Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F28 I1 R1
|
176 |
31.5 |
1082462 |
98.8% |
1069472 |
138.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,465,426 |
G→A |
E42E (GAG→GAA) |
yfdC → |
inner membrane protein YfdC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,465,426 | 0 | G | A | 100.0%
| 84.3
/ NA
| 25 | E42E (GAG→GAA) | yfdC | inner membrane protein YfdC |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (10/15); total (10/15) |
GAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGA > NC_000913/2465295‑2465554
|
gagaCCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCg > 2:401015/1‑140 (MQ=255)
ggACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATg > 1:190606/1‑140 (MQ=255)
cGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACata < 2:535014/122‑1 (MQ=255)
cGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACata > 1:535014/1‑122 (MQ=255)
cGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGaaa > 1:299504/1‑140 (MQ=255)
ttGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTg < 2:339243/140‑1 (MQ=255)
aGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAagag < 1:393940/126‑1 (MQ=255)
aGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAagag > 2:393940/1‑126 (MQ=255)
aGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGc > 1:301553/1‑140 (MQ=255)
aaaTTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGgcg > 2:420589/1‑140 (MQ=255)
aaaTTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGgcg < 1:401015/140‑1 (MQ=255)
aaaTTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGgcg < 1:429027/140‑1 (MQ=255)
aaaTTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGgcg < 2:363456/140‑1 (MQ=255)
aaaTTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGgcg < 2:312782/140‑1 (MQ=255)
gAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACt < 2:299828/140‑1 (MQ=255)
gagaGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTc < 1:230469/140‑1 (MQ=255)
agCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGt < 2:440227/140‑1 (MQ=255)
cgcgGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGGCt > 2:353215/1‑140 (MQ=255)
cgcgGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACAGTGGTCAGCCATTGCGGCGGGTCt > 2:245336/1‑140 (MQ=255)
cAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACAGTGGTCAGCCATTGCGGCGGGTCTGTCGa < 1:245336/140‑1 (MQ=255)
aatagaaatagaTGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGgcg < 1:93488/140‑1 (MQ=255)
tGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACTgg < 2:179299/140‑1 (MQ=255)
ccGGGCGATGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAg < 2:299504/140‑1 (MQ=255)
tGGCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGGGCTTCGTTACTGGCAAAAAGGAGATTTTAAATCGAACTg > 1:396039/1‑140 (MQ=255)
gCAATTCATGAACATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGa < 1:36494/140‑1 (MQ=255)
|
GAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGA > NC_000913/2465295‑2465554
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
