Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F30 I1 R1	23	42.7	1455564	99.2%	1443919	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,037,861	G→T	A82A (GCC→GCA)	recJ ←	ssDNA‑specific exonuclease RecJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,037,861	0	G	T	100.0%	114.1 / NA	34	A82A (GCC→GCA)	recJ	ssDNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (16/18); total (16/18)

TGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCAC  >  NC_000913/3037736‑3037997

tGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCAcc                                                                                                                            <  2:361911/140‑1 (MQ=255)

tGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCAcc                                                                                                                            <  1:667810/140‑1 (MQ=255)

 gATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCg                                                                                                                           >  2:356877/1‑140 (MQ=255)

      aCCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGa                                                                                                                          >  2:723151/1‑136 (MQ=255)

      aCCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGa                                                                                                                          <  1:723151/136‑1 (MQ=255)

        cACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACaa                                                                                                                    <  2:59580/140‑1 (MQ=255)

             ccGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCCTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATc                                                                                                               >  2:590209/1‑140 (MQ=255)

                     aaGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTcc                                                                                                       >  1:284357/1‑140 (MQ=255)

                     aaGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTcc                                                                                                       >  1:388905/1‑140 (MQ=255)

                           tAACCGTCTTCGAAACGGCTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTcgcg                                                                                                 >  1:644046/1‑140 (MQ=255)

                            aaCCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGa                                                                                                >  2:369187/1‑140 (MQ=255)

                                gTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAg                                                                                            <  1:369187/140‑1 (MQ=255)

                                 tCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCt                                                                                                      <  2:693143/129‑1 (MQ=255)

                                 tCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCt                                                                                                      >  1:693143/1‑129 (MQ=255)

                                  cTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCg                                                                                          <  2:388905/140‑1 (MQ=255)

                                  cTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCg                                                                                          <  1:716527/140‑1 (MQ=255)

                                  cTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCg                                                                                          >  1:382660/1‑140 (MQ=255)

                                  cTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCg                                                                                          >  2:86129/1‑140 (MQ=255)

                                   ttCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGt                                                                                         <  2:58320/140‑1 (MQ=255)

                                    tCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGtt                                                                                        <  2:541659/140‑1 (MQ=255)

                                       aaaCGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTa                                                                                     >  2:488295/1‑140 (MQ=255)

                                         aCGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTaaa                                                                                   <  1:488295/140‑1 (MQ=255)

                                               tGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCg                                                                                          <  1:9099/127‑1 (MQ=255)

                                               tGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCg                                                                                          >  2:9099/1‑127 (MQ=255)

                                                                tatTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGAcg                                                            >  1:245172/1‑140 (MQ=255)

                                                                     ctgcAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCt                                                       >  1:470237/1‑140 (MQ=255)

                                                                          aCCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGtt                                                  <  1:644304/140‑1 (MQ=255)

                                                                          aCCAAGCGAGCGCAACGCCCGCATGCTTAGAGTCGTAATGGAAGTGGCTGATAGGTCTATATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGCTGTAAAGGATCTCAACGGCCTGTTCGACGCCGCTCAGtt                                                  <  2:644046/140‑1 (MQ=255)

                                                                                 gagcgCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCa                                           <  1:75283/140‑1 (MQ=255)

                                                                                          gCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCAt                                  <  2:23229/140‑1 (MQ=255)

                                                                                                      agagCCGTGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACt                      <  1:356877/140‑1 (MQ=255)

                                                                                                          ccGTGCTGGTGCCGCCGAGTGCGTCGAAATCACCGACCACAATACTCCGCAGTCCGTCGCGAAAAGCGTTGTAAAGCAGCTCAACGGCCTTTTCGACGCCGCCCAGCTGCTGCCAGGGCAGCATACCTTTAACACTGCGt                  >  2:73372/1‑140 (MQ=255)

                                                                                                             tGCTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTcc               >  1:109198/1‑140 (MQ=255)

                                                                                                               cTGGTCGCGCCGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAg             <  2:109198/140‑1 (MQ=255)

                                                                                                                         cGTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCa   >  2:311051/1‑140 (MQ=255)

                                                                                                                          gTCTGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCAc  <  1:96548/140‑1 (MQ=255)

TGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCGACCACAATAATCCGCGTTCCTTCGCGAAAAGCGTTGTAAAGGATCTCAACGGCCTTTTCGACGCCGCTCAGTTGCTGCCAGGGCAGCATACCTTTAACACTGCGTTCCAGTTCTTGCGCAC  >  NC_000913/3037736‑3037997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A