Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	139	58.0	1973578	98.9%	1951868	138.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,711,316	T→C	H299H (CAT→CAC)	nadB →	L‑aspartate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,711,316	0	T	C	100.0%	147.5 / NA	42	H299H (CAT→CAC)	nadB	L‑aspartate oxidase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/18); total (24/18)

GCAATTTCCTGTTAACAGAAGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCATGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGATCTCACACAAGAACCGGTA  >  NC_000913/2711180‑2711451

gCAATTTCCTGTTAACAGAAGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCacgaa                                                                                                                                      >  1:19311/1‑140 (MQ=255)

gCAATTTCCTGTTAACAGAAGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCacgaa                                                                                                                                      >  2:705165/1‑140 (MQ=255)

      tCCTGTTAACAGAAGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCAcgaaatgaaa                                                                                                                                <  1:705165/140‑1 (MQ=255)

          gTTAACAGAAGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGcc                                                                                                                            <  2:245015/140‑1 (MQ=255)

              aCAGAAGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCgg                                                                                                                        >  2:900083/1‑140 (MQ=255)

                aGAAGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGgcg                                                                                                                      <  2:577271/140‑1 (MQ=255)

                 gAAGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGgcgc                                                                                                                     <  2:592163/140‑1 (MQ=255)

                  aaGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGgcg                                                                                                                      >  1:337320/1‑138 (MQ=255)

                  aaGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGgcg                                                                                                                      <  2:337320/138‑1 (MQ=255)

                     cACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGAt                                                                                                                 >  2:738131/1‑140 (MQ=255)

                          cgcgGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTAt                                                                                                            >  1:43715/1‑140 (MQ=255)

                           gcggcgAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATg                                                                                                           <  1:812683/140‑1 (MQ=255)

                           gcggcgAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATg                                                                                                           <  2:43715/140‑1 (MQ=255)

                                gAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCt                                                                                                      <  1:955620/140‑1 (MQ=255)

                                  aGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTg                                                                                                    >  1:94786/1‑140 (MQ=255)

                                       cTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATc                                                                                               <  2:94786/140‑1 (MQ=255)

                                                    cccGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCccg                                                                                  >  2:86779/1‑140 (MQ=255)

                                                             tACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCTGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTa                                                                         >  1:314124/1‑140 (MQ=255)

                                                              aCGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTAt                                                                        >  1:762852/1‑140 (MQ=255)

                                                               cgcgTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTAtt                                                                       >  1:150778/1‑140 (MQ=255)

                                                                     ttATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAg                                                                 >  2:546693/1‑140 (MQ=255)

                                                                              aTTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCg                                                        >  1:376010/1‑140 (MQ=255)

                                                                                tttGATGAGCGCGGCGAACTGGCCCTGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCgat                                                      <  2:314124/140‑1 (MQ=255)

                                                                                         cgcgGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGa                                             >  1:849542/1‑140 (MQ=255)

                                                                                         cgcgGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGa                                             >  1:474394/1‑140 (MQ=255)

                                                                                         cgcgGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGa                                             >  1:573764/1‑140 (MQ=255)

                                                                                         cgcgGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGa                                             >  1:725220/1‑140 (MQ=255)

                                                                                           cggcgAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCgatgat                                                   <  2:715849/132‑1 (MQ=255)

                                                                                           cggcgAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCgatgat                                                   >  1:715849/1‑132 (MQ=255)

                                                                                                  cTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAgctgct                                    <  2:474394/140‑1 (MQ=255)

                                                                                                       cccGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGc                               <  2:376010/140‑1 (MQ=255)

                                                                                                        ccGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCt                              <  2:725220/140‑1 (MQ=255)

                                                                                                        ccGCGCGATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCt                              >  2:658012/1‑140 (MQ=255)

                                                                                                          gcgcgATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTgg                            >  2:960319/1‑140 (MQ=255)

                                                                                                             cgATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGa                         >  1:878934/1‑140 (MQ=255)

                                                                                                              gATATTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGAt                        <  1:279889/140‑1 (MQ=255)

                                                                                                               atatTGTCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGAtt                       >  2:658350/1‑140 (MQ=255)

                                                                                                                     tCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAtctc                 >  1:2135/1‑140 (MQ=255)

                                                                                                                     tCGCCCGCGCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGAtctc                 >  1:149033/1‑140 (MQ=255)

                                                                                                                          cgcgCCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGATCTcacaca            <  1:900083/140‑1 (MQ=255)

                                                                                                                            cgcCATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGATCTCACACAAg          >  1:83433/1‑140 (MQ=255)

                                                                                                                              ccATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGATCTCACACAAGaa        <  2:2135/140‑1 (MQ=255)

                                                                                                                              ccATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGATCTCACACAAGaa        <  2:149033/140‑1 (MQ=255)

                                                                                                                              ccATTGACCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGATCTCACACAAGaa        <  2:762852/140‑1 (MQ=255)

                                                                                                                                    aCCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGATCTCACACAAGAACCGGTa  <  1:738131/140‑1 (MQ=255)

                                                                                                                                    aCCACGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGATCTCACACAAGAACCGGTa  >  1:866574/1‑140 (MQ=255)

GCAATTTCCTGTTAACAGAAGCACTGCGCGGCGAAGGCGCTTATCTCAAGCGCCCGGATGGTACGCGTTTTATGCCCGATTTTGATGAGCGCGGCGAACTGGCCCCGCGCGATATTGTCGCCCGCGCCATTGACCATGAAATGAAACGCCTCGGCGCAGATTGTATGTTCCTTGATATCAGCCATAAGCCCGCCGATTTTATTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGGCTGGGGATTGATCTCACACAAGAACCGGTA  >  NC_000913/2711180‑2711451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A