Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A30 F30 I1 R1 25 26.9 915936 99.4% 910440 139.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,492,306 G→T L74M (CTG→ATG)  idnT ← L‑idonate/5‑ketogluconate/gluconate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,492,3060GT100.0% 101.5 / NA 30L74M (CTG→ATG) idnTL‑idonate/5‑ketogluconate/gluconate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (10/20);  total (10/20)

CAAAACCCACTTCAAAAAACATGGCGAGGCCCACAACCAGACCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACG  >  NC_000913/4492171‑4492442
                                                                                                                                       |                                                                                                                                        
cAAAACCCACTTCAAAAAACATGGCGAGGCCCACAACCAGACCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTg                                                                                                                                      <  1:184829/140‑1 (MQ=255)
               aaaaCATGGCGAGGCCCTCAACCAGACCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCg                                                                                                                       <  2:12929/140‑1 (MQ=255)
                      ggCGAGGCCCACAACCAGACCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATAGCCCCGAAACCGa                                                                                                                <  2:111339/140‑1 (MQ=255)
                                 caACCAGACCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGcc                                                                                                     <  2:245206/140‑1 (MQ=255)
                                       gACCGGTGATCACTAGCGCCCATTGCGCGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCccg                                                                                               >  2:277653/1‑140 (MQ=255)
                                       gACCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCccg                                                                                               >  2:260929/1‑140 (MQ=255)
                                        aCCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCccgc                                                                                              >  1:189094/1‑140 (MQ=255)
                                        aCCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCccgc                                                                                              >  2:234738/1‑140 (MQ=255)
                                        aCCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCccgc                                                                                              >  1:40620/1‑140 (MQ=255)
                                                         cccATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATa                                                                             >  2:62735/1‑140 (MQ=255)
                                                         cccATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATa                                                                             <  2:226715/140‑1 (MQ=255)
                                                         cccATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATa                                                                             >  2:268795/1‑140 (MQ=255)
                                                           cATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATAcc                                                                           <  2:140904/140‑1 (MQ=255)
                                                           cATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATAcc                                                                           <  1:303158/140‑1 (MQ=255)
                                                           cATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATAcc                                                                           <  2:255016/140‑1 (MQ=255)
                                                           cATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATAcc                                                                           <  1:112375/140‑1 (MQ=255)
                                                           cATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCAGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATAcc                                                                           <  2:412484/140‑1 (MQ=255)
                                                           cATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTCCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATAcc                                                                           <  1:378910/140‑1 (MQ=255)
                                                            aTTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATa                                                                             <  2:191036/137‑1 (MQ=255)
                                                            aTTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATa                                                                             >  1:191036/1‑137 (MQ=255)
                                                             ttGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCAt                                                                         <  1:62735/140‑1 (MQ=255)
                                                             ttGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCAt                                                                         <  2:338994/140‑1 (MQ=255)
                                                              tGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCAtt                                                                        <  1:71923/140‑1 (MQ=255)
                                                                     gTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGtata                                                                 <  2:85138/140‑1 (MQ=255)
                                                                     gTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGtata                                                                 <  1:185042/140‑1 (MQ=255)
                                                                              cAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAgg                                                        <  2:166165/140‑1 (MQ=255)
                                                                                                tAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGcatccatc                                      >  1:5705/1‑140 (MQ=255)
                                                                                                               gtgCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCcg                       >  2:158075/1‑140 (MQ=255)
                                                                                                                        ccGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCa              <  2:330798/140‑1 (MQ=255)
                                                                                                                                    tCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACg  <  2:5705/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
CAAAACCCACTTCAAAAAACATGGCGAGGCCCACAACCAGACCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACG  >  NC_000913/4492171‑4492442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A