Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F28 I1 R1
|
176 |
31.5 |
1082462 |
98.8% |
1069472 |
138.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,821,424 |
G→C |
G196G (GGC→GGG) |
alaS ← |
alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,821,424 | 0 | G | C | 90.0%
| 59.7
/ 0.2
| 20 | G196G (GGC→GGG) | alaS | alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base C (10/8); total (11/9) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 |
TACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACG > NC_000913/2821297‑2821555
|
tACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAAtg > 1:527938/1‑140 (MQ=255)
tACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGCGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAAtg > 2:197649/1‑140 (MQ=255)
ccGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGGGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAAtgtg < 2:535210/140‑1 (MQ=255)
aCGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGcc > 2:145154/1‑133 (MQ=255)
aCGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGcc < 1:145154/133‑1 (MQ=255)
cGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGt > 1:322002/1‑140 (MQ=255)
cATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACg < 2:527938/140‑1 (MQ=255)
ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa < 2:113312/140‑1 (MQ=255)
ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa > 1:113312/1‑140 (MQ=255)
ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa < 1:130212/140‑1 (MQ=255)
ggCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAc < 2:44082/140‑1 (MQ=255)
ccTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCa < 2:322002/140‑1 (MQ=255)
gATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGt > 1:163608/1‑140 (MQ=255)
ctcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATg > 2:497896/1‑140 (MQ=255)
aaTGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGA‑GGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGgcgc > 2:370647/1‑140 (MQ=255)
cGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCg > 1:260815/1‑126 (MQ=255)
cGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCg < 2:260815/126‑1 (MQ=255)
cGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCttt > 2:210010/1‑140 (MQ=255)
tcttcCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGAt < 2:163608/140‑1 (MQ=255)
gggCTTCCCGCA‑GGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGaata < 1:370647/140‑1 (MQ=255)
cccGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACg > 1:537420/1‑140 (MQ=255)
|
TACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACG > NC_000913/2821297‑2821555
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
