Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 139 58.0 1973578 98.9% 1951868 138.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,817,885 G→A intergenic (‑25/+174) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,817,8850GA100.0% 178.9 / NA 50intergenic (‑25/+174)argQ/argZtRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/31);  total (19/31)

CCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTA  >  NC_000913/2817756‑2818019
                                                                                                                                 |                                                                                                                                       
ccTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCACTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATAc                                                                                                                               <  1:905422/140‑1 (MQ=255)
 cTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATAcc                                                                                                                              >  2:555670/1‑140 (MQ=255)
 cTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATAcc                                                                                                                              >  1:220499/1‑140 (MQ=255)
 cTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATAcc                                                                                                                              <  1:366455/140‑1 (MQ=255)
 cTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATAcc                                                                                                                              >  1:859474/1‑140 (MQ=255)
  ttCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCg                                                                                                                             <  1:880984/140‑1 (MQ=255)
  ttCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCg                                                                                                                             <  2:785126/140‑1 (MQ=255)
   tCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGc                                                                                                                            >  2:405934/1‑140 (MQ=255)
       tGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGctact                                                                                                                        <  2:910145/140‑1 (MQ=255)
       tGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGctact                                                                                                                        <  1:895960/140‑1 (MQ=255)
       tGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGctact                                                                                                                        <  1:773863/140‑1 (MQ=255)
                ttACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCata                                                                                                               <  1:124647/140‑1 (MQ=255)
                ttACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCata                                                                                                               <  1:866550/140‑1 (MQ=255)
                ttACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCata                                                                                                               <  1:555670/140‑1 (MQ=255)
                ttACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCata                                                                                                               <  1:454836/140‑1 (MQ=255)
                ttACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCata                                                                                                               <  1:405934/140‑1 (MQ=255)
                 tACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCatat                                                                                                              <  2:273826/140‑1 (MQ=255)
                 tACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCatat                                                                                                              <  1:227941/140‑1 (MQ=255)
                 tACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCatat                                                                                                              <  2:810908/140‑1 (MQ=255)
                          tatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAaga                                                                                                     >  1:810909/1‑140 (MQ=255)
                               tGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCatat                                                                                                              >  2:277880/1‑126 (MQ=255)
                               tGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCatat                                                                                                              >  2:821880/1‑126 (MQ=255)
                               tGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCatat                                                                                                              <  1:277880/126‑1 (MQ=255)
                               tGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCatat                                                                                                              <  1:821880/126‑1 (MQ=255)
                               tGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATT‑ATACCGCTACTAAAGCCATATCAATTAAGAGATGGt                                                                                               >  1:597948/1‑140 (MQ=255)
                                  aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCata                                                                                                               >  2:765213/1‑122 (MQ=255)
                                  aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCata                                                                                                               <  1:765213/122‑1 (MQ=255)
                                  aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGc                                                                                             >  1:635774/1‑140 (MQ=255)
                                    ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTTCTCATTCCAGTTGAGCTACGGCTGCATCGGGAAACCTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATTAATTAAGAGATGGCGCAt                                                                                           >  2:363364/1‑140 (MQ=14)
                                    ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCAt                                                                                           >  2:782964/1‑140 (MQ=255)
                                    ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATT‑ATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATc                                                                                          <  2:597948/140‑1 (MQ=255)
                                           gaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGggag                                                                                    <  1:888346/140‑1 (MQ=255)
                                                      ccGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGcc                                                                         <  2:810909/140‑1 (MQ=255)
                                                              tCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCt                                                                 >  1:657488/1‑140 (MQ=255)
                                                                ggTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCg                                                               <  2:635774/140‑1 (MQ=255)
                                                                ggTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCg                                                               <  1:566180/140‑1 (MQ=255)
                                                                                 tctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCt                                             <  2:657488/140‑1 (MQ=255)
                                                                                   tATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGTCCGTTgtaa                                            >  2:225656/1‑137 (MQ=255)
                                                                                      ccAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑cc                                                   <  2:98939/129‑1 (MQ=255)
                                                                                      ccAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑cc                                                   >  1:98939/1‑129 (MQ=255)
                                                                                       cAGCTGAGCTACGGATGCATCGGGAAAACTTATTCT‑CTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCaa                                      <  1:165295/140‑1 (MQ=255)
                                                                                          cTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACg                                    >  1:614242/1‑140 (MQ=255)
                                                                                            gAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGtt                                  <  2:212054/140‑1 (MQ=255)
                                                                                               cTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATGAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTatc                               >  2:878413/1‑140 (MQ=255)
                                                                                               cTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACg                                    >  2:209283/1‑135 (MQ=255)
                                                                                               cTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACg                                    <  1:209283/135‑1 (MQ=255)
                                                                                               cTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTat                                <  2:325862/139‑1 (MQ=255)
                                                                                               cTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTat                                >  1:325862/1‑139 (MQ=255)
                                                                                               cTACGGATGCATCGGGAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAACAACGTTatc                               >  1:180430/1‑140 (MQ=255)
                                                                                                            gggAAAACTTATTCTACTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCt                  <  1:878413/140‑1 (MQ=255)
                                                                                                                           aCTGCTAATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt   <  2:712733/140‑1 (MQ=255)
                                                                                                                            ctgctaATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt   >  2:135144/1‑139 (MQ=255)
                                                                                                                            ctgctaATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt   <  1:135144/139‑1 (MQ=255)
                                                                                                                            ctgctaATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTa  >  2:243386/1‑140 (MQ=255)
                                                                                                                            ctgctaATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTa  >  1:791011/1‑140 (MQ=255)
                                                                                                                                 |                                                                                                                                       
CCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGG‑CCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTA  >  NC_000913/2817756‑2818019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A