Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	176	31.5	1082462	98.8%	1069472	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,052,577	A→C	T314T (ACT→ACG)	ubiH ←	2‑octaprenyl‑6‑methoxyphenol hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,052,577	0	A	C	100.0%	105.7 / NA	30	T314T (ACT→ACG)	ubiH	2‑octaprenyl‑6‑methoxyphenol hydroxylase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (16/14); total (16/14)

GGTGCCCAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTA  >  NC_000913/3052440‑3052710

ggTGCCCAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGt                                                                                                                                     >  1:498657/1‑140 (MQ=255)

 gTGCCCAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTc                                                                                                                                    <  1:88550/140‑1 (MQ=255)

     ccAACAGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAggg                                                                                                                                >  1:364438/1‑140 (MQ=255)

      cAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGt                                                                                                                               <  2:498657/140‑1 (MQ=255)

            ttGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGc                                                                                                                         >  2:143570/1‑140 (MQ=255)

             tGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCa                                                                                                                        <  2:133798/140‑1 (MQ=255)

                        tGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATcac                                                                                                             >  2:210964/1‑140 (MQ=255)

                         gTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATcaca                                                                                                            >  1:202360/1‑140 (MQ=255)

                                    tCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATAcc                                                                                                 <  1:111266/140‑1 (MQ=255)

                                    tCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATAcc                                                                                                 >  2:111266/1‑140 (MQ=255)

                                    tCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATAcc                                                                                                 >  1:477144/1‑140 (MQ=255)

                                           cGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTa                                                                                          <  2:364438/140‑1 (MQ=255)

                                                aTGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAAccc                                                                                     >  2:392141/1‑140 (MQ=255)

                                                     tGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGcc                                                                                >  1:439901/1‑140 (MQ=255)

                                                              aTCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCg                                                                       <  2:439901/140‑1 (MQ=255)

                                                                                     aaCGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTg                                                <  1:392141/140‑1 (MQ=255)

                                                                                          cacaATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCccac                                           >  2:505632/1‑140 (MQ=255)

                                                                                          cacaATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCccac                                           >  2:225792/1‑140 (MQ=255)

                                                                                                    cGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTa                                 >  1:408618/1‑140 (MQ=255)

                                                                                                    cGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTa                                 >  1:108061/1‑140 (MQ=255)

                                                                                                      tAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACg                               <  1:143570/140‑1 (MQ=255)

                                                                                                      tAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACg                               <  2:202360/140‑1 (MQ=255)

                                                                                                      tAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACg                               <  1:505632/140‑1 (MQ=255)

                                                                                                       aaTCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGa                              <  1:225792/140‑1 (MQ=255)

                                                                                                       aaTCCCCCATGTCTTCTCCGCGCTCCTGCGCCCGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTACAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGa                              <  2:477144/140‑1 (MQ=255)

                                                                                                                    ttctCCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATc                 >  1:358131/1‑140 (MQ=255)

                                                                                                                       tcCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGAt                             <  2:213746/125‑1 (MQ=255)

                                                                                                                       tcCGCGCTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGAt                             >  1:213746/1‑125 (MQ=255)

                                                                                                                            gcTCCTGCGCCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGgcggcg         <  2:408618/140‑1 (MQ=255)

                                                                                                                                   cgcCTGCGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTa  >  1:144907/1‑140 (MQ=255)

GGTGCCCAACGGTTGGCAAAAAGATGTACAAGGCTGTCCGTGACGCCAATGGTTGCTTCGCGATCGCTCTGTCGACGCTGCTGATAACGGCACAATACGCCGTAATCCCCCATGTCTTCTCCGCGCTCCTGCGCCTGAGTCAGGGTTTCCGCAAGACTCATCACATCTCGCATACCGAGGTTAAACCCTTGCCCGGCAATCGGGTGCAGAGTTTGCGCCGCATTGCCCACCAGCACGGTACGATGGGTAATAGATCTGGCGGCGTGGGTTA  >  NC_000913/3052440‑3052710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A