Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	176	31.5	1082462	98.8%	1069472	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,042,241	A→G	D310G (GAC→GGC)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,042,241	0	A	G	100.0%	92.9 / NA	27	D310G (GAC→GGC)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (11/16); total (11/16)

AGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTAT  >  NC_000913/3042107‑3042372

agagCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATg                                                                                                                                >  2:75714/1‑140 (MQ=255)

agagCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATg                                                                                                                                >  2:262506/1‑140 (MQ=255)

agagCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATg                                                                                                                                >  1:431712/1‑140 (MQ=255)

   gCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGa                                                                                                                             <  2:206459/140‑1 (MQ=255)

   gCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGa                                                                                                                             <  1:75714/140‑1 (MQ=255)

        aaaTGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACa                                                                                                                        <  2:202736/140‑1 (MQ=255)

         aaTGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACAt                                                                                                                       <  1:262506/140‑1 (MQ=255)

                     cTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGc                                                                                                           <  2:431712/140‑1 (MQ=255)

                     cTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGc                                                                                                           <  2:140842/140‑1 (MQ=255)

                                 cggtacggtaCTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTAtt                                                                                               <  2:98147/140‑1 (MQ=255)

                                         tacTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCTGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGaa                                                                                       >  2:9585/1‑140 (MQ=255)

                                               cTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTaa                                                                                 <  2:112951/140‑1 (MQ=255)

                                                 gCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAAt                                                                               <  1:533477/140‑1 (MQ=255)

                                                  cGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAAtc                                                                              <  2:243468/140‑1 (MQ=255)

                                                   ggTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTaa                                                                                 >  2:228333/1‑136 (MQ=255)

                                                   ggTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTaa                                                                                 <  1:228333/136‑1 (MQ=255)

                                                   ggTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAAtct                                                                             >  2:537236/1‑140 (MQ=255)

                                                                  tGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAgg                                                              <  1:403213/140‑1 (MQ=255)

                                                                                        gtcATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGc                                        >  1:191643/1‑140 (MQ=255)

                                                                                               aTAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGcgcg                                 >  2:418730/1‑140 (MQ=255)

                                                                                                    gATATGGAACCGGATAGCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTa                            >  2:155041/1‑140 (MQ=255)

                                                                                                       atGGAACCGGATAGCATCTTCCGCGTACGCGGCGATACGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCa                         >  2:386358/1‑140 (MQ=255)

                                                                                                                    gCATCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTg            <  1:537236/140‑1 (MQ=255)

                                                                                                                      aTCTTCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGAt          <  2:347906/140‑1 (MQ=255)

                                                                                                                          tCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGcc       <  1:383077/139‑1 (MQ=255)

                                                                                                                          tCCGCGTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGcc       >  2:383077/1‑139 (MQ=255)

                                                                                                                              cgTACGCGGCGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTAt  <  2:388149/140‑1 (MQ=255)

AGAGCTGAAAATGGGCGAGAACTGGCGTCGTACCGGTACGGTACTGGCTGCGGTAAAACTGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTAT  >  NC_000913/3042107‑3042372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A