Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 139 58.0 1973578 98.9% 1951868 138.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,274,122 T→C W587R (TGG→CGG)  yejA → putative oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,274,1220TC100.0% 255.6 / NA 71W587R (TGG→CGG) yejAputative oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/30);  total (41/30)

CACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGT  >  NC_000913/2273988‑2274258
                                                                                                                                      |                                                                                                                                        
cACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTAt                                                                                                                                     <  2:139884/140‑1 (MQ=255)
     gATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGt                                                                                                                                >  1:188327/1‑140 (MQ=255)
       tCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCa                                                                                                                              <  1:707078/140‑1 (MQ=255)
          cgTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATa                                                                                                                           >  1:780749/1‑140 (MQ=255)
                 aCGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGc                                                                                                                    >  2:709004/1‑140 (MQ=255)
                    tGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCaa                                                                                                                 >  2:552016/1‑140 (MQ=255)
                      gAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAAc                                                                                                               >  2:928151/1‑140 (MQ=255)
                        attattACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTg                                                                                                             <  2:780749/140‑1 (MQ=255)
                             taCATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTc                                                                                                        >  2:417237/1‑140 (MQ=255)
                                 tgctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGcc                                                                                                    <  1:112405/140‑1 (MQ=255)
                                 tgctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGcc                                                                                                    >  2:963641/1‑140 (MQ=255)
                                 tgctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGcc                                                                                                    <  1:892431/140‑1 (MQ=255)
                                 tgctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGcc                                                                                                    >  1:472880/1‑140 (MQ=255)
                                   ctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAg                                                                                                  >  1:776929/1‑140 (MQ=255)
                                    tgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTc                                                                                                        <  1:134107/133‑1 (MQ=255)
                                    tgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTc                                                                                                        >  2:134107/1‑133 (MQ=255)
                                    tgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGc                                                                                                 >  1:985790/1‑140 (MQ=255)
                                     gcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCa                                                                                                <  1:417237/140‑1 (MQ=255)
                                      ccAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCaa                                                                                               >  2:201989/1‑140 (MQ=255)
                                        aaTGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAAc                                                                                             <  2:355082/140‑1 (MQ=255)
                                             ggTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGga                                                                                        <  2:985790/140‑1 (MQ=255)
                                                aCATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGagag                                                                                     <  2:188327/140‑1 (MQ=255)
                                                    ggCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGa                                                                                 >  2:249543/1‑140 (MQ=255)
                                                    ggCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGa                                                                                 >  2:536287/1‑140 (MQ=255)
                                                    ggCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGa                                                                                 >  1:622074/1‑140 (MQ=255)
                                                    ggCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGa                                                                                 >  1:747584/1‑140 (MQ=255)
                                                     gCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGAt                                                                                <  1:709004/140‑1 (MQ=255)
                                                        gAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATggg                                                                             >  1:270853/1‑140 (MQ=255)
                                                        gAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATggg                                                                             <  2:491353/140‑1 (MQ=255)
                                                              cGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTa                                                                       >  2:174072/1‑140 (MQ=255)
                                                                tctcGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTAcc                                                                     >  1:146869/1‑140 (MQ=255)
                                                                     ccTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGAtt                                                                >  2:537/1‑140 (MQ=255)
                                                                       tggtggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCg                                                              <  1:537/140‑1 (MQ=255)
                                                                       tggtggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCg                                                              <  1:536287/140‑1 (MQ=255)
                                                                          tggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCg                                                           >  1:735444/1‑140 (MQ=255)
                                                                            ggATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTc                                                         <  2:747584/140‑1 (MQ=255)
                                                                            ggATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGACACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTc                                                         <  1:916538/140‑1 (MQ=255)
                                                                               tAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGt                                                      <  2:776929/140‑1 (MQ=255)
                                                                                      tcCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                               <  1:963641/140‑1 (MQ=255)
                                                                                      tcCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                               <  2:622074/140‑1 (MQ=255)
                                                                                       cccAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTg                                              >  1:318946/1‑140 (MQ=255)
                                                                                       cccAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTg                                              >  1:790144/1‑140 (MQ=255)
                                                                                        ccAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGa                                             >  2:854458/1‑140 (MQ=255)
                                                                                            ccggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                         <  2:735444/140‑1 (MQ=255)
                                                                                              ggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCaa                                       <  1:547074/140‑1 (MQ=255)
                                                                                               gccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                               <  2:215590/131‑1 (MQ=255)
                                                                                               gccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                               >  1:215590/1‑131 (MQ=255)
                                                                                                ccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACa                                     <  1:928151/140‑1 (MQ=255)
                                                                                                 cgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                         >  2:815553/1‑135 (MQ=255)
                                                                                                 cgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                         <  1:815553/135‑1 (MQ=255)
                                                                                                   tGCGCCCCATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTa                                  <  1:249543/140‑1 (MQ=255)
                                                                                                       ccccATCTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg                              <  2:270853/140‑1 (MQ=255)
                                                                                                             cTATAGCCTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTa                        >  1:243565/1‑140 (MQ=255)
                                                                                                                   ccTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg                              <  1:894007/128‑1 (MQ=255)
                                                                                                                   ccTCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg                              >  2:894007/1‑128 (MQ=255)
                                                                                                                     tCGGTATCGATGCGTGGCGGTATGACGTCAATAAAGCGGCAAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                <  2:146869/140‑1 (MQ=255)
                                                                                                                     tCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                <  2:243565/140‑1 (MQ=255)
                                                                                                                     tCGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                >  1:26209/1‑140 (MQ=255)
                                                                                                                      cGGTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCa               >  2:704315/1‑140 (MQ=255)
                                                                                                                       ggTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTa                        <  2:891490/130‑1 (MQ=255)
                                                                                                                       ggTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTa                        >  1:891490/1‑130 (MQ=255)
                                                                                                                       ggTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCaa              >  2:61208/1‑140 (MQ=255)
                                                                                                                       ggTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCaa              >  2:425833/1‑140 (MQ=255)
                                                                                                                       ggTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCaa              >  1:238414/1‑140 (MQ=255)
                                                                                                                       ggTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCaa              >  1:717445/1‑140 (MQ=255)
                                                                                                                       ggTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCaa              >  2:341044/1‑140 (MQ=255)
                                                                                                                        gTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTa                        <  1:309696/129‑1 (MQ=255)
                                                                                                                        gTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTa                        >  2:309696/1‑129 (MQ=255)
                                                                                                                        gTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAc             >  2:638101/1‑140 (MQ=255)
                                                                                                                        gTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAc             >  2:128276/1‑140 (MQ=255)
                                                                                                                        gTATCGATACCTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAAc             >  2:399091/1‑140 (MQ=255)
                                                                                                                                 ccTGGCGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATc    >  1:928840/1‑140 (MQ=255)
                                                                                                                                   tggcggTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGt  >  2:949129/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                        
CACTGGATCGCGTATTAACGTGGAATTATTACATGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGT  >  NC_000913/2273988‑2274258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A