Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	176	31.5	1082462	98.8%	1069472	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,756,167	G→A	P115P (CCC→CCT)	aldB ←	aldehyde dehydrogenase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,756,167	0	G	A	100.0%	105.0 / NA	31	P115P (CCC→CCT)	aldB	aldehyde dehydrogenase B
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/18); total (13/18)

ATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCATTTATCTTTCACTTTGTG  >  NC_000913/3756047‑3756298

aTAGGCCACGGTTTCGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGtt                                                                                                                                 >  2:448430/1‑125 (MQ=255)

aTAGGCCACGGTTTCGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGtt                                                                                                                                 <  1:448430/125‑1 (MQ=255)

    gCCACGGTTTCGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTc                                                                                                              >  2:419867/1‑140 (MQ=255)

     ccACGGTTTCGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCa                                                                                                             >  2:335004/1‑140 (MQ=255)

       aCGGTTTCGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGc                                                                                                           >  2:163579/1‑140 (MQ=255)

         ggTTTCGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTg                                                                                                         >  2:419228/1‑140 (MQ=255)

              cGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCt                                                                                                    >  1:43254/1‑140 (MQ=255)

                      aCTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTc                                                                                            <  1:532720/140‑1 (MQ=255)

                            cTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGtt                                                                                      <  2:147768/140‑1 (MQ=255)

                               aTCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTg                                                                                   <  1:419228/140‑1 (MQ=255)

                                         ccTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGtt                                                                                      <  1:20230/127‑1 (MQ=255)

                                         ccTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGtt                                                                                      >  2:20230/1‑127 (MQ=255)

                                         ccTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGa                                                                         >  1:328251/1‑140 (MQ=255)

                                           tGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATc                                                                       >  2:501745/1‑140 (MQ=255)

                                             cgcCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCgg                                                                     <  1:419867/140‑1 (MQ=255)

                                             cgcCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCgg                                                                     <  2:451879/140‑1 (MQ=255)

                                                       cacGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTaaa                                                           <  1:335004/140‑1 (MQ=255)

                                                                    aGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCa                                              <  1:418074/140‑1 (MQ=255)

                                                                         aaaTGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATc                                         <  1:163579/140‑1 (MQ=255)

                                                                         aaaTGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATc                                         <  1:32460/140‑1 (MQ=255)

                                                                           aTGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCa                                              >  2:206577/1‑133 (MQ=255)

                                                                           aTGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCa                                              <  1:206577/133‑1 (MQ=255)

                                                                           aTGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCt                                       <  2:129221/140‑1 (MQ=255)

                                                                                        aGCGGTACATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGtgtg                          <  1:501745/140‑1 (MQ=255)

                                                                                               cATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCAt                   <  1:484096/140‑1 (MQ=255)

                                                                                               cATCCGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCAt                   >  2:223424/1‑140 (MQ=255)

                                                                                                   cGCAGCACTGGTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCATTTAt               <  1:431626/140‑1 (MQ=255)

                                                                                                             gTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCATTTATCtt            >  2:388721/1‑133 (MQ=255)

                                                                                                             gTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCATTTATCtt            <  1:388721/133‑1 (MQ=255)

                                                                                                             gTTTCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCATTTATCTTTCACttt     <  2:43254/140‑1 (MQ=255)

                                                                                                                tCGCGAATAGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCATTTATCTTTCACTTtgtg  >  2:219473/1‑140 (MQ=255)

ATAGGCCACGGTTTCGCTATCAACTTCACTGATCCCACCTTCCTGCGCCCGAATACACGAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCATTTATCTTTCACTTTGTG  >  NC_000913/3756047‑3756298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A