Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A36 F30 I1 R1	19	98.9	3364616	99.3%	3341063	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,777,746	G→T	R98S (AGG→AGT)	ypjF →	CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,777,746	0	G	T	99.1%	381.2 / ‑4.1	109	R98S (AGG→AGT)	ypjF	CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (66/42); minor base A (0/1); total (66/43)
Fisher's exact test for biased strand distribution p-value = 3.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

Only 100 of 109 total aligned reads displayed.

ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGACATT  >  NC_000913/2777613‑2777882

aTGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGctct                                                                                                                                    >  2:1680118/1‑140 (MQ=255)

 tGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTa                                                                                                                                   >  2:71348/1‑140 (MQ=255)

 tGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTa                                                                                                                                   <  1:267584/140‑1 (MQ=255)

  gCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTAc                                                                                                                                  >  1:1513556/1‑140 (MQ=255)

    tGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCg                                                                                                                                >  1:1087775/1‑140 (MQ=255)

    tGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCg                                                                                                                                >  1:1396169/1‑140 (MQ=255)

    tGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCg                                                                                                                                >  1:528835/1‑140 (MQ=255)

      gTATCACTCTGGCCGATGCAGTCAATTTTCTCGTGGAAAAGTACGAACTGGTACGCAGCGATCACAGAGGATTTTCCTCGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGc                                                                                                                              <  2:1300497/140‑1 (MQ=255)

       tATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCt                                                                                                                             <  1:900795/140‑1 (MQ=255)

        aTCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCtt                                                                                                                            >  1:602163/1‑140 (MQ=255)

           aCTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCt                                                                                                                         <  2:1087775/140‑1 (MQ=255)

           aCTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCt                                                                                                                         >  2:890007/1‑140 (MQ=255)

           aCTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCt                                                                                                                         >  2:526767/1‑140 (MQ=255)

           aCTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCt                                                                                                                         >  2:495385/1‑140 (MQ=255)

           aCTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCt                                                                                                                         <  1:988221/140‑1 (MQ=255)

              ctGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTaaa                                                                                                                      >  1:751823/1‑140 (MQ=255)

               tGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTaaaa                                                                                                                     >  1:221606/1‑140 (MQ=255)

                ggCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTaaaaa                                                                                                                    <  2:1308000/140‑1 (MQ=255)

                ggCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTaaaaa                                                                                                                    >  1:1300497/1‑140 (MQ=255)

                 gCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAAc                                                                                                                   >  2:1571518/1‑140 (MQ=255)

                 gCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAAc                                                                                                                   <  2:420857/140‑1 (MQ=255)

                 gCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAAc                                                                                                                   >  2:416683/1‑140 (MQ=255)

                      tGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAAtg                                                                                                              >  2:180118/1‑140 (MQ=255)

                      tGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAAtg                                                                                                              <  1:1680118/140‑1 (MQ=255)

                           tCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGaaa                                                                                                         >  1:620233/1‑140 (MQ=255)

                           tCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGaaa                                                                                                         >  1:1474067/1‑140 (MQ=255)

                               ttttCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATaaa                                                                                                     <  2:401340/140‑1 (MQ=255)

                               ttttCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATaaa                                                                                                     <  2:1511369/140‑1 (MQ=255)

                               ttttCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATaaa                                                                                                     <  1:526767/140‑1 (MQ=255)

                               ttttCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATaaa                                                                                                     <  2:685898/140‑1 (MQ=255)

                               ttttCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATaaa                                                                                                     <  2:620233/140‑1 (MQ=255)

                               ttttCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGACGCTCTACCGGCTTGCTAAAAACTAATGTGAAATaaa                                                                                                     <  2:410932/140‑1 (MQ=255)

                                    tggtggAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAAt                                                                                                               <  1:1514468/125‑1 (MQ=255)

                                    tggtggAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAAt                                                                                                               >  2:1514468/1‑125 (MQ=255)

                                    tggtggAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCtt                                                                                                <  2:602163/140‑1 (MQ=255)

                                    tggtggAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCtt                                                                                                <  1:248400/140‑1 (MQ=255)

                                           aaaGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACa                                                                                         >  1:866826/1‑140 (MQ=255)

                                           aaaGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACa                                                                                         <  1:973353/140‑1 (MQ=255)

                                              gTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACagag                                                                                      <  1:180118/140‑1 (MQ=255)

                                              gTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACagag                                                                                      <  2:1474067/140‑1 (MQ=255)

                                              gTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACagag                                                                                      <  1:495385/140‑1 (MQ=255)

                                              gTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACagag                                                                                      <  1:751534/140‑1 (MQ=255)

                                                aCGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCa                                                                                    <  2:602688/140‑1 (MQ=255)

                                                 cGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAg                                                                                   >  2:540717/1‑140 (MQ=255)

                                                         tACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAgg                                                                           >  2:253926/1‑140 (MQ=255)

                                                         tACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAgg                                                                           >  1:1039847/1‑140 (MQ=255)

                                                         tACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAgg                                                                           >  2:718505/1‑140 (MQ=255)

                                                         tACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAgg                                                                           >  1:528893/1‑140 (MQ=255)

                                                             tATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAg                                                                       >  1:1595294/1‑140 (MQ=255)

                                                                  aTCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGa                                                                          <  2:501957/132‑1 (MQ=255)

                                                                  aTCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGa                                                                          >  1:501957/1‑132 (MQ=255)

                                                                  aTCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCaaaa                                                                  >  1:150187/1‑140 (MQ=255)

                                                                  aTCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCaaaa                                                                  >  2:67439/1‑140 (MQ=255)

                                                                  aTCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCaaaa                                                                  >  1:1610385/1‑140 (MQ=255)

                                                                  aTCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCaaaa                                                                  >  2:1670683/1‑140 (MQ=255)

                                                                   tCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAAt                                                                 >  2:1245289/1‑140 (MQ=255)

                                                                       agagGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTa                                                             <  1:1669524/140‑1 (MQ=255)

                                                                             ttttCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCa                                                       <  2:1039847/140‑1 (MQ=255)

                                                                             ttttCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCa                                                       >  2:838385/1‑140 (MQ=255)

                                                                             ttttCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCa                                                       >  1:223892/1‑140 (MQ=255)

                                                                             ttttCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCa                                                       >  1:466264/1‑140 (MQ=255)

                                                                             ttttCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCa                                                       >  2:1163761/1‑140 (MQ=255)

                                                                               ttCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATa                                                     <  1:113680/140‑1 (MQ=255)

                                                                               ttCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATa                                                     <  2:751823/140‑1 (MQ=255)

                                                                                   tGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACg                                                 >  1:1188153/1‑140 (MQ=255)

                                                                                          aacaGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGAc                                          <  1:1571518/140‑1 (MQ=255)

                                                                                            caGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTc                                        <  1:540717/140‑1 (MQ=255)

                                                                                             aGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTcc                                       <  1:1163761/140‑1 (MQ=255)

                                                                                                ctcCATATATTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGc                                    <  2:1595294/140‑1 (MQ=255)

                                                                                                    atatatTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTa                                <  2:1610385/140‑1 (MQ=255)

                                                                                                    atatatTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTa                                >  2:1646369/1‑140 (MQ=255)

                                                                                                    atatatTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTa                                <  2:866826/140‑1 (MQ=255)

                                                                                                    atatatTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTa                                >  1:1528441/1‑140 (MQ=255)

                                                                                                    atatatTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACCCCCGCt                                   >  1:1380036/1‑137 (MQ=255)

                                                                                                      atatTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAAc                              <  2:223892/140‑1 (MQ=255)

                                                                                                      atatTTCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAAc                              <  2:466264/140‑1 (MQ=255)

                                                                                                         tttCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAAcctc                           >  1:429010/1‑140 (MQ=255)

                                                                                                           tCCGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAAcctccc                         >  2:695523/1‑140 (MQ=255)

                                                                                                             cGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAAcctcccct                       >  2:145950/1‑140 (MQ=255)

                                                                                                             cGTAGTAGATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAAcctccccc                       >  2:849724/1‑139 (MQ=255)

                                                                                                               tagtagATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTcc                                       <  2:610428/122‑1 (MQ=255)

                                                                                                               tagtagATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTcc                                       >  1:610428/1‑122 (MQ=255)

                                                                                                               tagtagATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAAcctcccctcc                     >  1:1002262/1‑140 (MQ=255)

                                                                                                                 gtagATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCtcccctgccc                   >  1:1530708/1‑140 (MQ=255)

                                                                                                                    gATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACctcccctccc                    <  2:1431584/136‑1 (MQ=255)

                                                                                                                    gATATTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGa                >  2:524138/1‑140 (MQ=255)

                                                                                                                       atTCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGaaaa             <  1:1245289/140‑1 (MQ=255)

                                                                                                                         tCTGCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGaaaaaa           >  1:1058668/1‑140 (MQ=255)

                                                                                                                            gCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAGATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCt        >  2:1463138/1‑140 (MQ=255)

                                                                                                                            gCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCt        >  2:797087/1‑140 (MQ=255)

                                                                                                                            gCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCt        >  1:99994/1‑140 (MQ=255)

                                                                                                                            gCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCt        >  2:1064547/1‑140 (MQ=255)

                                                                                                                            gCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCt        >  1:455167/1‑140 (MQ=255)

                                                                                                                            gCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCt        >  2:357708/1‑140 (MQ=255)

                                                                                                                            gCGAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCt        >  2:234041/1‑140 (MQ=255)

                                                                                                                              gAGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGa      >  2:612329/1‑140 (MQ=255)

                                                                                                                               aGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGAc     >  2:1244897/1‑140 (MQ=255)

                                                                                                                               aGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGAc     >  2:1393320/1‑140 (MQ=255)

                                                                                                                               aGCAAGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGAc     >  1:1451652/1‑140 (MQ=255)

                                                                                                                                  aaGTCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGACAtt  >  1:1264127/1‑140 (MQ=255)

ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTTTCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAATGTGAAATAAACGCTTAAATACAGAGCAGACTGAAGGAAAGCAAAATGCTAATCTCATAGACGAAGAGACTCCCGCTGTAACCTCCCCTCCCCCGAAAAAACCTGACATT  >  NC_000913/2777613‑2777882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A