Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	176	31.5	1082462	98.8%	1069472	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,235,383	T→C	T165A (ACG→GCG)	rlmG ←	23S rRNA m(2)G1835 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,235,383	0	T	C	100.0%	143.6 / NA	40	T165A (ACG→GCG)	rlmG	23S rRNA m(2)G1835 methyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/17); total (23/17)

CGGGAGAAGACATTCGCATGGTTGTGGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAA  >  NC_000913/3235247‑3235518

cGGGAGAAGACATTCGCATGGTTGTGGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCgcggt                                                                                                                                      <  2:475815/140‑1 (MQ=255)

        gaCATTCGCATGGTTGTGGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTg                                                                                                                              >  1:139005/1‑140 (MQ=255)

        gaCATTCGCATGGTTGTGGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTg                                                                                                                              >  1:53525/1‑140 (MQ=255)

             tCGCATGGTTGTGGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCg                                                                                                                         >  2:140336/1‑140 (MQ=255)

                           aTAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGaa                                                                                                           <  2:113142/140‑1 (MQ=255)

                               tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCCATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGGGGTGGTTGGGCCGAGCACTTTTTCGAACAGt                                                                                                       >  2:228949/1‑140 (MQ=255)

                               tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGaa                                                                                                           >  1:8721/1‑136 (MQ=255)

                               tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGaa                                                                                                           <  2:8721/136‑1 (MQ=255)

                               tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGt                                                                                                       >  2:381265/1‑140 (MQ=255)

                               tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGt                                                                                                       >  2:18529/1‑140 (MQ=255)

                               tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGt                                                                                                       >  2:523473/1‑140 (MQ=255)

                               tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGt                                                                                                       >  1:531505/1‑140 (MQ=255)

                               tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGt                                                                                                       >  1:210578/1‑140 (MQ=255)

                               tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGt                                                                                                       >  1:148450/1‑140 (MQ=255)

                               tCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGt                                                                                                       >  1:101735/1‑140 (MQ=255)

                                 cagtcagtACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTc                                                                                                     <  2:446416/140‑1 (MQ=255)

                                 cagtcagtACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTc                                                                                                     <  1:140336/140‑1 (MQ=255)

                                 cagtcagtACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTc                                                                                                     <  2:210578/140‑1 (MQ=255)

                                       gtACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCactgt                                                                                               <  1:381265/140‑5 (MQ=255)

                                        tACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTg                                                                                              >  1:248806/1‑140 (MQ=255)

                                            ttccagcttccagcTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAg                                                                                          >  2:24642/1‑140 (MQ=255)

                                                                   gcggcgCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTT‑CATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCAc                                                                  >  2:332248/1‑140 (MQ=255)

                                                                    cggcgCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCAc                                                                  <  1:320327/140‑1 (MQ=255)

                                                                      gcgcATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCg                                                                >  2:141848/1‑140 (MQ=255)

                                                                       cgcATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCgg                                                               <  1:141848/140‑1 (MQ=255)

                                                                         cATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCa                                                             >  2:437052/1‑140 (MQ=255)

                                                                                 aGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACg                                                     <  2:53525/140‑1 (MQ=255)

                                                                                 aGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACg                                                     <  2:531505/140‑1 (MQ=255)

                                                                                              tGAAAGTGCAATTAATCAGGCGCGCTTTTTT‑CATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGa                                       <  1:332248/140‑1 (MQ=255)

                                                                                               gAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGa                                       <  2:248806/140‑1 (MQ=255)

                                                                                                aaaGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGAc                                      >  1:190694/1‑140 (MQ=255)

                                                                                                   gTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCAc                                   >  1:121327/1‑140 (MQ=255)

                                                                                                         ttAATCAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTgcg                             <  2:139005/140‑1 (MQ=255)

                                                                                                              cAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTgcgcagcg                        >  2:462445/1‑140 (MQ=255)

                                                                                                              cAGGCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTgcgcagcg                        >  1:260961/1‑140 (MQ=255)

                                                                                                                ggCGCGCTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTgcgcagcgca                      >  1:431441/1‑140 (MQ=255)

                                                                                                                 gcgcgcTTTTTTCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGcgcac                     <  2:34378/140‑1 (MQ=255)

                                                                                                                         ttttCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGc             <  2:260961/140‑1 (MQ=255)

                                                                                                                         ttttCCATGCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGc             <  2:101735/140‑1 (MQ=255)

                                                                                                                                 gCCAGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAg     >  2:538758/1‑140 (MQ=255)

                                                                                                                                    aGCGCGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCaa  <  1:462445/140‑1 (MQ=255)

CGGGAGAAGACATTCGCATGGTTGTGGATAGTCCAGTCAGTACCTTCCAGCTTCCAGCTAACGGTCTGCGGCGCATCGGCCAGCTGCGGTTCATTGAAAGTGCAATTAATCAGGCGCGCTTTTTTCCATGCCAGCGTGGTGGTGGTTGGGCCGAGCACTTTTTCGAACAGTTCCAGCGTGGAAGTGTGAATGTCACGGGCCTTGGCACCGGCAATAATACGTGTATCCGACGTGACCACTTTGCGCAGCGCACGCAGTTGCTGTTCCAGCAA  >  NC_000913/3235247‑3235518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A