Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	139	58.0	1973578	98.9%	1951868	138.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,471,767	(C)_5→4	pseudogene (296/519 nt)	yfdL ←	CPS‑53 (KpLE1) prophage; putative uncharacterized protein YfdL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,471,763	0	C	.	100.0%	152.9 / NA	36	pseudogene (300/519 nt)	yfdL	CPS‑53 (KpLE1) prophage; putative uncharacterized protein YfdL
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (19/17); total (19/17)

AACCGGCACAAGCTATATAGCCAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGG  >  NC_000913/2471624‑2471898

aaCCGGCACAAGCTATATAGCCAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGc                                                                                                                                         >  2:706861/1‑140 (MQ=255)

     gCACAAGCTATATAGCCAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGccccat                                                                                                                                    >  2:364383/1‑138 (MQ=255)

     gCACAAGCTATATAGCCAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGccccat                                                                                                                                    >  1:932199/1‑138 (MQ=255)

                    ccAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGcccca                                                                                                                                     <  1:415140/124‑2 (MQ=255)

                    ccAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGcccca                                                                                                                                     >  2:415140/1‑123 (MQ=255)

                       aCATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGAt                                                                                                                 >  2:525330/1‑140 (MQ=255)

                       aCATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGAt                                                                                                                 >  1:660993/1‑140 (MQ=255)

                        cATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGa                                                                                                                                <  2:788160/124‑1 (MQ=255)

                        cATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGa                                                                                                                                >  1:788160/1‑124 (MQ=255)

                         aTTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTa                                                                                                                         <  1:745490/130‑1 (MQ=255)

                         aTTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTa                                                                                                                         >  2:745490/1‑130 (MQ=255)

                         aTTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATgg                                                                                                               <  2:660993/140‑1 (MQ=255)

                                   ccGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGa                                                                                                     <  2:773511/140‑1 (MQ=255)

                                   ccGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGa                                                                                                     >  2:778208/1‑140 (MQ=255)

                                        aGTGTAATCCTGAGTCGCAAATACACGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATcc                                                                                                >  1:246698/1‑140 (MQ=255)

                                         gtgtAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGCTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCa                                                                                               <  2:186547/140‑1 (MQ=255)

                                           gtAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAgg                                                                                             >  2:321660/1‑140 (MQ=255)

                                             aaTCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGACCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGaa                                                                                           >  1:861042/1‑140 (MQ=255)

                                               tCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACt                                                                                         >  2:533996/1‑140 (MQ=255)

                                                    aGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGc                                                                                    >  2:74085/1‑140 (MQ=255)

                                                    aGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGc                                                                                    >  2:824998/1‑140 (MQ=255)

                                                    aGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGc                                                                                    >  2:47752/1‑140 (MQ=255)

                                                           aaTACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGc                                                                             >  1:313222/1‑140 (MQ=255)

                                                            aTACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCa                                                                            <  1:47752/140‑1 (MQ=255)

                                                            aTACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCa                                                                            <  1:525330/140‑1 (MQ=255)

                                                                    aGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTa                                                                    <  1:628860/140‑1 (MQ=255)

                                                                    aGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTa                                                                    >  1:553165/1‑140 (MQ=255)

                                                                     gACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTAc                                                                   <  2:837206/140‑1 (MQ=255)

                                                                      aCATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTAcc                                                                  <  1:533996/140‑1 (MQ=255)

                                                                        aTCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCa                                                                <  2:861042/140‑1 (MQ=255)

                                                                         tCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCat                                                               <  2:553165/140‑1 (MQ=255)

                                                                          cTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCata                                                              <  2:932199/140‑1 (MQ=255)

                                                                          cTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑‑CCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCatat                                                             >  1:497372/1‑140 (MQ=255)

                                                                                            ggAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTCCCCATATCAGGTATCTGATTCGcc                                            <  2:766361/140‑1 (MQ=255)

                                                                                              aaaaTAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGcccc                                          <  2:313222/140‑1 (MQ=255)

                                                                                                   aGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTcc                                     <  2:246698/140‑1 (MQ=255)

                                                                                                                        tCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACa                                >  2:718446/1‑124 (MQ=255)

                                                                                                                        tCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACa                                <  1:718446/124‑1 (MQ=255)

                                                                                                                        tCAGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCtt                >  2:763307/1‑140 (MQ=255)

                                                                                                                          aGCGGAACATCAATTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTtct              >  2:771554/1‑140 (MQ=255)

                                                                                                                                      aTTGG‑CCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAgg  >  2:711538/1‑140 (MQ=255)

AACCGGCACAAGCTATATAGCCAACATTTGCACTGCCGGGAGTGTAATCCTGAGTCGCAAATACCCGCAGACATCTATTCGGAAATGCAATCGGAAAATAGGTTACTGTGTCCTGAGACGTCAGCGGAACATCAATTGGCCCCCATTGAATAATTAAACCGGATGGCAATTTTTGATATCCAGGAACTGAAGCAGAAAGCATAAAACTACCCATATCAGGTATCTGATTCGCCCCTGTCCCTACATTTCTTTTAGCCGCTTCTCCCAAACCAAGG  >  NC_000913/2471624‑2471898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A