Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R1
|
139 |
58.0 |
1973578 |
98.9% |
1951868 |
138.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,727,494 |
(G)6→8 |
coding (836/1098 nt) |
nemA → |
N‑ethylmaleimide reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,727,488 | 1 | . | G | 100.0%
| 168.5
/ NA
| 47 | A277G (GCG→GGG) | nemA | N‑ethylmaleimide reductase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (25/22); total (25/22) |
| * | NC_000913 | 1,727,488 | 2 | . | G | 100.0%
| 167.4
/ NA
| 47 | A277G (GCG→GGG) | nemA | N‑ethylmaleimide reductase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (25/22); total (25/22) |
TGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCG > NC_000913/1727351‑1727618
||
tGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑‑gg < 2:176772/140‑1 (MQ=255)
tGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGACGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑‑gg < 2:984283/140‑1 (MQ=255)
gCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑‑gggg < 2:904630/140‑1 (MQ=255)
gCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑‑gggg < 2:353200/140‑1 (MQ=255)
cgcgTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑‑ggggggggt < 1:50216/140‑4 (MQ=255)
gcgTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑‑gggggggggt < 1:511206/140‑5 (MQ=255)
gTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑‑ggggggggtgaa < 2:756768/140‑7 (MQ=255)
tCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTg > 1:455946/1‑140 (MQ=255)
tACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGc > 2:765375/1‑140 (MQ=255)
cacaGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGaaaa > 1:432007/1‑140 (MQ=255)
aGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGt > 1:685235/1‑140 (MQ=255)
aCGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTAcgcg < 1:765375/140‑1 (MQ=255)
ggCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGcc < 2:455946/140‑1 (MQ=255)
ccGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGt < 2:773352/140‑1 (MQ=255)
tGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCa < 1:747163/140‑1 (MQ=255)
gCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCg > 2:724183/1‑140 (MQ=255)
ccGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCAc < 1:892625/133‑1 (MQ=255)
ccGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCAc > 2:892625/1‑133 (MQ=255)
tGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCATAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGgcg < 1:199418/140‑1 (MQ=255)
tGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGgcg < 2:432007/140‑1 (MQ=255)
tATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCa < 1:190558/140‑1 (MQ=255)
tATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCa < 1:656977/140‑1 (MQ=255)
aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCa < 1:849725/130‑1 (MQ=255)
aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCa > 2:849725/1‑130 (MQ=255)
aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATaca > 1:802428/1‑140 (MQ=255)
aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATaca > 1:250912/1‑140 (MQ=255)
aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATaca > 1:229727/1‑140 (MQ=255)
aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATaca > 2:123619/1‑140 (MQ=255)
aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATaca > 1:586844/1‑140 (MQ=255)
aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATaca > 2:280102/1‑140 (MQ=255)
caacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATAcaca < 1:591409/140‑1 (MQ=255)
gggTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGa < 1:426820/140‑1 (MQ=255)
tAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGaaaa < 1:724183/140‑1 (MQ=255)
aCGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGc > 2:687419/1‑140 (MQ=255)
gcgGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTg > 1:707343/1‑140 (MQ=255)
gCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGaaa > 2:401185/1‑140 (MQ=255)
cATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAAc > 2:977110/1‑140 (MQ=255)
cTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTACACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGa > 2:35690/1‑140 (MQ=255)
tATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATc < 1:280102/140‑1 (MQ=255)
cTGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGc > 1:535889/1‑140 (MQ=255)
tGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCa < 2:229727/140‑1 (MQ=255)
tGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCa < 1:123619/140‑1 (MQ=255)
tGCATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTACACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCa < 1:35690/140‑1 (MQ=255)
cATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTg < 1:62065/123‑1 (MQ=255)
cATATGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTg > 2:62065/1‑123 (MQ=255)
tatGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAgg < 1:687419/140‑1 (MQ=255)
tatGTCAGAACCAGATTGGGCGGGGGGGGTGAACCATATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAgg < 2:685235/140‑1 (MQ=255)
atGTCAGAACCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAggg < 1:977110/140‑1 (MQ=255)
aaCCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAgg < 2:533665/132‑1 (MQ=255)
aaCCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAgg > 1:533665/1‑132 (MQ=255)
aaCCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTg > 2:586854/1‑140 (MQ=255)
aaCCAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTg > 2:507935/1‑140 (MQ=255)
cAGATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATg < 1:85579/140‑1 (MQ=255)
gATTGGGCGGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCg > 2:708796/1‑140 (MQ=255)
gATTGGGCGGGGGGGGGGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGACCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCg > 2:895685/1‑140 (MQ=255)
||
TGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCG > NC_000913/1727351‑1727618
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
