Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 176 31.5 1082462 98.8% 1069472 138.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,795,349 G→A S366N (AGC→AAC)  gabP → 4‑aminobutyrate:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,795,3490GA100.0% 88.8 / NA 27S366N (AGC→AAC) gabP4‑aminobutyrate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/9);  total (18/9)

AATCAACCGCAGTAAAACCCCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATC  >  NC_000913/2795233‑2795485
                                                                                                                    |                                                                                                                                        
aaTCAACCGCAGTAAAACCCCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCctgct                                                                                                                     <  2:421555/138‑1 (MQ=255)
aaTCAACCGCAGTAAAACCCCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCctgct                                                                                                                     >  1:421555/1‑138 (MQ=255)
aaTCAACCGCAGTAAAACCCCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCctg                                                                                                                       <  1:17551/136‑1 (MQ=255)
aaTCAACCGCAGTAAAACCCCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCctg                                                                                                                       >  2:17551/1‑136 (MQ=255)
aaTCAACCGCAGTAAAACCCCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTgg                                                                                                                   >  1:317963/1‑140 (MQ=255)
                                tgtTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCgg                                                                                   >  2:416088/1‑140 (MQ=255)
                                       ctcCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCa                                                                            >  1:149712/1‑140 (MQ=255)
                                            ccGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAAtt                                                                       >  2:415353/1‑140 (MQ=255)
                                               gcgcGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTg                                                                    >  1:476901/1‑140 (MQ=255)
                                                  cgGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGa                                                                 >  1:46360/1‑140 (MQ=255)
                                                                    tggtgAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAAtt                                               >  1:80348/1‑140 (MQ=255)
                                                                    tggtgAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAAtt                                               >  2:73761/1‑140 (MQ=255)
                                                                    tggtgAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAAtt                                               >  2:427012/1‑140 (MQ=255)
                                                                     ggtgAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTc                                              <  2:80348/140‑1 (MQ=255)
                                                                        gAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCt                                           <  1:13878/140‑1 (MQ=255)
                                                                               tACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAAt                                    <  1:427012/140‑1 (MQ=255)
                                                                                   cgcCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTgg                                >  1:255676/1‑140 (MQ=255)
                                                                                    gcCTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGc                               <  2:220277/140‑1 (MQ=255)
                                                                                      cTGCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCtt                             >  2:157398/1‑140 (MQ=255)
                                                                                        gCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTa                           >  2:213558/1‑140 (MQ=255)
                                                                                        gCGAAGGTATTTAAATTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTa                           >  2:399350/1‑140 (MQ=255)
                                                                                                      aTTTCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCt             >  1:354645/1‑140 (MQ=255)
                                                                                                         tCTGATCGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGc          >  1:29633/1‑140 (MQ=255)
                                                                                                               cGACAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCa    >  1:175126/1‑140 (MQ=255)
                                                                                                                 aCAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATc  <  2:21729/140‑1 (MQ=255)
                                                                                                                 aCAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATc  <  2:476901/140‑1 (MQ=255)
                                                                                                                 aCAACTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATc  <  1:157398/140‑1 (MQ=255)
                                                                                                                    |                                                                                                                                        
AATCAACCGCAGTAAAACCCCGTACGTGGCGGTGTTACTCTCCACCGGCGCGGCATTCTTAACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTATTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGGTTTATTTAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATC  >  NC_000913/2795233‑2795485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A