Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 176 31.5 1082462 98.8% 1069472 138.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,089,159 (G)5→6 coding (876/1395 nt) galP → galactose:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,089,1541.G95.7% 160.4 / ‑0.3 47coding (871/1395 nt)galPgalactose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base G (25/20);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑T‑GGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACT  >  NC_000913/3089017‑3089284
                                                                                                                                           |                                                                                                                                  
aCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑T‑gg                                                                                                                                  >  1:247240/1‑140 (MQ=255)
aCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑T‑gg                                                                                                                                  >  1:388202/1‑140 (MQ=255)
  aGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGGGCAAATG‑T‑gggg                                                                                                                                >  2:320367/1‑140 (MQ=255)
              cgcgcgGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTg                                                                                                                     <  2:247240/140‑1 (MQ=255)
                                              aaTGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATc                                                                                     >  2:318598/1‑140 (MQ=255)
                                                tGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGc                                                                                   >  1:228020/1‑140 (MQ=255)
                                                tGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGc                                                                                   >  1:234622/1‑140 (MQ=255)
                                                tGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGc                                                                                   >  2:444692/1‑140 (MQ=255)
                                                 gcagcaATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCa                                                                                  <  1:318598/140‑1 (MQ=255)
                                                   agcaATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAAt                                                                                <  2:85816/140‑1 (MQ=255)
                                                   agcaATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAAt                                                                                <  2:234622/140‑1 (MQ=255)
                                                      aaTTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCgg                                                                             >  1:147011/1‑140 (MQ=255)
                                                        ttCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGcc                                                                           <  2:388202/140‑1 (MQ=255)
                                                              gggATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAAt                                                                                <  1:74309/129‑1 (MQ=255)
                                                              gggATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAAt                                                                                >  2:74309/1‑129 (MQ=255)
                                                                              tGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGGGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTaa                                                     >  1:310683/1‑140 (MQ=255)
                                                                                   tACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaa                                                >  1:418535/1‑140 (MQ=255)
                                                                                   tACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaa                                                >  1:144662/1‑140 (MQ=255)
                                                                                      gcgcCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgc                                             <  2:36564/140‑1 (MQ=255)
                                                                                       cgcCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgct                                            >  1:357859/1‑140 (MQ=255)
                                                                                       cgcCGAAAATCTTCGAACTGGCGGGTTATA‑CAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgcta                                           >  2:138515/1‑140 (MQ=255)
                                                                                          cGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGTTTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgctaac                                         >  1:499842/1‑140 (MQ=255)
                                                                                          cGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCaacgctaac                                         >  1:308216/1‑140 (MQ=255)
                                                                                               aTCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTgg                                    <  2:357859/140‑1 (MQ=255)
                                                                                                tCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTggg                                   <  2:329978/140‑1 (MQ=255)
                                                                                                tCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTggg                                   <  2:144662/140‑1 (MQ=255)
                                                                                                  ttCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCt                                 >  2:229950/1‑140 (MQ=255)
                                                                                                     gAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTcc                              <  2:147011/140‑1 (MQ=255)
                                                                                                          ggCGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTg                         <  2:228020/140‑1 (MQ=255)
                                                                                                            cGGGTTATACCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGAt                       <  1:436120/140‑1 (MQ=255)
                                                                                                                   taCCAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGgctgct                >  1:161474/1‑140 (MQ=255)
                                                                                                                     ccAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTgg              >  2:101968/1‑140 (MQ=255)
                                                                                                                     ccAACACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTgg              >  1:433726/1‑140 (MQ=255)
                                                                                                                       aaCACTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCa            <  1:138515/140‑1 (MQ=255)
                                                                                                                         cacTACCGAGCAAATGCG‑GGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATg          >  1:47485/1‑140 (MQ=255)
                                                                                                                         cacTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGgctgc                 >  1:427656/1‑133 (MQ=255)
                                                                                                                         cacTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGgctgc                 <  2:427656/133‑1 (MQ=255)
                                                                                                                         cacTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATg          >  2:69275/1‑140 (MQ=255)
                                                                                                                         cacTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATg          >  2:203217/1‑140 (MQ=255)
                                                                                                                           cTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATgg         >  2:369760/1‑139 (MQ=255)
                                                                                                                           cTACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATgg         <  1:369760/139‑1 (MQ=255)
                                                                                                                            tACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGc       <  1:444692/140‑1 (MQ=255)
                                                                                                                            tACCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGc       <  2:418535/140‑1 (MQ=255)
                                                                                                                             aCCGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCg      >  1:237902/1‑140 (MQ=255)
                                                                                                                              ccGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGt     >  2:52717/1‑140 (MQ=255)
                                                                                                                               cGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTa    <  1:52717/140‑1 (MQ=255)
                                                                                                                               cGAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTa    >  2:62049/1‑140 (MQ=255)
                                                                                                                                gAGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATgatccact   >  2:206304/1‑133 (MQ=255)
                                                                                                                                 aGCAAATGTT‑GGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACt  <  1:69275/140‑1 (MQ=255)
                                                                                                                                 aGCAAATG‑TGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACt  <  1:101968/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                  
ACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATG‑T‑GGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACT  >  NC_000913/3089017‑3089284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A