Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F28 I1 R1
|
176 |
31.5 |
1082462 |
98.8% |
1069472 |
138.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,700,566 |
G→A |
A404A (GCG→GCA) |
malX → |
PTS enzyme IIBC component MalX |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,700,566 | 0 | G | A | 100.0%
| 70.1
/ NA
| 21 | A404A (GCG→GCA) | malX | PTS enzyme IIBC component MalX |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/8); total (13/8) |
TTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGG > NC_000913/1700429‑1700700
|
ttAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTggcagc > 1:233419/1‑140 (MQ=255)
tAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGgcagca < 1:441706/140‑1 (MQ=255)
ccTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGt < 2:233419/140‑1 (MQ=255)
ccTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGt > 1:345198/1‑140 (MQ=255)
ccTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGt > 1:435290/1‑140 (MQ=255)
tgtgCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCt < 2:152772/140‑1 (MQ=255)
tgCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTc < 1:325225/140‑1 (MQ=255)
tgCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTc < 1:327607/140‑1 (MQ=255)
cGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGtt < 2:254786/140‑1 (MQ=255)
cACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGtt > 1:113043/1‑134 (MQ=255)
cACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGtt < 2:113043/134‑1 (MQ=255)
aCGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTg > 2:425520/1‑140 (MQ=255)
atatCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAAcc > 2:126918/1‑140 (MQ=255)
cGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATa > 2:439988/1‑140 (MQ=255)
cGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATa > 1:350645/1‑140 (MQ=255)
tgtTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGaa < 2:345198/140‑1 (MQ=255)
cAAGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTg > 1:416000/1‑140 (MQ=255)
aaGTGGTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGc > 1:23578/1‑140 (MQ=255)
ggTACATGGTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGt > 2:19438/1‑140 (MQ=255)
gTGCCAGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCggg > 1:152120/1‑140 (MQ=255)
ccAGTGGTGGGAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTaa > 2:446843/1‑140 (MQ=255)
aGTGGTGGCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAAt < 1:392647/140‑1 (MQ=255)
gtggCAGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAgg > 1:97760/1‑140 (MQ=255)
|
TTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGG > NC_000913/1700429‑1700700
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
