Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	176	31.5	1082462	98.8%	1069472	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,809,588	C→T	P350L (CCG→CTG)	waaA →	KDO transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,809,588	0	C	T	100.0%	89.1 / NA	27	P350L (CCG→CTG)	waaA	KDO transferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/12); total (15/12)

GCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGT  >  NC_000913/3809456‑3809715

gCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACt                                                                                                                          >  1:89672/1‑140 (MQ=255)

           ggCGAGTTGATGTTACTGTATGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTaa                                                                                                               >  2:229289/1‑140 (MQ=255)

                      gTTACTGTATGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTgcg                                                                                                    <  2:89672/140‑1 (MQ=255)

                           tGTATGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCgg                                                                                               >  2:38986/1‑140 (MQ=255)

                              aTGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTg                                                                                            <  2:202981/140‑1 (MQ=255)

                                     tGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAgg                                                                                     >  1:238967/1‑140 (MQ=255)

                                     tGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAgg                                                                                     >  1:300122/1‑140 (MQ=255)

                                     tGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAgg                                                                                     >  2:151515/1‑140 (MQ=255)

                                       ccGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCa                                                                                   >  2:339713/1‑140 (MQ=255)

                                       ccGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCa                                                                                   >  2:249987/1‑140 (MQ=255)

                                       ccGATCTCGCCTTTGTTGGCGGTTCACTGGATGAACATGGTGGGCATAATCCGCTGGAAGCTGCCTCACACGCTATTCCGGTATTGATTGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCTGGCa                                                                                   >  2:239886/1‑140 (MQ=255)

                                                ccTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTg                                                                          <  1:229289/140‑1 (MQ=255)

                                                 cTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGa                                                                         >  1:16442/1‑140 (MQ=255)

                                                       tgGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGAttaccg                                                                   <  1:339713/140‑1 (MQ=255)

                                                           ggTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGAttaccgttac                                                               <  2:238967/140‑1 (MQ=255)

                                                           ggTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGAttaccgttac                                                               <  2:300122/140‑1 (MQ=255)

                                                            gTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGAttaccgttacc                                                              <  2:147915/140‑1 (MQ=255)

                                                                aCTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATg                                                          >  1:473077/1‑140 (MQ=255)

                                                                        aaCGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACg                                                  <  1:22070/140‑1 (MQ=255)

                                                                          cGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCt                                                <  1:249987/140‑1 (MQ=255)

                                                                          cGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTACTCCGGTATTGCTGGGGCTGCATACTTTTAACTTTAGAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATGACCGTTACCGATGCCACTACGCt                                                >  1:467004/1‑140 (MQ=255)

                                                                            tggtggGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTg                                              >  1:491241/1‑140 (MQ=255)

                                                                                            cTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCctc                              >  2:539721/1‑140 (MQ=255)

                                                                                                     gCCGCACACGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCAc                     <  1:371000/140‑1 (MQ=255)

                                                                                                            acGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGcc              <  1:151515/140‑1 (MQ=255)

                                                                                                            acGCTATTCCGGTATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGcc              <  2:126068/140‑1 (MQ=255)

                                                                                                                        tATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGt  >  1:172150/1‑140 (MQ=255)

                                                                                                                        tATTGATGGGGCTGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGt  >  1:509689/1‑140 (MQ=255)

GCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATACTTTTAACTTTAAAGACATTTGCGCGCGGCTGGAGCAGGCAAGCGGGCTGATTACCGTTACCGATGCCACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGT  >  NC_000913/3809456‑3809715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A