Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	176	31.5	1082462	98.8%	1069472	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,052,056	T→C	S88G (AGC→GGC)	ubiI ←	2‑octaprenylphenol hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,052,056	0	T	C	100.0%	106.7 / NA	30	S88G (AGC→GGC)	ubiI	2‑octaprenylphenol hydroxylase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/17); total (13/17)

CGGGGGCTAACAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCTGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATT  >  NC_000913/3051921‑3052191

cGGGGGCTAACAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCt                                                                                                                                     <  2:251480/140‑1 (MQ=255)

 gggggCTAACAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCtt                                                                                                                                    <  2:319739/140‑1 (MQ=255)

      cTAACAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTcc                                                                                                                               <  1:290427/140‑1 (MQ=255)

      cTAACAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTcc                                                                                                                               <  1:506518/140‑1 (MQ=255)

        aaCAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCca                                                                                                                             <  2:82916/140‑1 (MQ=255)

          cAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCcaca                                                                                                                           <  1:492300/140‑1 (MQ=255)

               tGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCca                                                                                                                             >  1:239962/1‑133 (MQ=255)

               tGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCca                                                                                                                             <  2:239962/133‑1 (MQ=255)

               tGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTcc                                                                                                                      >  2:439301/1‑140 (MQ=255)

                           acTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATaa                                                                                                          >  1:47741/1‑140 (MQ=255)

                               aTGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGc                                                                                                      >  1:441845/1‑140 (MQ=255)

                                    cTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGcc                                                                                                 >  2:97309/1‑140 (MQ=255)

                                       tGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTa                                                                                              <  1:97309/140‑1 (MQ=255)

                                       tGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTa                                                                                              >  1:486208/1‑140 (MQ=255)

                                         ttCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACg                                                                                            >  2:332003/1‑140 (MQ=255)

                                              caGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACgagaga                                                                                       <  2:486208/140‑1 (MQ=255)

                                                 cgcgTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGaa                                                                                    >  1:308874/1‑140 (MQ=255)

                                                 cgcgTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGaa                                                                                    >  2:55682/1‑140 (MQ=255)

                                                           aTCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCa                                                                          <  2:257653/140‑1 (MQ=255)

                                                           aTCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCa                                                                          <  1:439301/140‑1 (MQ=255)

                                                              aCTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGAc                                                                       <  2:417114/140‑1 (MQ=255)

                                                                tGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGc                                                                     >  2:225830/1‑140 (MQ=255)

                                                                          aCGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGt                                                           <  1:55682/140‑1 (MQ=255)

                                                                             atatGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAg                                                        <  2:308874/140‑1 (MQ=255)

                                                                                          ggCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTg                                           <  1:332003/140‑1 (MQ=255)

                                                                                              ataGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTggcgg                                       <  2:441845/140‑1 (MQ=255)

                                                                                               taGCCCATGCTTTGATCGTCAAACGAAATGGGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCGCTACGAGAGAGAATGCCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTggcggc                                      >  2:499639/1‑140 (MQ=255)

                                                                                                                  cAAACGAAATGTGACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACgcgc                   >  2:247404/1‑140 (MQ=255)

                                                                                                                              gACCAAAGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTgtggtggt       >  2:228180/1‑140 (MQ=255)

                                                                                                                                   aaGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCAtt  >  2:362738/1‑140 (MQ=255)

                                                                                                                                   aaGCCGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCAtt  <  1:247404/140‑1 (MQ=255)

CGGGGGCTAACAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCTGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATT  >  NC_000913/3051921‑3052191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A