Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	139	58.0	1973578	98.9%	1951868	138.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,125,344	T→C	Q39R (CAG→CGG)	glcF ←	glycolate dehydrogenase, putative iron‑sulfur subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,125,344	0	T	C	100.0%	169.6 / NA	47	Q39R (CAG→CGG)	glcF	glycolate dehydrogenase, putative iron‑sulfur subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/23); total (24/23)

GGTGGTTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTC  >  NC_000913/3125208‑3125478

ggtggtTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGAt                                                                                                                                     >  2:718479/1‑140 (MQ=255)

ggtggtTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGAt                                                                                                                                     >  1:377350/1‑140 (MQ=255)

 gtggtTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATa                                                                                                                                    >  2:40481/1‑140 (MQ=255)

       tCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTgg                                                                                                                              <  2:17046/140‑1 (MQ=255)

        cacaATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTggg                                                                                                                             <  2:32610/140‑1 (MQ=255)

               aCGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTg                                                                                                                      >  1:156752/1‑140 (MQ=255)

                     aGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGt                                                                                                                                 >  1:625491/1‑123 (MQ=255)

                     aGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGt                                                                                                                                 <  2:625491/123‑1 (MQ=255)

                     aGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTAc                                                                                                                >  1:242093/1‑140 (MQ=255)

                     aGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTAc                                                                                                                >  1:480124/1‑140 (MQ=255)

                            cAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATcc                                                                                                         <  2:642983/140‑1 (MQ=255)

                             aGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCg                                                                                                        <  1:440729/140‑1 (MQ=255)

                             aGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCg                                                                                                        <  2:377350/140‑1 (MQ=255)

                                gATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAg                                                                                                     >  2:147912/1‑140 (MQ=255)

                                 aTCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGt                                                                                                    <  2:242093/140‑1 (MQ=255)

                                 aTCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATACGCCCGCGCGGCCCGTCCAGGTCAACGCCCAGAAGCCGATATGTTGGGCTGGTTTCTGTACAAAATCCGCAGt                                                                                                    <  2:828876/140‑1 (MQ=255)

                                            ccTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCa                                                                                                      <  1:516288/127‑1 (MQ=255)

                                            ccTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCa                                                                                                      >  2:516288/1‑127 (MQ=255)

                                            ccTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGgcg                                                                                         >  1:610871/1‑140 (MQ=255)

                                                     tAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATg                                                                                >  1:671660/1‑140 (MQ=255)

                                                      aaGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGc                                                                               <  1:623328/140‑1 (MQ=255)

                                                          gTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGgcg                                                                                         >  2:268113/1‑126 (MQ=255)

                                                          gTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGgcg                                                                                         <  1:268113/126‑1 (MQ=255)

                                                                     gCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcg                                                                <  2:406167/140‑1 (MQ=255)

                                                                           cAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAgcgcgcgcg                                                          >  1:71920/1‑140 (MQ=255)

                                                                              cACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGt                                                       <  1:718479/140‑1 (MQ=255)

                                                                                ccTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTc                                                     >  1:521670/1‑140 (MQ=255)

                                                                                ccTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTc                                                     <  2:458699/140‑1 (MQ=255)

                                                                                    tttAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGcc                                                 >  1:433874/1‑140 (MQ=255)

                                                                                    tttAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGcc                                                 >  1:401094/1‑140 (MQ=255)

                                                                                        aTCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCAt                                             <  2:521670/140‑1 (MQ=255)

                                                                                                  tGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGc                                               >  1:629494/1‑128 (MQ=255)

                                                                                                  tGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGc                                               <  2:629494/128‑1 (MQ=255)

                                                                                                   gcgcCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGc                                               <  1:575688/127‑1 (MQ=255)

                                                                                                   gcgcCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGc                                               >  2:575688/1‑127 (MQ=255)

                                                                                                     gcCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAAt                                <  2:156752/140‑1 (MQ=255)

                                                                                                     gcCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAAt                                <  2:671660/140‑1 (MQ=255)

                                                                                                     gcCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAAt                                <  1:40481/140‑1 (MQ=255)

                                                                                                     gcCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAAt                                >  1:351778/1‑140 (MQ=255)

                                                                                                      cccGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAAt                                <  2:947556/139‑1 (MQ=255)

                                                                                                      cccGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAAt                                >  1:947556/1‑139 (MQ=255)

                                                                                                       ccGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAAt                                <  1:901348/138‑1 (MQ=255)

                                                                                                       ccGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAAt                                >  2:901348/1‑138 (MQ=255)

                                                                                                         gcgcgGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTggg                            <  1:147912/140‑1 (MQ=255)

                                                                                                          cgcgGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTc                                       <  2:187605/128‑1 (MQ=255)

                                                                                                          cgcgGCCCGTCCAGTTCATCGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTc                                       >  1:187605/1‑128 (MQ=255)

                                                                                                                             cGCCCAGAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCa        >  1:488989/1‑140 (MQ=255)

                                                                                                                                   gAAGCCGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTc  >  1:361976/1‑140 (MQ=255)

GGTGGTTTCACAATTACGGCAAGTGAGGCAGCGATCGAGATGCTCCTGTGTTTTAAGCGTGACTTCGTTGCCTTCCAGCACCTGTTTAATCAGATAGATGCGCCCGCGCGGCCCGTCCAGTTCATCGCCCAGAAGCTGATAGGTTGGGCAGGTTGCGGTACAAAATCCGCAGTGAACACAGGCGCGCAGGATGCTGTCGGCTTCCAGCGCGCGCGCGTTCTGCCGCATCTCTTCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTC  >  NC_000913/3125208‑3125478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A