Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F30 I1 R1	23	42.7	1455564	99.2%	1443919	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,235,936	G→T	S224S (TCG→TCT)	preA →	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,235,936	0	G	T	100.0%	154.6 / NA	44	S224S (TCG→TCT)	preA	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (25/19); total (25/19)

GTGATATGTGCGAAGTGGCGCTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTGGGAGGATGCGGCTG  >  NC_000913/2235803‑2236072

gTGATATGTGCGAAGTGGCGCTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTAtt                                                                                                                                    >  1:596557/1‑140 (MQ=255)

   atatGTGCGAAGTGGCGCTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTcc                                                                                                                                 >  2:88399/1‑140 (MQ=255)

      tgtgCGAAGTGGCGCTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGa                                                                                                                              >  2:69841/1‑140 (MQ=255)

      tgtgCGAAGTGGCGCTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGa                                                                                                                              <  1:98488/140‑1 (MQ=255)

        tgCGAAGTGGCGCTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGata                                                                                                                            >  2:652937/1‑140 (MQ=255)

         gCGAAGTGGCGCTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGatat                                                                                                                           <  1:88399/140‑1 (MQ=255)

          cGAAGTGGCGCTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATAtt                                                                                                                          >  1:14888/1‑140 (MQ=255)

                  cgcTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTaaa                                                                                                                  >  1:682161/1‑140 (MQ=255)

                  cgcTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTaaa                                                                                                                  >  2:607325/1‑140 (MQ=255)

                      ggcggcGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTaa                                                                                                                   <  2:604061/135‑1 (MQ=255)

                      ggcggcGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTaa                                                                                                                   >  1:604061/1‑135 (MQ=255)

                          gcgAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTaaa                                                                                                          <  1:69841/140‑1 (MQ=255)

                               gcgcGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGa                                                                                                     <  2:121610/140‑1 (MQ=255)

                                cgcgGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGAt                                                                                                    <  2:596557/140‑1 (MQ=255)

                                  cggcggcgCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCg                                                                                                  >  2:272152/1‑140 (MQ=255)

                                    gcggcgCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGcc                                                                                                <  1:373460/140‑1 (MQ=255)

                                    gcggcgCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGcc                                                                                                <  1:272152/140‑1 (MQ=255)

                                                              aaCACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCAc                                                                      <  1:595675/140‑1 (MQ=255)

                                                                    gTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATcc                                                                >  2:679261/1‑140 (MQ=255)

                                                                    gTTAAATCCATCACCAATACCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATcc                                                                >  1:149485/1‑140 (MQ=255)

                                                                     ttAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCAc                                                                      <  1:75388/133‑1 (MQ=255)

                                                                     ttAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCAc                                                                      <  1:649756/133‑1 (MQ=255)

                                                                     ttAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCAc                                                                      >  2:75388/1‑133 (MQ=255)

                                                                     ttAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCAc                                                                      >  2:649756/1‑133 (MQ=255)

                                                                     ttAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCa                                                               >  2:208089/1‑140 (MQ=255)

                                                                               cACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGcgcg                                                     <  1:607325/140‑1 (MQ=255)

                                                                               cACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGcgcg                                                     >  2:599960/1‑140 (MQ=255)

                                                                                          aTCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCaa                                            <  2:255422/138‑1 (MQ=255)

                                                                                          aTCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCaa                                            >  1:255422/1‑138 (MQ=255)

                                                                                          aTCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATc                                          >  2:114410/1‑140 (MQ=255)

                                                                                            cTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAg                                        >  2:657001/1‑140 (MQ=255)

                                                                                               aaTCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCgg                                     >  1:452744/1‑140 (MQ=255)

                                                                                                 tCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTa                                   >  1:373870/1‑140 (MQ=255)

                                                                                                            gTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCAtt                        <  1:599960/140‑1 (MQ=255)

                                                                                                               tGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATcggcg                             >  2:9226/1‑132 (MQ=255)

                                                                                                               tGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATcggcg                             <  1:9226/132‑1 (MQ=255)

                                                                                                               tGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGaa                     >  1:368136/1‑140 (MQ=255)

                                                                                                                    aTCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTg                >  1:648410/1‑140 (MQ=255)

                                                                                                                     tCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTgg               <  1:665683/140‑1 (MQ=255)

                                                                                                                      cGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTggg              >  2:307146/1‑140 (MQ=255)

                                                                                                                         tAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTGggagg           <  2:14888/140‑1 (MQ=255)

                                                                                                                               aaaaTCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTGGGAGGATGCgg     <  1:657001/140‑1 (MQ=255)

                                                                                                                                 aaTCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTGGGAGGATGCGGCt   >  1:48340/1‑140 (MQ=255)

                                                                                                                                  aTCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTGGGAGGATGCGGCTg  <  2:149485/140‑1 (MQ=255)

GTGATATGTGCGAAGTGGCGCTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTGGGAGGATGCGGCTG  >  NC_000913/2235803‑2236072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A