑1 (MQ=255)

                                                                                                  ccGAACGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTtgc                          <  2:825337/140‑1 (MQ=255)

                                                                                                      aCGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCg                      >  1:165037/1‑140 (MQ=255)

                                                                                                      aCGCGGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCg                      >  1:467607/1‑140 (MQ=255)

                                                                                                       cgcgGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCgg                     <  1:59476/140‑1 (MQ=255)

                                                                                                         cgGAAATCGGCAAGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCGGGt                   >  1:216721/1‑140 (MQ=255)

                                                                                                                     aGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCGGGTCCGCTGCGTCCg       <  1:288957/140‑1 (MQ=255)

                                                                                                                     aGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCGGGTCCGCTGCGTCCg       <  1:710715/140‑1 (MQ=255)

                                                                                                                     aGATCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCGGGTCCGCTGCGTCCg       <  2:252697/140‑1 (MQ=255)

                                                                                                                       aTCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCGGGTCCGCTGCGTCCGCt     <  1:23448/140‑1 (MQ=255)

                                                                                                                       aTCAAAAACGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCGGGTCCGCTGCGTCCGCt     <  2:272849/140‑1 (MQ=255)

                                                                                                                          aaaaaCGTTCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCGGGTCCGCTGCGTCCGCTggg  <  1:668013/140‑1 (MQ=255)

CGGCGGAATTTAGCATTGATTATCAGGCCGACTGTATCTGGGAAATTCACCTGCTGCTCTGCTACAGCGTGGTGCTGGAGATGATCACCCGCCTCGCGCCGAACGCGGAAATCGGCAAGATCAAAAACGATCTCAAGCAGTTGCCGAATGCGCTCGGTCATCTGGTACGCACCTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTATACCGTTGCTGCGGGTCCGCTGCGTCCGCTGGG  >  NC_000913/3501674‑3501935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A