Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A25 F30 I1 R1	18	41.0	1418494	98.5%	1397216	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,255,245	G→T	intergenic (‑59/‑110)	rihB ← / → yeiL	pyrimidine‑specific ribonucleoside hydrolase RihB/putative DNA‑binding transcriptional regulator YeiL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,255,245	0	G	T	100.0%	162.6 / NA	47	intergenic (‑59/‑110)	rihB/yeiL	pyrimidine‑specific ribonucleoside hydrolase RihB/putative DNA‑binding transcriptional regulator YeiL
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (17/30); total (17/30)

GATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATTGATGAGTGAATCCGCGTTT  >  NC_000913/2255108‑2255372

gATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCtgg                                                                                                                               >  2:95948/1‑140 (MQ=255)

     ttCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCgat                                                                                                                          >  2:476530/1‑140 (MQ=255)

           gccATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGa                                                                                                                    <  2:191026/140‑1 (MQ=255)

           gccATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGa                                                                                                                    <  1:698567/140‑1 (MQ=255)

           gccATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGa                                                                                                                    <  1:643337/140‑1 (MQ=255)

                catTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCtt                                                                                                               <  1:643154/140‑1 (MQ=255)

                     tagcaatagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTaacaa                                                                                                          <  2:36842/140‑1 (MQ=255)

                     tagcaatagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTaacaa                                                                                                          <  1:673324/140‑1 (MQ=255)

                          atagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGcg                                                                                                     >  2:98462/1‑140 (MQ=255)

                           tagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGcgt                                                                                                    >  2:431045/1‑140 (MQ=255)

                           tagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGcgt                                                                                                    >  2:596315/1‑140 (MQ=255)

                                  atcatGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATg                                                                                             >  1:542802/1‑140 (MQ=255)

                                   tcatGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGcgtacg                                                                                                 <  1:451743/135‑1 (MQ=255)

                                   tcatGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGcgtacg                                                                                                 >  2:451743/1‑135 (MQ=255)

                                     atGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTa                                                                                          <  1:476530/140‑1 (MQ=255)

                                             gATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATgag                                                                                  >  1:38654/1‑140 (MQ=255)

                                             gATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATgag                                                                                  <  2:480992/140‑1 (MQ=255)

                                             gATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATgag                                                                                  <  2:542802/140‑1 (MQ=255)

                                                  caATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAAtc                                                                             >  1:536969/1‑140 (MQ=255)

                                                        aGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAg                                                                       >  1:376461/1‑140 (MQ=255)

                                                          aataatTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCg                                                                     >  1:318159/1‑140 (MQ=255)

                                                          aataatTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCg                                                                     >  2:217900/1‑140 (MQ=255)

                                                               tttttCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATaa                                                                >  1:29872/1‑140 (MQ=255)

                                                                             aTTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTgaaagaa                                                  <  2:470803/140‑1 (MQ=255)

                                                                             aTTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTgaaagaa                                                  <  1:27696/140‑1 (MQ=255)

                                                                              ttGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTgaaagaaa                                                 >  2:661645/1‑140 (MQ=255)

                                                                                gTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGCTAAACATCGTGAAAGAAATc                                               <  2:376461/140‑1 (MQ=255)

                                                                                 tttATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATcc                                              >  1:329141/1‑140 (MQ=255)

                                                                                  ttATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCca                                             <  1:660887/140‑1 (MQ=255)

                                                                                  ttATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCAACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCca                                             <  1:596315/140‑1 (MQ=255)

                                                                                        ccTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACaataa                                       <  1:95948/140‑1 (MQ=255)

                                                                                        ccTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACaataa                                       <  2:329141/140‑1 (MQ=255)

                                                                                        ccTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACaataa                                       <  1:187439/140‑1 (MQ=255)

                                                                                        ccTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACaataa                                       <  1:431131/140‑1 (MQ=255)

                                                                                           ctGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACaata                                        >  1:435705/1‑136 (MQ=255)

                                                                                           ctGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACaata                                        <  2:435705/136‑1 (MQ=255)

                                                                                                tCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTa                               <  2:135953/140‑1 (MQ=255)

                                                                                                tCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTa                               >  2:233255/1‑140 (MQ=255)

                                                                                                   aGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAagc                            <  1:661645/140‑1 (MQ=255)

                                                                                                   aGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAagc                            <  1:233255/140‑1 (MQ=255)

                                                                                                           tATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATtg                    <  2:38654/140‑1 (MQ=255)

                                                                                                           tATTTTTTCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATtg                    <  2:29872/140‑1 (MQ=255)

                                                                                                                  tCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATtg                    >  2:415437/1‑133 (MQ=255)

                                                                                                                  tCTACAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATtg                    <  1:415437/133‑1 (MQ=255)

                                                                                                                     aCAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATTGATGAGTGAAt          <  2:318159/140‑1 (MQ=255)

                                                                                                                      cAGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATTGATGAGTGAATc         <  2:536969/140‑1 (MQ=255)

                                                                                                                       aGCAAAGAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATTGATGAGTGAATcc        <  2:250797/140‑1 (MQ=255)

                                                                                                                             gAAAATTCGACCTGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATTGATGAGTGAATCCGCGttt  <  1:431045/140‑1 (MQ=255)

GATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATAATGATCTTAAGCAGCAATTGATGAGTGAATCCGCGTTT  >  NC_000913/2255108‑2255372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A