Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	176	31.5	1082462	98.8%	1069472	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,502,200	G→A	V311V (GTG→GTA)	frlB →	fructoselysine 6‑phosphate deglycase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,502,200	0	G	A	100.0%	133.1 / NA	39	V311V (GTG→GTA)	frlB	fructoselysine 6‑phosphate deglycase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/21); total (18/21)

CAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGT  >  NC_000913/3502065‑3502333

cAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCaa                                                                                                                                   <  2:7651/140‑1 (MQ=255)

cAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCaa                                                                                                                                   <  1:299269/140‑1 (MQ=255)

     atgaAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAg                                                                                                                              <  1:412080/140‑1 (MQ=255)

         aaGTCGCCACACCACCGACCGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCTCCCGTGGCTGGCCCCGTTCCTGATGTTCGTACCAATGGAGTGGc                                                                                                                          <  2:376748/140‑1 (MQ=255)

          aGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGct                                                                                                                         <  1:460863/140‑1 (MQ=255)

          aGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGct                                                                                                                         <  1:6684/140‑1 (MQ=255)

          aGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGct                                                                                                                         <  1:279473/140‑1 (MQ=255)

             cGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTg                                                                                                                      >  1:416612/1‑140 (MQ=255)

             cGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTg                                                                                                                      >  1:165585/1‑140 (MQ=255)

             cGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTg                                                                                                                      >  2:409194/1‑140 (MQ=255)

               ccACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACTAATGGAGTGGCTCTGct                                                                                                                    >  1:168070/1‑140 (MQ=255)

               ccACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGct                                                                                                                    >  1:27898/1‑140 (MQ=255)

                       ccGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTg                                                                                                            >  2:155170/1‑140 (MQ=255)

                        cGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCAGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGt                                                                                                           <  1:78693/140‑1 (MQ=255)

                                 cATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACa                                                                                                  <  2:193084/140‑1 (MQ=255)

                                  aTTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCACAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACaa                                                                                                 >  2:365333/1‑140 (MQ=255)

                                      aCTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGAt                                                                                             <  1:409194/140‑1 (MQ=255)

                                           gTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACaa                                                                                        >  1:419830/1‑140 (MQ=255)

                                                    cGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACaa                                                                                                 <  2:463201/122‑1 (MQ=255)

                                                    cGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACaa                                                                                                 >  1:463201/1‑122 (MQ=255)

                                                    cGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGa                                                                               >  1:4640/1‑140 (MQ=255)

                                                     gTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGaa                                                                              <  2:27898/140‑1 (MQ=255)

                                                        cTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAAcgc                                                                           <  1:155170/140‑1 (MQ=255)

                                                           aCAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAAcgccgc                                                                        <  2:60932/140‑1 (MQ=255)

                                                             aaCGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTa                                                                      <  1:10551/140‑1 (MQ=255)

                                                             aaCGTGATCATCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAAcgccg                                                                         >  1:73816/1‑137 (MQ=255)

                                                             aaCGTGATCATCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAAcgccg                                                                         <  2:73816/137‑1 (MQ=255)

                                                                gTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTa                                                                   <  2:165585/140‑1 (MQ=255)

                                                                         aTCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCt                                                          >  1:479123/1‑140 (MQ=255)

                                                                               tACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGa                                                    <  2:4640/140‑1 (MQ=255)

                                                                                 cGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAat                                                  >  2:288/1‑140 (MQ=255)

                                                                                  gCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTc                                                           >  2:110978/1‑130 (MQ=255)

                                                                                  gCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTc                                                           <  1:110978/130‑1 (MQ=255)

                                                                                  gCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAata                                                 >  1:512897/1‑140 (MQ=255)

                                                                                                   gCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTcccggc                                <  2:168676/140‑1 (MQ=255)

                                                                                                                     aCCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTCCTTCCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGGCAACGGGCCGGAtt              <  2:416612/140‑1 (MQ=255)

                                                                                                                     aCCGTTCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGGCAACGGGCCGGAtt              <  1:365333/140‑1 (MQ=255)

                                                                                                                         ttCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACg          >  2:427077/1‑140 (MQ=255)

                                                                                                                         ttCCTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACg          >  2:493311/1‑140 (MQ=255)

                                                                                                                           ccTGATGTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCa        <  1:48488/140‑1 (MQ=255)

                                                                                                                                 gTTCGTACCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGt  >  2:384340/1‑140 (MQ=255)

CAATGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACGCCGAAATTTCGCAAGGGCTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTGTCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAATCCCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGT  >  NC_000913/3502065‑3502333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A