Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A26 F30 I1 R1 19 50.2 1707380 99.2% 1693720 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,359,023 C→A G377V (GGG→GTG)  yfaV ← putative transporter YfaV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,359,0230CA98.3% 196.0 / ‑3.9 58G377V (GGG→GTG) yfaVputative transporter YfaV
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (26/31);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GCAACAGTGCGATTTACACGCAGAGTGAAAATAATCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACG  >  NC_000913/2358888‑2359157
                                                                                                                                       |                                                                                                                                      
gaaaCAGTGCTATTTACCCGCAGAGTGAAAATAACCAGCAACCCGATGACCGCCACCGCTGCCCGCGTCAGTAAGCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGcc                                                                                                                                    <  2:807518/138‑1 (MQ=255)
  aaCAGTGCGATTTACACGCAGAGTGAAAATAATCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCa                                                                                                                                  <  1:526443/140‑1 (MQ=255)
    cAGTGCGATTTACACGCAGAGTGAAAATAATCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACt                                                                                                                                >  1:807518/1‑140 (MQ=255)
    cAGTGCGATTTACACGCAGAGTGAAAATAATCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACt                                                                                                                                >  1:553639/1‑140 (MQ=255)
       tGCGATTTACACGCAGAGTGAAAATAATCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCa                                                                                                                             >  1:150060/1‑140 (MQ=255)
         cGATTTACACGCAGAGTGAAAATAATCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCAcc                                                                                                                           >  1:175033/1‑140 (MQ=255)
            tttACACGCAGAGTGAAAATAATCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCaaa                                                                                                                        >  1:751855/1‑140 (MQ=255)
            tttACACGAAGAGTAAAAATAACCAGCAAACCGATGACCGCCCCCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCaaa                                                                                                                        <  2:508760/140‑1 (MQ=255)
              tACACGCAGAGTGAAAATAATCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACa                                                                                                                      >  2:62122/1‑140 (MQ=255)
                      gagTGAAAATAATCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTa                                                                                                                >  2:103298/1‑138 (MQ=255)
                      gagTGAAAATAATCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTa                                                                                                                <  1:103298/138‑1 (MQ=255)
                       agTGAAAATAATCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACaa                                                                                                             <  1:358741/140‑1 (MQ=255)
                             aataacCACGGACCCGATGCCCGCCCCCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCaa                                                                                                       <  2:150060/140‑1 (MQ=255)
                                 atCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTcc                                                                                                   >  1:334713/1‑140 (MQ=255)
                                 atCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTcc                                                                                                   >  2:503155/1‑140 (MQ=255)
                                 atCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTcc                                                                                                   >  1:418317/1‑140 (MQ=255)
                                     gCGACCCGATGACCGCCCCCGCTGCCAGCGTCAGTACTCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGca                                                                                               <  2:553639/140‑1 (MQ=255)
                                       gacccGATGACCGCCCCCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGcagc                                                                                             <  1:597878/137‑1 (MQ=255)
                                            gATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAgcg                                                                                        <  2:593635/140‑1 (MQ=255)
                                            gATGACCACCACCACTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAgcg                                                                                        <  1:494941/140‑1 (MQ=255)
                                             aTGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAgcgc                                                                                       >  1:756803/1‑140 (MQ=255)
                                                  cGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTAc                                                                                  >  2:52111/1‑140 (MQ=255)
                                                    ccACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTatcg                                                                                <  2:418317/140‑4 (MQ=255)
                                                    ccACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCg                                                                                <  2:756803/140‑1 (MQ=255)
                                                        cGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGa                                                                              >  2:703327/1‑138 (MQ=255)
                                                        cGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGa                                                                              <  1:703327/138‑1 (MQ=255)
                                                            gCCAGCTTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGaa                                                                             >  2:780180/1‑135 (MQ=255)
                                                            gCCAGCTTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGaa                                                                             <  1:780180/135‑1 (MQ=255)
                                                            gCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAg                                                                        <  1:503155/140‑1 (MQ=255)
                                                            gCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAg                                                                        <  1:2559/140‑1 (MQ=255)
                                                             ccAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGt                                                                       <  1:62122/140‑1 (MQ=255)
                                                                 cGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCg                                                                                <  2:553552/127‑1 (MQ=255)
                                                                 cGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCg                                                                                >  1:553552/1‑127 (MQ=255)
                                                                       tAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCa                                                             <  1:790150/140‑1 (MQ=255)
                                                                       tAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCa                                                             >  2:572464/1‑140 (MQ=255)
                                                                       tAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCa                                                             <  2:334713/140‑1 (MQ=255)
                                                                       aaaccccGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTTTGTCGCCa                                                             <  2:260717/136‑1 (MQ=255)
                                                                       aaaTCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCa                                                             <  2:204746/139‑1 (MQ=255)
                                                                            ccgccgCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTc                                                        >  1:96203/1‑140 (MQ=255)
                                                                              gccgcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTc                                                        >  2:385920/1‑138 (MQ=255)
                                                                              gccgcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTc                                                        <  1:385920/138‑1 (MQ=255)
                                                                               ccgcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATc                                                           >  1:719399/1‑134 (MQ=255)
                                                                               ccgcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATc                                                           <  2:719399/134‑1 (MQ=255)
                                                                               ccgcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAg                                                     >  1:748845/1‑140 (MQ=255)
                                                                               ccgcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAg                                                     >  2:166088/1‑140 (MQ=255)
                                                                                 gcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGaa                                                   <  2:453967/140‑1 (MQ=255)
                                                                                  cATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCGTCGTCCAGAac                                                  <  2:228587/140‑1 (MQ=255)
                                                                                                    tttCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATa                                >  1:668692/1‑140 (MQ=255)
                                                                                                      tCTGCTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATaaa                              >  1:635/1‑140 (MQ=255)
                                                                                                          cTTTCACGCGCAGGATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCg                          >  1:723173/1‑140 (MQ=255)
                                                                                                                      ggATCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAaca              <  1:572464/140‑1 (MQ=255)
                                                                                                                        aTCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAacaca            <  2:5676/140‑1 (MQ=255)
                                                                                                                        aTCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAacaca            <  2:723173/140‑1 (MQ=255)
                                                                                                                        aTCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAacaca            <  1:231578/140‑1 (MQ=255)
                                                                                                                        aTCGGGGCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAacaca            <  1:52111/140‑1 (MQ=255)
                                                                                                                           ggggCAATAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAg         >  1:305725/1‑140 (MQ=255)
                                                                                                                             ggCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgcg       >  2:782909/1‑140 (MQ=255)
                                                                                                                                  tAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACg  <  1:166088/140‑1 (MQ=255)
                                                                                                                                  tAAACACGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTACAACACACAGCGCTACg  >  2:608843/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                      
GCAACAGTGCGATTTACACGCAGAGTGAAAATAATCAGCGAACCGATGACCGCCACCGCTGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACG  >  NC_000913/2358888‑2359157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A